EFFORTS IN IMPROVING TABLETS’ TENSILE STRENGTH WITH A COATING SYSTEM AND BRITTLE EXCIPIENT: A REVIEW / الجهود المبذولة في تحسين قوة شد الأقراص باستخدام نظام تغليف وسواغ هش: بحث مرجعى
قطاع الدراسات الصيدلية / الصيدلانيات
تخصص البحث : الصيدلانيات
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 14
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ أحمد عينوروفيك - مؤلف رئيسي
الصيدلانيات
Tablets are the most in-demand pharmaceutical preparation by pharmaceutical companies or users because they provide the best capabilities from all oral preparations. Maintaining the grade and quality of tablets need to be of great concern. Factors affecting the physical quality of tablets must always be evaluated, including tensile strength. Tensile strength directly affects the process of distribution, storage, disintegration, and dissolution of tablets. Tensile strength problem is often found during tablet production with the wet granulation method, in which over-granulation often occurs, especially in high-shear wet granulation (HSWG). The solution for over granulating is the addition of brittle excipients to form a strong bond when combined, thus increasing tensile strength. An ideal tensile strength may be achieved because the mechanical strength of the tablet is increased proportionally to the increased area of the bond. Another solution to increase tensile strength is by using a coating system using the Multiple Unit Pellet Systems (MUPS). The MUPS coating system often experiences problems such as caking. This problem can be overcome using a coating polymer that is stable on high RH and supports a strong tablet formulation. The solution to the tensile strength problem with the addition of brittle excipients and coating will be reviewed in this article to increase tensile strength. Keywords: Tablet, wet granulation, tensile strength, brittle excipient, coating system
SODIUM ALGINATE FILM INCORPORATED WITH ZINC OXIDE NANOPARTICLES AS ANTIBACTERIAL AGENT / القدرة المضادة للجراثيم لأفلام ألجينات الصوديوم المحملة بأكسيد الزنك النانوي
قطاع الدراسات الصيدلية / الصيدلانيات
تخصص البحث : الصيدلانيات
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 10
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ وسام زم - مؤلف رئيسي
الصيدلانيات
Alginate biopolymer has been used in the design and development of several wound dressing materials such as (foams, topical formulations, nanofibers, films) due to its favorable properties, such as biocompatibility and non-toxicity. It has been particularly attractive in wound healing applications to date. Additionally, it can be loaded by antibacterial materials such as antibiotics, antibacterial plant extracts and metal nano-oxides. The aim of this study was to design sodium alginate (SA) based zinc oxide (ZnO) nanoparticles as antibacterial film for wound healing applications. The ZnO nanoparticles, with an average particle size of about 16.75 nm, were synthesized by sol-gel method and characterized by scanning electron microscope (SEM) and Fourier-transform infrared spectroscopy (FTIR). They were then incorporated as antibacterial into sodium alginate with different concentration (0, 5, 7.5, 10%). Effectiveness of antibacterial activity was carried out against Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa) and Gram-positive bacteria (Staphylococcus aureus) to characterize the sample. The prepared ZnO/SA film presents a promising potential for applications as novel wound dressings Keywords: zinc oxide nanoparticles; sodium alginate; sol-gel method; antibacterial film; wound dressing
ADVANCES AND DEVELOPMENTS IN DRUG REPURPOSING; STATE OF ART / أوجه التقدم والتطورات في مجال إعادة استخدام الأدوية
قطاع الدراسات الصيدلية / الصيدلانيات
تخصص البحث : الصيدلانيات
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 10
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ سرمد ضياء نوري - مؤلف رئيسي
الصيدلانيات
Although the rapid progress in science and technology, developing new drugs from scratch has been expensive in recent decades. As a result, drug-repurposing, which implies seeking new utilizes for presently approved drugs instead of discovering new drug compounds, has emerged as a substitution tool to speed up the drug development procedure. Nowadays, drug repurposing accounts for 30% of newly approved drugs. With the explosive growth of pharmacological compounds’ molecular, genomic, and phenotypic data, a novel area of drug repurposing called computational drug repurposing has emerged. This review provides an overview of recent progress in computational drug repurposing, including repositioning approaches, commonly used computational methods, validation techniques for repurposing studies, and the remaining encounters in computational repurposing. In this Review, we outline methods for drug repurposing (also known as drug repositioning), talk about difficulties encountered by the repurposing community, and suggest creative solutions to these issues. addressed to aid in maximising the benefits of medicinal repurposing. Keywords: Drug repurposing, nanosystem, anticancer, bacterial infection, fungal infection
EVALUATION AND PREPARATION OF ATENOLOL-LOADED FLOATING MICROCAPSULES / تحضير وتوصيف حويصلات دقيقه ممتده المفعول حامله لدواء الاتينولول
قطاع الدراسات الصيدلية / الصيدلانيات
تخصص البحث : الصيدلانيات
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 11
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ حيدر حمودي - مؤلف رئيسي
الصيدلانيات
The aim of this investigation is to prepare Atenolol (AT) floating microcapsules with sustained release profiles utilizing ethyl cellulose (EC) as a matrix former. EC is frequently used as a hydrophobic polymeric material for extended drug release applications. Emulsion-solvent evaporation technique was utilized to fabricate the floating microcapsules. The ratios of EC and AT utilized were 1:1, 1:2, 1:3, 1:4, and 1:5, with the amount of EC used being constant and the amount of AT being variable depending on the polymer ratio/AT ratio. The prepared microcapsules were assessed for entrapment efficiency (EE%), drug loading, percent yield (% yield), buoyancy percentage (%), particle size analysis, and in-vitro drug release study after 12 h. After that, the best-achieved microcapsule was characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and X-ray diffraction (XRD). The best formula (M1) demonstrated a spherical morphology. Further, M1 released about 91.23 ± 3.21% after 12 h. Moreover, the FTIR showed that their no chemical interaction between the formulation components. In addition, DSC showed the ability of microcapsules to enclose AT inside its constructs. Further, XRD studies showed that microcapsules had an amorphous form. The previous findings demonstrated the good capability of M1 as a floating dosage form for sustained and continuous drug delivery of AT. Keywords: Atenolol, Floating dosage form, microcapsules, XRD, SEM
RESVERATROL LIPID BASED NANOPARTICLES SHOW A PROMISING OUTCOME ON INDUCED NON ALCOHOLIC FATTY LIVER DISEASE / اظهار نتائج واعدة على مرض الكبد الدهني غير الكحولي باستخدام صواغات دهنية نانومترية تحتوي على ريسفيراترول
قطاع الدراسات الصيدلية / الصيدلانيات
تخصص البحث : الصيدلانيات
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 13
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ دينا عادل كامل - مؤلف رئيسي
الصيدلانيات
Prevalence of nonalcoholic fatty liver disease (NAFLD) affects more than 25% of the populations worldwide. In this study, resveratrol loaded solid lipid nanoparticles (Res-SLNs) were prepared by emulsification and were in vitro evaluated. Res-SLN 1 containing gelucire and tween 80 was selected for further in vitro and in vivo studies. In vivo studies were done via induction of NAFLD in rats with a high fat (HF) diet for 12-weeks followed by a 6-week period of treatment with Res-SLN1. Blood samples and liver sections were taken from rats for biochemical and histological evaluations. Res-SLN1 exhibited high entrapment efficiency (75±22%), small particle size (208±48 nm) and an acceptable release rate. In vivo studies demonstrated a remarkable improvement in lipid profile and liver enzyme with Res-SLN1. The histological features were significantly improved with Res-SLN1. Res-SLNs represent a promising therapeutic approach for NAFLD via prolonging Res release and ameliorating lipid profile and liver enzymes. Keywords: Nonalcoholic fatty liver disease, resveratrol, solid lipid nanoparticles
POLYMERIC NANOPARTICLES AS ANTICANCER DRUG DELIVERY SYSTEMS OF CERTAIN PHYTOCHEMICALS: A COMPREHENSIVE OVERVIEW / نانوجزئيات بوليميرية كنظم لتوصيل أدوية مضادة للسرطان من المركبات الحيوية الطبيعية (الفيتوكيماويات): نظرة شاملة
قطاع الدراسات الصيدلية / الصيدلانيات
تخصص البحث : الصيدلانيات
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 27
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ شادي محمد عبد الحليم - مؤلف رئيسي
الصيدلانيات
Cancer remains a major global health concern with high fatality rates despite the existing therapeutic approaches. Although chemotherapy, radiotherapy, and surgical resection have significantly lowered cancer mortality, the survival rate remains low as a result of their adverse effects, including hepatotoxicity, cardiac cytotoxicity, nephrotoxicity, gastrointestinal toxicity, myelosuppression, mucositis, neurotoxicity, and alopecia. Natural bioactive compounds (phytochemicals) have long been explored as potential reservoirs for new efficient anticancer components that can help reduce mortality rates. They exhibit extensive structural diversity and have shown promise in targeting cancer pathways, inducing cell cycle arrest, and promoting apoptosis in preclinical studies. However, their clinical application is hindered by significant limitations, including poor aqueous solubility, low bioavailability, low gastrointestinal tract stability, and rapid clearance from the bloodstream. Polymeric nanoparticles (PNPs) have emerged as a promising solution to overcome these limitations and offering efficient delivery of phytochemicals, with substantial entrapment capacity and stability, efficient controlled release, boosted bioavailability, and remarkable therapeutic efficiency. This review article provides an overview of commonly used biodegradable polymers and their classes for preparing PNPs loaded with natural phytochemicals together with their recent anticancer research findings. Moreover, this article highlights the importance of PNPs in facilitating the effective delivery of anticancer bioactive compounds, thereby enhancing their therapeutic response while reducing side effects. Keywords: Phytochemicals; Anticancer; Polymeric nanoparticles; Biodegradable polymers.
CELECOXIB NIOSOMAL IN-SITU GEL AS A VALUABLE DRUG DELIVERY SYSTEM FOR OCULAR INFLAMMATION / الهلام المحمل بالنيوزومات المحتوية على عقار السيليكوكسيب كنظام توصيل دوائى قيم لالتهاب العين
قطاع الدراسات الصيدلية / الصيدلانيات
تخصص البحث : الصيدلانيات
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 27
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ إكرامى عبد الرحيم خليل - مؤلف رئيسي
الصيدلانيات
Niosomes are promising nanocarriers for ocular drug administration since they have the potential to enhance the bioavailability and efficacy of different drugs. Meanwhile, topical gels are beneficial for the treatment of ocular inflammation as they improve corneal permeability and increase the contact time with the eye surface. The main purpose of this study was to prepare and evaluate novel niosomal gels for intraocular delivery of celecoxib. Different niosomes were prepared using different surfactants (span 60 and span 40) and cholesterol (30-50 mol %). The optimized formulation made with span 40 and cholesterol (7:3 molar ratio) has a relatively high encapsulation efficiency (" ~ "57%) with reasonable particle size for ocular delivery (" ~ "348 nm) and showed the highest release of " ~ "65% after 24 hrs compared to other formulations. The optimized niosomal formulation was then used to prepare various formulations of celecoxib in-situ gel and topical hydrogels. The niosomal gels were well tolerated by the eye and showed similar celecoxib corneal permeation. However, the in-situ gel showed a higher anti-inflammatory effect compared to the topical hydrogel and a commercially available diclofenac eye drop. The results shown in this study indicate that celecoxib niosomal in-situ gel is a valuable drug delivery system for ocular inflammation. Keywords: Celecoxib; niosomes; niosomal in-situ gel; niosomal hydrogel; in-vivo anti-inflammatory effect
ADVANCEMENTS IN NANOPARTICLE-BASED RNA THERAPEUTICS AND RNA DELIVERY SYSTEMS / التطورات في علاجات الحمض النووي الريبي (RNA) القائمة على الجسيمات النانوية وأنظمة توصيل الحمض النووي الريبي (RNA)
قطاع الدراسات الصيدلية / الصيدلانيات
تخصص البحث : الصيدلانيات
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 17
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ ميس مازن الحمداني - مؤلف رئيسي
الصيدلانيات
Indeed, because of their potential to overcome some of the limitations of synthetic polymer-based nanoparticles, the usage of RNA-based nanoparticles has attracted substantial interest in recent years. Because RNA is a naturally occurring molecule that can be easily synthesized and manipulated to attain certain features, it is an appealing platform for the generation of nanoparticles. Drug delivery, gene therapy, and imaging have all been investigated using RNA-based nanoparticles. One of its advantages is RNA-based nanoparticles’ biocompatibility, making them less likely to produce toxicity or unfavorable effects in biological systems. Furthermore, RNA-based nanoparticles can be made to be very selective, allowing therapeutic medicines to be delivered to specific cells or tissues. This selectivity is achieved by creating RNA molecules that recognize and bind to specific targets such as cancer cells or virus particles. Because RNA-based nanoparticles are biodegradable and do not persist in the environment, they have the potential to be more environmentally friendly than synthetic polymer-based nanoparticles. As a result, the usage of RNA-based nanoparticles may lessen the potential for negative effects on aquatic and other habitats. Overall, the development of RNA-based nanoparticles has opened up new possibilities for medicinal delivery and other applications. While there are still obstacles to overcome, such as optimizing their stability and efficacy, the potential benefits of these nanoparticles make them an intriguing area of research in nanotechnology. Keywords: RNA-based nanoparticles, drug delivery, gene therapy, drug development
IN-SILICO STUDY OF CASSIA TORA LINN IN THE TREATMENT OF GAUCHER DISEASE: A SCIENTIFIC ETHNOMEDICAL STUDY / دراسة في السيليكو لكاسيا تورا لين في علاج مرض غوشيه: دراسة علمية إثنوطبية
قطاع الدراسات الصيدلية / العقاقير
تخصص البحث : العقاقير
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 13
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ د س ن ب ك براسانث - مؤلف رئيسي
العقاقير
The field of pharmacognosy and herbal medicine has been growing steadily over the years due to the increasing number of studies on the side effects of modern medicines and the development of new drug lines. Plants possess therapeutic properties. Gaucher disease is a rare disorder caused by a defective GBA1 gene, which encodes the acid-?-glucosidase enzyme. The molecular docking technique revealed good binding efficiency of the selected bioactive compounds from Cassia tora (C. tora) against the acid-?-glucosidase enzyme that causes GD. A computational approach was used to analyze the ADMET profiles of various phytocompounds using admetsar, protox-ii, and swissadme software. ADMET analysis revealed that Obtusifolin-2-glucoside and Cassiaside had good Health Impact Assessment (HIA) and showed no toxic effects. Preventive measures for GD result in side effects that are inaccessible and result in the emergence of phytocompounds with fewer toxic effects. The obtusifolin-2-glucoside and Cassiaside of C. tora exhibited good docking scores of -7.2 and -7 kcal/mol, respectively, and could be further analyzed using molecular dynamics and in vitro studies. Keywords: Obtusifolin-2-glucoside, Cassia tora Linn, Gaucher Disease, Acid-?-glucosidases, In-silico, ADMET
PHARMACOGNOSTIC, PHYTOCHEMICAL EVALUATION, AND ANTIOXIDANT ACTIVITY OF THE LEAVES OF GREWIA BILAMELLATA GAGNEP. (TILIACEAE) / تقييم عقاقيرى و كيميائي، والنشاط المضاد للأكسدة لأوراق نبات جرويا بيلاميلاتا جاجنب (زيزفونية)
قطاع الدراسات الصيدلية / العقاقير
تخصص البحث : العقاقير
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 17
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ سونيثا كى - مؤلف رئيسي
العقاقير
This research seeks to analyze the Pharmacognostic, Phytochemical evaluation, Total Phenolic, Flavonoid Content, and Antioxidant activity of the leaves of Grewia bilamellata Gagnep. (G. bilamellata). This Pharmacognostic study comprises microscopic observation, leaf characteristics, phytochemical and physicochemical analyses, and total phenolic and flavonoid content. Antioxidant activity was examined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and H2O2 assays. The leaf shows anomocytic stomata, covering, glandular and stellate type of trichomes, Total phenolic and flavonoid content turned greatest in ethanolic extract (EEGB) (90.69±2.86 mg GAE/g), and (26.21 ± 1.51 mg RE/g), respectively. EEGB showed the most intense 1,1-diphenyl-2-picrylhydrazyl (DPPH) and H2O2 scavenging activity, with IC50 59.96 and 59.24 µg/ml. Total phenolic and flavonoid contents - DPPH and H2O2 scavenging assays were positively correlated. The Invitro Antioxidant results were also correlated with the Insilico method using xanthine oxidase (XOD) and superoxide dismutase (SOD) as targets. Microscopic and physicochemical parameters are valuable for authenticating drug identification and purity. G. bilamellata is a potential perspective source of antioxidants. Keywords: Grewia bilamellata Gagnep, Antioxidant, Pharmacognostic, Insilico
REVIEW OF SERINE PROTEASE INHIBITORS: DEVELOPMENT AND APPLICATIONS / مراجعة الادبيات المتعلقة بمثبطات انزيم السيرين بروتياز من حيث التطوير والاستخدامات
قطاع الدراسات الصيدلية / العقاقير
تخصص البحث : العقاقير
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 20
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ مي رمضان - مؤلف رئيسي
العقاقير
A class of enzymes known as proteases is responsible for catalyzing the breakdown of covalent peptide bonds. Proteases account for almost 2% of the genes in humans, infectious organisms, and other forms of life. Serine proteases, so named because they have a nucleophilic serine residue at the active site, make for nearly one-third of all proteases. Although digesting is their primary role in humans, they also play roles in immunological response, apoptosis, inflammation, blood coagulation, and signal transduction. They are therefore very attractive biological targets for the design of pharmacological molecules that can modulate their activity. This review presents a description of the catalytic activity and structure of serine proteases, and the different strategies to develop inhibitors. Serine protease inhibitors targeting thrombin, FXa, and human neutrophil elastase (HNE), and dipeptidylprotease-4 (DPP4) will be described. It includes therapeutically applied, and those drugs which are still under investigation in clinical trials in the last two decades. Keywords: Serine protease inhibitor, Thrombin, FXa, HNE, DPP4
PHYTOCHEMICAL VARIABILITY IN THE ESSENTIAL OIL OF MATRICARIA PUBESCENS from ALGERIA / الاختلاف الكيميائي النباتي للزيت العطري نبات ماتريكاريا بوبيسنس من الجزائر
قطاع الدراسات الصيدلية / العقاقير
تخصص البحث : العقاقير
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 12
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ محمد طاهر بن موسى - مؤلف رئيسي
العقاقير
Matricaria pubescens is a plant belonging to the asteraceae family naturally found in Algeria, as well as throughout all of North Africa, commonly used as folk medicine to treat several diseases under several preparations, the essential oil obtained from the aerial parts of the plant contains chemical components, that were determined using GC-MS, the variability in the levels of these components was studied in nine samples collected on different dates from two sites in the northern Sahara of Algeria. 58 components were determined, with different levels, the constituents: Delta.3-Carene, Beta-Ocimene, Allo-Ocimene, (-)-Bornyl acetate, Aromadendrene 2, 4.10(14)-Muuroladien-8 beta-ol, Valencene 2 and Dihydronepetalactone II are shown to be the main chemical components of the essential oil, from the analytical obtained restults, the statistical analysis with HCA and PCA tests have revealed different chemical patterns. Aside from seasonality, chemotypes may be the most important source of variation, and the existence of several varieties featured by different chemical distribution patterns, this outcome should be confirmed by further studies with higher samples. Keywords: Essential Oil, Variability, Matricaria Pubescens
EVALUATION OF ANTIOXIDANT, ANTIBACTERIAL, ANTALGIC AND ANTI-INFLAMMATORY PROPERTIES OF CRATAEGUS AZAROLUS / تقييم الخصائص المضادة للأكسدة و البيكتيريا والألم والالتهابات لنبات Crataegus azarolus
قطاع الدراسات الصيدلية / العقاقير
تخصص البحث : العقاقير
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 14
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ زينب لكاش - مؤلف رئيسي
العقاقير
The purpose of this study was to examine the potential effects of a Crataegus azarolus extract on antioxidant, antibacterial, analgesic, and anti-inflammatory activities. Additionally, a phytochemical analysis was conducted to identify the chemical constituents of the extract. The antioxidant potential was assessed using DPPH, FRAP, and ?-carotene assays, while the antibacterial activity was evaluated against five different microorganisms by measuring the diameter of inhibition zones. The anti-inflammatory and analgesic effects were examined by inducing paw edema with carrageenan and intraperitoneal injection of acetic acid, respectively. The results indicated that the extract exhibited strong antioxidant activity, and the highest antibacterial activity was observed against Bacillus subtilis, with an inhibition zone diameter of 18 mm. Additionally, administering 400 mg/kg of the extract resulted in a strong inhibition of paw edema, showing a percentage reduction of 42.28% compared to the control group. Similarly, the methanolic extract (600 mg/kg) of Crataegus azarolus showed inhibition of pain caused by acetic acid in mice. The findings of the study propose that the extract has the potential to provide bioactive compounds, and that its individual constituents may act together in a synergistic manner. Therefore, Crataegus azarolus extract could be used as an alternative to synthetic anti-inflammatory and analgesic compounds in the pharmaceutical industries in the future. Keywords: Crataegus azarolus, antioxidant, antibacterial, antalgic, anti-inflammatory
PHYTOCHEMICALS AND BIOACTIVITIES OF CEDRUS LIBANI A. RICH / المكونات الفعالة و الفعالية الحيوية لنبات الأرز اللبناني Cedrus libani A. Rich
قطاع الدراسات الصيدلية / العقاقير
تخصص البحث : العقاقير
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 17
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ ريم نيال - مؤلف رئيسي
العقاقير
Cedrus libani (Pinaceae) is a species native to the Mediterranean area. It was traditionally used to cure a wide range of illnesses, including inflammatory and respiratory conditions, as well as to relieve toothache pain. The purpose of this review is to deepen our understanding of both the composition and the bioactivities of this plant by providing a survey of the information that is currently available on numerous electronic databases about botanical aspects, traditional uses, phytochemistry, and biological activities. This review demonstrated that various plant parts have a wide range of bioactivities and active phytochemicals, suggesting that they might be used as a source of natural antioxidants and antimicrobials for industrial and medical applications. Human blood-related illnesses may also be treated with Cedrus libani. Moreover, it exhibits promising analgesic and anti-inflammatory properties. However, more toxicological and clinical research is required to determine its applications’ safety and efficacy in treating human ailments. Keywords: Cedrus libani, Essential oil, Tar, Phytochemistry, Biological activities
IN VITRO PROPAGATION OF ALGERIAN LAVANDULA STOECHAS AND ASSESSMENT OF BIOCHEMICAL COMPOSITION AND THEIR ANTIOXIDANT ACTIVITY / الإكثار المخبري لنبات Lavandula stoechas الجزائري وتقييم التركيب الكيميائي الحيوي ونشاطه كمضاد للأكسدة
قطاع الدراسات الصيدلية / العقاقير
تخصص البحث : العقاقير
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 13
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ رقية بن يامي - مؤلف رئيسي
العقاقير
The present research aimed to investigate the effect of plant growth regulators (PGRs) on the in vitro proliferation of Lavandula stoechas shoots and assessment of the antioxidant potential and total phenolic content of extracts obtained from in vitro cultures and wild plants of L. stoechas, as well as, the chemical analysis of the phenolic content of both extracts carried out using HPLC-DAD. The optimal shoot proliferation and biomass accumulation were achieved with the combination of 0.5 mg/l 6-benzyladenine (BA) and 0.5 mg/l gibberellic acid (GA3). The hydroalcoholic extract obtained from in vitro cultures contained the highest amount of phenolic compounds (83.18 mg GAE/g DW extract) compared to the total phenolic content of wild plants extract (32.33 mg GAE/g DW extract). Furthermore, the hydroalcoholic extract from in vitro cultures exhibited a higher antiradical effect against 2,2-diphenyl-1-picrylhydrazyl (DPPH) with IC50 value of 51.38 ± 0.7µg/ml and higher antioxidant capacity (757.26 mg GAE/g DW) observed by total antioxidant capacity (TAC) assay. The chemical profile of both extracts revealed the presence of naringenin, acacetin, rhamnetin, luteolin 3’- 7 diglucoside, and hesperetin. Overall, the results suggest that in vitro cultures of L. stoechas could serve as a potential source for producing active metabolites with high antioxidant properties. Keywords: Micropropagation, Phenolic compounds, free radical scavenging, Lavender, HPLC-DAD
EVALUATION OF ANTIOXIDANT ACTIVITIES OF SOME ABUNDANT MARINE MACROALGAE, EGYPT / تقييم أنشطة كسح مضادات الأكسدة لبعض الطحالب البحرية الوفيرة، مصر
قطاع الدراسات الصيدلية / العقاقير
تخصص البحث : العقاقير
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 13
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ هاجر كمال أحمد - مؤلف رئيسي
العقاقير
This study investigated the antioxidant scavenging activity of nine marine macroalgae extracts collected from different Egyptian coasts. The extracts were tested using the DPPH assay to exhibit various antioxidant activities. All extracts showed lower DPPH free radical scavenging compared to ascorbic acid. Antioxidant effects increased with extract concentration, with brown algae exhibiting the highest activity among green and red algae. Specifically, the brown alga Polycladia myrica extract was most effective against DPPH free radicals, while the green alga Ulva fasciata demonstrated the lowest scavenging effect. At a concentration of 1000ug/ml, the most effective species against DPPH free radicals were brown macroalgae (Polycladia myrica > Turbinaria turbinata > Hormophysa cuneiformis), followed by red macroalgae (Jania rubens > Hypnea cornuta > Galaxura rugosa), whereas the green macroalgae showed the lowest scavenging effect (Caulerpa racemosa > Halimeda tuna > Ulva fasciata). The IC50 values indicated that Ulva fasciata, Halimeda tuna, and Caulerpa racemosa had the highest antioxidant activity, then red algae Galaxura rugosa> Hypnea cornuta> Jania rubens while brown algae Hormophysa cuneiformis, Turbinaria turbinata, and Polycladia myrica recorded the lowest values. Overall, the studied macroalgae species demonstrated significant antioxidant potential due to bioactive compounds like polyphenols, saponins, and tannins. Caulerpa racemosa exhibited the highest polyphenol concentration, while Galaxura rugosa had the lowest. Ulva fasciata, Hormophysa cuneiformis and Polycladia myrica showed the highest total saponins content, while Hypnea cornuta registered the highest total tannins content. These findings suggest that macroalgae could serve as dietary alternatives due to their strong antioxidant capabilities. Keywords: Abundant marine macroalgae, Egyptian coasts, Ascorbic acid, Antioxidant activity, DPPH scavenging
INFLUENCES OF ENVIRONMENTAL FACTORS ON THE ACTIVE METABOLITES AND CERTAIN BIOACTIVITIES OF DESERT DATE LEAF AND FRUIT (BALANITES AEGYPTIACA L. DELILE) GROWN IN EGYPT / تاثيرات العوامل البيئية علي المواد الفعالة و التاثيرات البيولوجية لورقة وثمرة نبات بلح الصحراء النامي في مصر
قطاع الدراسات الصيدلية / العقاقير
تخصص البحث : العقاقير
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 15
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ أحمد محمد زاهر - مؤلف رئيسي
العقاقير
Balanites aegyptiaca (L.) Delile is a medicinal wild tree, naturally distributed in wide regions of Africa and South Asia. The fruits are commercially available in Egypt as antidiabetic natural products. The current study aimed to determine the effects of environmental factors on the metabolites and the bioactivities of fruit and leaf extracts. The main ecological differences between the sites of collection, Wadi El?Gemal and Baris Oasis, are temperature, aridity, humidity, and location. The fruit extract of Baris Oasis showed higher saponin content and saponin metabolites (LC?MS analysis) than the fruit extract of Wadi El?Gemal. Baris Oasis fruit extract showed twice the inhibition of ??Glucosidase than Wadi El?Gemal fruit extract. Twelve phenolics and flavonoids were detected in Baris Oasis leaf extract, while ten compounds were detected in Wadi El?Gemal leaf extract using HPLC. The higher quality and quantity of flavonoids and phenolics of Baris Oasis leaf extract than Wadi El?Gemal leaf extract, have significant effects on the antioxidant and acetylcholinesterase inhibitory activities. In conclusion, elevation of temperature and aridity have significant positive effects on the synthesis and accumulation of B. aegyptiaca saponins, phenolics and flavonoids. The bioactivities of the fruit and leaf extracts are directly proportional to the variation in metabolites due to abiotic stresses. Key word: Balanites aegyptiaca L, Ecological Factors, Saponins, Flavonoids, Phenolics.
AROMATHERAPY APROPOS OF NORMAL FLORA: FRIEND OR FOE? / العلاج العطري فى مقابل الميكروبيوم: صديق أم عدو؟
قطاع الدراسات الصيدلية / العقاقير
تخصص البحث : العقاقير
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 12
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ هبة يحيى - مؤلف رئيسي
العقاقير
Aromatherapy was practiced since prehistory for alleviating diseases and/or stress relief. Recently, it bloomed after complementary and alternative medicine became popular among individuals seeking homeopathic treatment to evade side effects. Studies revealed that aromatherapy owes its efficacy to complex essential oils that show qualitative and quantitative variabilities depending on plant-related factors. Aromatherapy is delivered through inhalation and dermal contact where volatile oils act locally or penetrate to the circulation to exert systemic effect. Many essential oils possess antibacterial, antifungal, antiviral and/or cytotoxic activities which raises concern against their random use and believing they are completely safe. Also, little evidence exists depicting the selectivity of essential oils towards pathogenic rather than endogenous microbiota. Relevantly, microflora dysbiosis can negatively impact the host’s health. Therefore, this article aims to emphasize that long term studies are required to regulate aromatherapy practice without endangering the body’s homeostasis and refute the misleading assumption of its unquestionable safety. Keywords: Aromatherapy; Complementary and alternative medicine (CAM); Microbiota; Essential oils (EO); Dysbiosis
BIOSYNTHESIS AND ANTIBACTERIAL EVALUATION OF ZINC OXIDE NANOPARTICLES from ONION EXTRACT (ALLIUM CEPA) / التخليق الحيوي لجسيمات أكسيد الزنك النانوية بواسطة مستخلص البصل الأحمر (Allium cepa) والنشاط المضاد للبكتيريا
قطاع الدراسات الصيدلية / الكيمياء الدوائية
تخصص البحث : الكيمياء الدوائية
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 13
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ جمال عبدالعزيز جودة - مؤلف رئيسي
الكيمياء الدوائية
The aim of this research is to use red onion extract (Allium cepa) in the green synthesis of zinc oxide nanoparticles (ZnO NPs). With an average crystal size of 8.13 nm, X-ray diffraction (XRD) scanning was used to determine the hexagonal wurtzite structure of the ZnO NPs. Infrared spectra (FT-IR) provided evidence of capping and biogenesis stability of ZnO NPs. The absorption peak at 374 nm with an energy gap of 3.32 eV was determined by surface plasmon resonance via UV-visible analysis. Morphological analysis was performed, and the results demonstrated the formation of spherical ZnO NPs with sizes ranging from 2.83 to 15.35 nm. The great purity, intensity, and crystal width of zinc and oxygen was determined by EDS. When compared to a common antibiotic such as ciprofloxacin, which was used as a positive control in the inhibitory zone, ZnO NPs showed more antibacterial effect against E. coli than S. aureus bacteria. Onion extract and DMSO were added to the control wells, but these additions did not produce an inhibitory zone that could be seen. Keywords: Green synthesis, Nanoparticles, TEM, EDX, Antimicrobial activity
A VALIDATED RP-HPLC METHOD FOR SEPARATION AND DETERMINATION OF ETORICOXIB AND PARACETAMOL IN BULK AND PHARMACEUTICAL DOSAGE FORM / طريقة موثقة لفصل وتعيين الأتيروكسيب والباراسيتامول في المستحضرات الصيدلية المختلفة
قطاع الدراسات الصيدلية / الكيمياء التحليلية الصيدلية
تخصص البحث : الكيمياء التحليلية الصيدلية
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 05/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 14
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ لمى غزال - مؤلف رئيسي
الكيمياء التحليلية الصيدلية
Etoricoxib is a non-steroidal, anti-inflammatory drug (NSAID) and a selective inhibitor of Cyclooxygenase 2 (COX2), which makes it safer on the gastrointestinal tract than the other NSAIDs. Given the importance of Etoricoxib’s, its combination with paracetamol offers higher efficacy and fewer side effects. In addition to the popularity of this combination in the pharmaceutical industry and the diversity of its manufacturing companies, this study aims to develop a novel, precise, selective, rapid and economic assay method for the simultaneous quantitative determination of Etoricoxib and paracetamol in bulk and pharmaceutical dosage form using reverse phase HPLC. This developed method has a short run time of less than 4 minutes using a C18 column (150×4.6) mm 5?m, a mobile phase of methanol and ammonium acetate (pH =3.5) in a ratio (60:40), respectively. The elution was observed at 245 nm using a PDA detector; the retention times of paracetamol and Etoricoxib were found to be 2.493 and 3.64 min, respectively, and a resolution factor larger than 2. Linearity was established with correlation coefficient values of 0.9991, 0.9994 for both Etoricoxib and paracetamol drugs. Precision was within the relative standard deviation of less than 2% for both drugs, and the percentage recoveries were found to be 99.98% and 99.35% for paracetamol and Etoricoxib, respectively. LOD and LOQ of paracetamol were 1.4 ?g/ml and 4.3 ?g/ml, respectively, and 0.52 ?g/ml and 1.6 ?g/ml for Etoricoxib, respectively. The selectivity test results showed no interference from the tablet excipients during the separation process, which verifies that this method is easily applicable to quality control labs and pharmaceutical industries, in addition to being fast, accurate and cost-effective. Keywords: Etoricoxib, RP-HPLC, assay, validation, paracetamol, simultaneous
QUALITY ASSESSMENT OF HERBAL MEDICINAL PRODUCTS IN BENIGN PROSTATE HYPERTROPHY: CHEMICAL MARKERS AND FINGERPRINT ANALYSIS / تقييم جودة المستحضرات النباتية المستخدمة لعلاج ضخامة البروستات الحميد: تحليل الواسمات الكيميائية والبصمة الكروماتوغرافية
قطاع الدراسات الصيدلية / الكيمياء التحليلية الصيدلية
تخصص البحث : الكيمياء التحليلية الصيدلية
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 06/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 14
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ هدى مندو - مؤلف رئيسي
الكيمياء التحليلية الصيدلية
Herbal products with beneficial influence on BPH have been widely used. These herbal preparations include the extracts of Serenoa repens and/or Pumpkin seed oil. Chemical markers are crucial in the quality control of herbal medicines and construct with chromatographic fingerprint a powerful tool for quality assessment. The aim of this study was to evaluate the quality of herbal formulations used to treat BPH in Syrian local market by identifying fatty acids profile and tocopherols, besides analyzing fingerprint of methanol extracts. For this purpose, GC/MS analysis has been applied. The fatty acids content was consistent with the quality requirements in four samples. ? and ? tocopherols content was in agreement of previous studies between 181-875 µg/g with the exception of one sample reached 77.5 µg/g for both ? and ? tocopherol. chromatographic fingerprint revealed the shortage of ?7 phytosterols in pumpkin seed oil samples and the presence of parabens in one of them. The absence of Luaric acid in one sample was confirmed with chromatographic fingerprint. The results have highlighted on the importance of combining active markers with chromatographic fingerprint as a suitable technique for quality evaluation of herbal products as well efficiency and safety. Keywords: BPH; herbal products; GC/MS; official markers; analytical markers; chromatographic fingerprint
BLACK FUNGUS WHAT DO WE KNOW ABOUT IT? KNOWLEDGE, ATTITUDE AND PRACTICE OF HEALTH CARE PROFESSIONALS IN IRAQ / الفطر الاسود ماذا نعرف عنه؟ المعرفة والسلوك والممارسة لدى مقدمي الرعاية الصحية في العراق
قطاع الدراسات الصيدلية / الصيدلة الأكلينيكية
تخصص البحث : الصيدلة الأكلينيكية
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 06/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 12
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ لمى مؤيد العبيدي - مؤلف رئيسي
الصيدلة الأكلينيكية
Background: Black fungus is a serious infectious disease caused by mucoromycetes. The first cases of black fungus among patients with COVID-19 were reported in India in December 2020, followed by new cases in other countries like Iraq. This study aimed to assess the knowledge, attitude, and practice of healthcare professionals (HCPs) toward black fungus. Methods: This was a cross-sectional study, in which HCPs who are members of the Iraqi syndicate of physicians, dentists, and pharmacists from different Iraqi cities participated in this online survey between the 11th of September and the 11th of November 2021. Results: In total 340 HCPs participated in this study. 55% of participating HCPs had a poor level of knowledge. 52% of participating HCPs thought that COVID-19 vaccination could protect the person from black fungus disease. 44% of the participating HCPs thought that black fungus can be prevented in COVID-19 patients through the wise use of steroids and good glycemic control in diabetic patients. 96% of HCPs thought that the best management plan for black fungus includes antifungal therapy plus surgery. Conclusions: Although the majority of HCPs have general information about black fungus, the level of knowledge regarding different aspects of the disease is poor. Iraqi HCPs required more comprehensive educational programs to increase their knowledge of black fungus and other infectious diseases. Keywords: Black fungus; COVID-19; Knowledge; Attitude; Practice
KNOWLEDGE AND PERCEPTIONS OF FINAL-YEAR STUDENTS TOWARDS PHARMACOVIGILANCE AND ADVERSE DRUG REACTION REPORTING AT THE FACULTY OF MEDICAL SCIENCES, AL-RAZI UNIVERSITY - SANA`A - YEMEN / معارف ومواقف طلاب السنة النهائية تجاه التيقظ الدوائي والإبلاغ عن التأثيرات الجانبيَّة للأدوية في كلية العلوم الطبية ، جامعة الرازي - صنعاء - اليمن
قطاع الدراسات الصيدلية / الصيدلة الأكلينيكية
تخصص البحث : الصيدلة الأكلينيكية
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 06/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 9
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ نبيل البصير - مؤلف رئيسي
الصيدلة الأكلينيكية
Background: There is a serious problem with adverse drug reactions (ADRs) everywhere including Yemen. Since it helps with the detection, assessment, reporting and prevention of ADRs, pharmacovigilance (PV) is an essential part of the healthcare system. The unbiased reporting of ADRs remains the foundation of PV. Students majoring in healthcare should acquire the knowledge and skills necessary to conduct PV in a range of clinical settings. The primary objective of this study was to evaluate the understanding and attitudes of final-year Pharmacy, Nursing, and Midwifery students at Al-Razi University in Sana’a, Yemen regarding PV and ADRs reporting. Methods: The study followed descriptive cross-sectional approach. A validated, self-administered questionnaire with three parts—demographic information, knowledge, and perceptions of Pharmacovigilance was online distributed to final-year Pharmacy, Nursing, and Midwifery students. The questionnaire was given to 175 students; 122 of them responded with a percentage (69.7%). Results: The majority of respondents were male (79.5%). More than the tow-third of the students 68.9% were beyond the age of 23. Although the majority of students 80% heard about the terms of ADRs and PV, but only 50% and 57.4% of the respondents respectively, could define the both terms correctly. However, only 11.48 % of them, nevertheless, took a PV course. More than a half of them (56.6%) had a positive perceptions towards pharmacovigilance and ADR reporting and had a moderate degree of knowledge (68.9%). Conclusion: The study demonstrated that the participants lacked sufficient knowledge of pharmacovigilance and ADR reporting. They showed a moderate level of understanding of reporting ADRs as well as a favorable opinion of dealing with and reporting ADRs. Yemen’s health care curriculum should include lessons on pharmacovigilance Keywords: Adverse Drug Reaction Reporting; Pharmacovigilance Knowledge; Healthcare Students, Perception, Yemen
EXPLORING THE ROLE OF COMMUNITY PHARMACISTS IN DRUG INTERACTIONS: A CROSS-SECTIONAL STUDY IN PALESTINE / استكشاف دور صيادلة المجتمع في التداخلات الدوائية: دراسة مقطعية في فلسطين
قطاع الدراسات الصيدلية / الصيدلة الأكلينيكية
تخصص البحث : الصيدلة الأكلينيكية
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 06/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 13
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ عبد الله رباع - مؤلف رئيسي
الصيدلة الأكلينيكية
Objective: This study aims to evaluate community pharmacists’ knowledge, attitudes, and practices about drug-drug interactions (DDIs) and drug-food/herbs interactions (DFIs) in Palestine. Method: A cross-sectional study using a self-administered questionnaire including thirty questions was conducted among community pharmacists to assess the pharmacists’ knowledge, attitudes, and practices related to DDIs and DFIs. The t-test, ANOVA, and chi-square tests were used to find the associations with characteristics and demographics. The data were analyzed using SPSS version 22. Results: A total of 361 participants completed the questionnaire; most participants (49.6%) have bachelor’s degrees in pharmacy and 26.0% have a doctor of pharmacy degree. 20.8% of participants have been a pharmacist for ten years or longer, while 10.2% have been a pharmacists from 7 to 9 years. Pharmacists’ DIs knowledge level was acceptable; their DFIs knowledge was higher than their DDIs knowledge. Pharmacists with long experience working in chain pharmacies with internet services significantly had better knowledge about DDI and DFI. Pharmacists’ attitude toward the importance of recognizing DIs was positive, whereas their practice reflected less positivity. Conclusion: Community pharmacists in Palestine have acceptable knowledge of drug interactions. However, their DFIs knowledge is higher than their DDIs knowledge, and they have favorable attitudes toward DIs, whereas their practice was negative. However, community pharmacists need to improve their knowledge of DDIs and DFIs and stay current with new drug interactions. Keywords: Drug-drug interaction, Drug-herbs interaction, Pharmacists Knowledge, Palestine
CAPECITABINE EFFICACY WITH ADJUVANT RADIOTHERAPY IN THE TREATMENT OF EARLY STAGE BREAST CANCER (RETROSPECTIVE STUDY) / تاثيـر عقـار الكابيسيتابيــن مـع العــلاج الإشعاعي المساعــد فـي عــلاج مرضـى سرطــان الثـدي المبكـر ( دراسـة مرجعيـة)
قطاع الدراسات الصيدلية / الصيدلة الأكلينيكية
تخصص البحث : الصيدلة الأكلينيكية
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 06/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 13
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ رحاب محمد - مؤلف رئيسي
الصيدلة الأكلينيكية
Objectives: Our goal is to assess the effectiveness and toxicity of capecitabine as radio-sensatizer with adjuvant radiotherapy in breast cancer (BC) patients. Methods: On the radiation days, the patients got capecitabine 825 mg/m2 every 12 hours with a 25% dosage reduction if creatinine clearance was lower than 30 ml/min with radiation therapy of dose of 4240 cGy administered in 16 fractions at a rate of 2.65 Gy each over the course of three weeks. In addition, a boost dose of 14 Gy administered in 7 fractions was added to the lumpectomy bed. Results: Study group were evaluated at 3, 12, 24, 36, 48, and 60 months. At 3 and 12 months, 29 patients (61.7%) exhibited G1 skin hyperpigmentation. At 60-month, 3 patients (7.5%) had G2 lymphedema (p = 0.226). At 24th month, 4 patients (9.3%) had G1 telangiectasia, 5 patients (11.6%) developed G1 skin fibrosis (p = 0.001). Moreover, one patient (2.3%) experienced a G1 cardiac event (p = 0.416). At 3 month radiation pneumonitis G1 was identified in 3 patients (6.4%) (p = 0.416). Despite the fact that 6 patients (15%) developed distant metastases, there were no instances of local recurrence. the OS rate was 85.1%. Conclusion: Adjuvant radiation combined with capecitabine is well tolerated and effective Key words: Radio-sensitizer, capecitabine, efficiency, breast cancer
INVESTIGATING THE GENETIC EFFECT OF VKORC1 GENE POLYMORPHISM ON WARFARIN RESPONSE IN EGYPTIAN HEART PATIENTS / دراسة التأثير الجيني لتعدد الأنماط الجيني VKORC1 على استجابة الوارفارين لدى مرضى القلب المصريين
قطاع الدراسات الصيدلية / الصيدلة الأكلينيكية
تخصص البحث : الصيدلة الأكلينيكية
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 06/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 12
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ محمد محمود محمد عبداللطيف - مؤلف رئيسي
الصيدلة الأكلينيكية
Warfarin is a common oral anticoagulant, but the dose needed for each patient can vary by as much as 20 times due to both environmental and genetic factors. Some of the most important genetic targets of warfarin include the vitamin K epoxide reductase complex 1 (VKORC1) gene. This study investigated the genetic effect of the VKORC1 gene on warfarin response using real-time PCR in a total of 100 warfarin-treated Egyptian heart patients and 40 controls. This study confirmed a genetic association of the VKORC1 SNP rs9934438 with warfarin response. The VKORC1 SNP analysis identified the presence of 26% homozygous variant (A/A), 42% heterozygous variant (A/G), and 32% wild-type variant (G/G) among patients compared to 20%, 25%, and 55% of controls. A significant difference was found between VKORC1 genotype allelic distribution in study patients and controls (P<0.05). Patients carriers of the VKORC1 genotype (A/G) required a lower daily warfarin dose than the other variants (P=0.006). The total number of patients who reached the optimal therapeutic goal of warfarin dose of 100 study patients was 19 compared to 81 ones who did not. Females required lower daily warfarin doses than males. VKORC1 gene polymorphism did not affect INR outcomes, but it was associated with a decrease in warfarin response. The VKORC1 gene polymorphism could explain the interindividual variation in warfarin response among Egyptian patients. Our findings suggest that dosing algorithms incorporating the genetic elements of the VKORC1 genotype are essential to determine warfarin dose and could optimize the therapeutic effectiveness of warfarin and minimize its adverse effects. Keywords: Warfarin, Anticoagulation, Pharmacogenomics, VKORC1, Genotyping, Egyptians
EVALUATION OF THE PHARMACISTS KNOWLEDGE, AWARENESS AND PRACTICES TOWARDS PHARMACOVIGILANCE IN SYRIA / تقييم اليقظة الدوائية لدى الصيدلي في سورية
قطاع الدراسات الصيدلية / الصيدلة الأكلينيكية
تخصص البحث : الصيدلة الأكلينيكية
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 06/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 8
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ معاذ العجلاني - مؤلف رئيسي
الصيدلة الأكلينيكية
Background: The system of pharmacovigilance in Syria is administered by the Syrian Ministry Of Health (MOH), it is responsible for receiving reports from the patient all over the country for possible Adverse Drug Reactions (ADRs). Pharmacists are members of this system and play a very important role in its implementation. Until now, Few pharmacists know about this system in Syria, how to deal with it, or even how important it is. Aims: To discover Syrian pharmacists’ knowledge, awareness, proceedings, and possible obstacles encountered while reporting the (ADRs). and to estimate the sociodemographic data from Damascus and rural Damascus. Methods: We used a self-administered, cross-sectional, questionnaire-based survey conducted on a random sample of 235 registered pharmacists in 2 Syrian governorates. Results: 84% was the rate of response, 45% had a familiarity with the PV system in Syria but the rest didn’t. Some of them have received complaints from patients about unknown side effects, and their percentage was 60%, only 22% reported it. The most significant percentage of notifications was through the company’s representative (10%), and 6% used the Ministry of Health (MOH) form. Conclusions: Pharmacists who participated in the survey showed limited knowledge about pharmacovigilance (PV), also about its importance and extent of application in Syria. They had overlapping attitudes toward the reports, although they emphasized the importance of it. However, the current level of participation is rather low. Keywords: Pharmacovigilance, Pharmacists, Survey, Syria, Adverse Drug Reaction
PORTULACA OLERACEA LEAVES EXTRACT ATTENUATES CYCLOPHOSPHAMIDE TOXICITY IN MICE / يخفف مستخلص أوراق بورتولاسا اوليراسي ( الرجلة) من سمية السيكلوفوسفاميد في الفئران
قطاع الدراسات الصيدلية / الكيمياء الحيوية
تخصص البحث : الكيمياء الحيوية
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 06/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 10
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ أميرة محمد صالح - مؤلف رئيسي
الكيمياء الحيوية
Cyclophosphamide (CTX) is a well-known broad-spectrum anticancer agent however; it has severe side effects on vital organs. Portulaca oleracea that belongs to family Portulacaceae showed promising nutritional and biomedical properties. This research aimed to estimate the effect of P. oleracea leaves extract (POLE) on CTX toxicity in male CD-1 mice. Total phenolic, flavonoids contents, total antioxidant capacity (TAC), and DPPH scavenging activity (DPPH %) were determined in POLE by phytochemical quantitative analysis. Thirty-six male CD-1 mice were divided into three groups (n=12), as negative control, CTX (200 mg/kg) injected, and CTX/POLE (200 mg/kg) injected groups. Blood samples, liver tissues and kidney tissues were collected for hematological, biochemical and histopathological investigations. The total phenolic, flavonoid content, saponin, DPPH %, and total antioxidant capacity (TAC) were 1368 µg/ml, 302 µg/ml, 0.1250 µg/g, 65%, 193 µg/ml, respectively. Treatment with POLE augmented the hematological, biochemical, and histopathological changes induced by CTX of hepatic and renal tissues. Collectively, POLE reduced the hepato-renal toxicities caused by CTX in mice by improving the antioxidants/oxidants hemostasis Keywords: Portulaca oleracea leaves, Portulacaceae, Antioxidants, Phytochemicals, Cyclophosphamide, Hepato-renal, Toxicity
CORRELATION BETWEEN HIGH SENSITIVITY C-REACTIVE PROTEIN AND LIPID PROFILE IN YOUNG PATIENTS WITH DIABETES MELLITUS TYPE 1 IN HOMS-SYRIA / العلاقة بين البروتين المتفاعل C عالي الحساسية والصّيغة الليبيدية عند اليافعين المصابين بداء السّكري من النّمط الأول في حمص – سوريا
قطاع الدراسات الصيدلية / الكيمياء الحيوية
تخصص البحث : الكيمياء الحيوية
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 06/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 8
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ ريهام نعيم ديبان - مؤلف رئيسي
الكيمياء الحيوية
In this study, we evaluated levels of high sensitivity C-reactive protein (hsCRP), as a predictor marker of cardiovascular disease, and lipid profile and studied the relationship between them in young patients with diabetes mellitus type 1 (DMT1). This study was conducted on 71 young with (DMT1), at Al-Arman Healthy Center in Homs- Syria, compared to 25 healthy controls at the same age. Blood samples were collected, and laboratory measurements were performed which included: HbA1c, FBG, TG, LDL-c, HDL-c, TC, hsCRP. Depending on the (HbA1c) values, all study patients had uncontrolled DM, and 58.5% of them had dyslipidemia. DMT1 patients had significantly higher serum concentrations of hsCRP, which indicates the presence of low-grade chronic inflammation. Significant positive correlation was found between (hsCRP) and (HbA1c, TG, LDL-c, TC), and a significant negative correlation with (HDL-c) in DMT 1 group. Conclusions, Atherosclerosis and vascular changes begin from an early age in DMT 1 patients with poor glycaemic control. Key words: Type 1 diabetes, atherosclerosis, cardiovascular disease, hsCRP.
RAG1: THE CORNERSTONE GENE INVOLVED IN VARIOUS IMMUNE DISEASES AND CANCERS / الجين المحفز لاعادة الارتباط1 حجر الزاوية فى امراض مناعية عديدة وسرطانات
قطاع الدراسات الصيدلية / الكيمياء الحيوية
تخصص البحث : الكيمياء الحيوية
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 06/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 18
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ مصطفى فتحى - مؤلف رئيسي
الكيمياء الحيوية
RAG1 is an abbreviation for recombination activating gene-1 which is the cornerstone in the process of V(D)J recombination. The process enables our immune system to produce an unlimited number of antibodies and T-cell receptors to fight almost any infection. RAG1 mutations are greatly involved in the development of primary immunodeficiencies (PID) including the early-onset severe combined immunodeficiency and Omenn Syndrome. Additionally, RAG mutations are found in delayed-onset cases of combined immunodeficiency with autoimmunity and/or granulomas. The early-onset category occurs due to severe immunodeficiency and the delayed-onset one occurs due to immune dysregulation. Furthermore, RAG1 abnormalities are found in several hematological neoplasms such as acute lymphoblastic leukemia (ALL), chronic myeloid leukemia (CML), myelodysplastic syndrome (MDS) and lymphoma. The mechanism by which RAG1 contributes to different types of cancer is not fully understood. In this review, we display the role of RAG1 in the different diseases digging to understand how it works and whether it can be a promising biomarker for the progression of some of these conditions. Keywords: RAG1; V(D)J recombination; Mutations; Immunodeficiencies; Leukemia
DISTURBANCES IN SOME PLASMA AMINO ACIDS METABOLOMIC PROFILES AND THEIR DERIVATIVES IN EGYPTIAN OBESE WOMEN / الاضطرابات في بعض الملامح الأيضية للأحماض الأمينية ومشتقاتها في البلازما في السيدات المصريات البدينات
قطاع الدراسات الصيدلية / الكيمياء الحيوية
تخصص البحث : الكيمياء الحيوية
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 06/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 18
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ عبد الرحمن عبد القوي - مؤلف رئيسي
الكيمياء الحيوية
Background: Obesity is a risk factor for several lifestyle diseases such as type II diabetes, hypertension, cancer and others. Studies have shown that plasma amino acids profile may be useful for screening lifestyle diseases. This case-control study aimed at investigating disturbances in acidic, basic, neutral, sulfur-containing amino acids besides imino amino acids profiles in Egyptian obese women to reach a healthy body and to stop any future complications of overweight. Methods: A total of 85 women were enrolled in this study that were classified into 5 groups (17 persons each):1- healthy, 2-overweights, 3-moderate obese, 4-severe obese, 5-morbid obese. Plasma metabolomic profile of the previous amino acids was evaluated using amino acid analyzer. Results: A statistically significant ascending increase in the mean plasma levels of citrulline, glutamate, proline and cysteine together with significant stepwise decrease in ornithine, serine, threonine, aspartate, histidine, arginine, methionine & taurine in obese subgroups as compared to controls. Conclusion: Our results of multivariate regression analysis demonstrated that the significantly elevated circulating lysine or proline levels were the best independent predictors for recognizing obese patients at risk of associated metabolic disorders and that the plasma AA metabolomic profile can be used as important biomarkers for early prediction of overweight women. Amino acids-directed regimens intervention could have significant impact on obesity management. Keywords: chromatography; Overweight; Obesity-subgroups; Amino acids metabolomic profile; Prediction
DISTURBANCES IN PLASMA ALIPHATIC AND AROMATIC AMINO ACIDS METABOLOMIC PROFILES IN EGYPTIAN OBESE WOMEN / الاضطرابات في ملامح التمثيل الغذائي للأحماض الأمينية الأليفاتية والأروماتيه في البلازما في السيدات المصريات البدينات
قطاع الدراسات الصيدلية / الكيمياء الحيوية
تخصص البحث : الكيمياء الحيوية
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 06/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 17
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ عبد الرحمن عبد القوي - مؤلف رئيسي
الكيمياء الحيوية
Background: According to the World Health Organization (WHO), Egypt ranks 18th with the highest prevalence of obesity worldwide. The impact of obesity is a result of its comorbidities rather than a direct effect. Therefore, it is of utmost importance to investigate its risk factors to establish its potential preventive strategies for future complications. This study aimed at investigating the disturbances in aliphatic and aromatic amino acids profiles in Egyptian obese women. Methods: The study comprised 85 women that were classified into 5 groups (17 each):1- healthy, 2-overweights, 3-moderate obese, 4-severe obese, 5-morbid obese. Plasma metabolomic profile of the previous amino acids was evaluated using an amino acid analyzer. Results: Compared with controls, obese subgroups had significantly stepwise higher levels in the mean plasma levels of tyrosine, total aromatic amino acids (AAA) and alanine together with significantly lower levels of leucine, isoleucine, total branched chain amino acids (BCAA), BCAA/AAA ratio and glycine. Conclusion: Multivariate regression analysis indicated that the significantly elevated circulating alanine levels were good independent predictors for identifying obese patients at risk of associated metabolic disorders. Moreover, the study suggested that AA metabolomic profile can be used as useful tools for early prediction of overweight women. Amino acids-directed regimens intervention could serve as a specific potential targeting strategy for management of obesity. Keywords: Overweight; Obesity-subgroups; Aliphatic and aromatic AA profile; prediction; chromatography
TM6SF2 RS58542926 IS LINKED TO THROMBOCYTOPENIA-RELATED ADVANCED HEPATIC FIBROSIS IN EGYPTIAN PATIENTS WITH chrONIC HEPATITIS C / المتغير rs58542926 في جين عبر الغشاء 6 من أفراد العائلة الفائقة 2 مرتبط بقلة الصفائح الدموية في مرضي التليف الكبدي الشديد المصريين الناتج عن الإلتهاب الكبدي الفيروسي سي المزمن
قطاع الدراسات الصيدلية / الكيمياء الحيوية
تخصص البحث : الكيمياء الحيوية
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 06/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 11
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ خالد ثابت - مؤلف رئيسي
الكيمياء الحيوية
Thrombocytopenia in chronic hepatitis C (CHC) patients is a serious complication, which hinders starting and continuation of several invasive diagnoses and treatment protocols. This problem is multifactorial; therefore, it is important to explore host factors linked to it. Interestingly while we were investigating the association between rs58542926 polymorphism in TM6SF2 gene and both hepatic & extra hepatic changes, we found a significant association between TM6SF2 risk allele and thrombocytopenia in Egyptian CHC patients. There are 351 participants in this study. We measured gene expression of IL-6 and TNF-? in peripheral blood mononuclear cells (PBMCs) and plasma thrombopoietin (TPO) level in 20 CHC patients with advanced fibrosis. The minor allele was linked to elevated thrombocytopenia (TCP), and lower TPO levels. These findings raise concerns regarding the effect of this polymorphism on different diagnostic techniques and the outcomes of hepatitis C virus (HCV) treatment. In conclusion, we demonstrated for the first time that the occurrence of the minor allele of the rs58542926 polymorphism is associated not only with hepatic but also with extrahepatic (thrombocytopenia) alterations in CHC Egyptian patients, allowing for the use of this variant as a pharmacogenetic marker to recognize CHC patients who have a high possibility for developing late stages of liver fibrosis and extrahepatic complications. Key words: TM6SF2; rs58542926; hepatitis C, thrombocytopenia
ASSOCIATION OF SERUM URIC ACID WITH METABOLIC SYNDROME COMPONENTS IN SYRIAN POPULATION / ارتباط حمض البول المصلي بمكونات المتلازمة الاستقلابية عند السكان السوريين
قطاع الدراسات الصيدلية / الكيمياء الحيوية
تخصص البحث : الكيمياء الحيوية
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 06/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 10
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ غفران حميد - مؤلف رئيسي
الكيمياء الحيوية
The current study aimed to investigate the correlation between serum uric acid levels and metabolic syndrome and its components in adult patients from Homs, Syria. This study included 86 subjects divided into two groups; 20 healthy controls and 66 patients with metabolic syndrome. Physical examination and blood pressure (BP) information were recorded. Serum uric acid (SUA), fasting blood glucose (FBG), total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were tested. As a result, subjects with metabolic syndrome had significantly higher levels of SUA than controls (P < 0.001). Metabolic syndrome patients with hyperuricemia had higher BMI, WC, TC and LDL-C than those with normal uric acid levels (P < 0.05). Moreover, subjects with more metabolic syndrome components had higher SUA levels. On the other hand, SUA was positively correlated with WC, DBP, FBG, TG, TC and LDL-C and negatively with HDL-C (P < 0.05). Therefore, in general, elevated SUA levels appear to be positively associated with metabolic syndrome components in Syrian population and can be considered a therapeutic target in controlling metabolic syndrome disorders. Keywords: Metabolic syndrome components; Hyperuricemia; Serum uric acid
ESTIMATING EXPOSURE TO SARS-COV-2 AT THE ARAB INTERNATIONAL UNIVERSITY, SYRIA: A SNAPSHOT AFTER A YEAR OF PANDEMIC / تقدير التعرّض ل?يروس SARS-CoV-2 في الجامعة العربية الدولية، سورية: لقطةٌ بعد عامٍ من الجائحة
قطاع الدراسات الصيدلية / الميكروبيولوجيا والمناعة
تخصص البحث : الميكروبيولوجيا والمناعة
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 07/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 8
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ وفاء الحبّال - مؤلف رئيسي
الميكروبيولوجيا والمناعة
Background: After a year of pandemic in Syria, how prevalent the seropositivity is, and how far we are from herd immunity is a raised question. Aim: Our study aimed to estimate the overall exposure to SARS-COV-2 depending on the COVID-19-specific symptoms as well as the seroprevalence of anti-SARS-CoV-2 IgG antibodies among students and staff at the Arab International University (AIU) in Syria. Methods: All AIU students and staff were invited to voluntarily participate in the study by filling out and submitting an anonymous web-based survey. Responses were evaluated then asymptomatic and pauci-symptomatic respondents were notified of a phlebotomy appointment. Anti-SARS-CoV-2 IgG antibodies were analyzed by ELISA. Results: One third (122 of 381; 32%) of respondents were asymptomatic. The remaining respondents reported COVID-19-specific symptoms (177 of 381; 46.5%), mainly anosmia and/or ageusia (132 of 177; 74.5%), or nonspecific symptoms (82 of 381; 21.5%). Frequencies of symptoms onset showed a strong, positive correlation with the incidence of confirmed SARS-CoV-2 RNA-positive cases reported by the Ministry of Health in Syria. Anti-SARS-CoV-2 IgG antibodies were similarly detected in both symptomatic (21 of 33; 63.6%) and asymptomatic (39 of 61; 63.9%) individuals (P=.97). Conclusions: After a year of pandemic, a 69% exposure to SARS-CoV-2 is roughly estimated amongst asymptomatic and pauci-symptomatic individuals. Further post-vaccination assessment of seroprevalence against SARS-CoV-2 in future large-scale studies might provide a more complete picture of the SARS-CoV-2 epidemic in Syria. Keywords: COVID-19, anti-SARS-CoV-2 IgG, asymptomatic, seroprevalence, exposure, Syria
THYME AND CUMIN NANOEMULSION AS A PROMISING ANTIMICROBIAL AGENT AGAINST MULTIDRUG-RESISTANT STAPHYLOCOCCUS AUREUS / مستحلب نانوي الزعتر والكمون كعامل واعد مضاد للميكروبات ضد المكورات العنقودية الذهبية المقاومة للأدوية المتعددة
قطاع الدراسات الصيدلية / الميكروبيولوجيا والمناعة
تخصص البحث : الميكروبيولوجيا والمناعة
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 15
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ وداد أحمد - مؤلف رئيسي
الميكروبيولوجيا والمناعة
This study aimed to compare thyme, cumin extracts, and their nanoemulsion as antibacterial on S.aureus. The methods include , preparation of aqueous, oil extract and nanoemulsion from thyme and cumin, determination of MIC using the resazurin microdilution method, detection of mecA and coagulase genes by PCR before and after the treatment with nanoemulsions, figuring the bacteria after treatment using TEM. the result showed that the MIC values of thymus oil 100% , thymus oil nano emulsion 20% , cumin oil 100% ,cumin oil nanoemulsion 20% ,mix oil 20% ,thymus oil 20% ,cumin oil 20% ( 0.048, 0.156 , 1.562 ,10 , 0.625 , 0.312 , 10 ,) mg/m respectively , mecA and coagulase genes was absent after the in vitro treatment and TEM microscope showed the bacteria with extensive damage to the cell membrane. the result showed that nanoemulsion has a higher effect on S. aureus than essential oil alone Keywords: Thymus, cumin, nanoparticle, antibacterial activity, TEM microscope
ANTIMICROBIAL AND IN VITRO CYTOTOXICITY EFFECT OF GREEN SYNTHESIZED SILVER NANOPARTICLES USING AQUEOUS LEAF EXTRACT OF SENNA HIRSUTA / تأثير السمية الخلوية و التأثير المضاد للميكروبات فى المختبر لجسيمات الفضة النانوية الخضراء المركبة باستخدام مستخلص الأوراق المائية من سينا هيرسوتا
قطاع الدراسات الصيدلية / الميكروبيولوجيا والمناعة
تخصص البحث : الميكروبيولوجيا والمناعة
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 11
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ ناراهاري ن. بالي - مؤلف رئيسي
الميكروبيولوجيا والمناعة
In this study, we prepared silver nanoparticles (AgNPs) using an aqueous Senna hirsuta leaf extract. The AgNPs were characterized using UV visible spectrophotometer, particle size, zeta potential, energy diffraction X-ray (EDAX), Fourier transform infrared (FTIR), transmission electron microscopy (TEM) and determined antibacterial and cytotoxicity impact of the AgNPs. UV-Visible spectroscopy of Senna hirsuta leaf extract synthesized into AgNPs was carried out at various time intervals. The peak (? max) was observed at 436–446 nm and it was broad. The particle size of the synthesized AgNPs was found 28.3 nm. The Zeta potential of AgNPs was found -4.4 mV. TEM analysis demonstrated that nanoparticles have a spherical form. According to the EDAX analysis, the extract effectively capped the AgNPs. Zone of inhibition of AgNPs were observed more than those of the extract. Silver nanoparticles demonstrated effective antimicrobial activity due to their large surface area, which allows for stronger contact with cell wall of bacteria. In vitro cytotoxicity showed that synthesized AgNPs a prominent cytotoxicity activity against MCF7 cell lines. Keywords: Green synthesis, Senna hirsuta, Silver nanoparticles, Antimicrobial activity, Cytotoxicity
CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF ALLIUM SATIVUM L. ESSENTIAL OIL AGAINST MDR BACTERIA ISOLATED from BUCCAL CAVITY AFFECTED BY CARIES / عنوان التركيب الكيميائي والنشاط البيولوجي للزيت الأساسي من نبات الثوم ضد بكتيريا ذات المقاومة المتعددة للأدوية معزولة من تجويف الفم المتأثر بالتسوس
قطاع الدراسات الصيدلية / الميكروبيولوجيا والمناعة
تخصص البحث : الميكروبيولوجيا والمناعة
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 12
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ سلس سيدي محمد عمار - مؤلف رئيسي
الميكروبيولوجيا والمناعة
Natural remedies have proven to be very effective and have fewer side effects than commercial antibiotics. The objectives of this work were to determine the chemical compounds and biological (antibacterial and antioxidant) activities of Allium sativum L. essential oil against three multi-drug strains of bacteria isolated from buccal cavity affected by caries namely Aerococcus viridans, Staphylococcus epidermidis, and S. xylosus. The essential oil was extracted by hydrodistillation, chemical compounds were quantified by gas chromatography-mass spectrometry and gas chromatography with flame ionization detection. 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing power were used to study antioxidant activity. Antibacterial activity was carried out by the disk diffusion method. The average yield of the essential oil was 0.173±0.009% (w/w). All tested strains were resistant to three different classes of antibiotics. Diallyl disulfide (39.22%) and diallyl trisulfide (34.85%) were the main components of Allium sativum L. essential oils. DPPH radical scavenging assay showed the median inhibitory concentration (IC50) value of 51.12±11.77 mg/ml, while the ferric reducing power assay recorded the median effective concentration (EC50) value of 6.54 ± 0.63 mg/ml. The results showed that all multidrug-resistant bacteria strains tested are sensitive to essential oils. The results indicate that Allium sativum L. essential oil exercises good in-vitro antibacterial and weak to moderate in-vitro antioxidant activities. Keywords: Allium sativum L., essential oil, antibacterial activity, antioxidant activity, multidrug-resistant bacteria
THE EFFECT OF ESSENTIAL OILS OF selectED PLANTS ON CLINICAL ISOLATES OF CANDIDA SPECIES GROWTH, TRANSITION AND BIOFILM FORMATION / عنوان تأثير الزيوت الطياره لنباتات مختارة على نموعزلات اكلينيكية من المبيضات والانتقال وتشكيل الاغشيه الحيويه
قطاع الدراسات الصيدلية / الميكروبيولوجيا والمناعة
تخصص البحث : الميكروبيولوجيا والمناعة
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 24
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ الاء جلال أمين جلال - مؤلف رئيسي
الميكروبيولوجيا والمناعة
Candida species are one of common opportunistic pathogens in humans. Detection of antifungal resistance between candida species become increasing, a great interest in creating new antifungals utilizing natural sources like plant essential oils (EOs) . This study aimed to evaluate the effect of EOs of selected plants on Candida species growth, transition, and biofilm formation as well on the expression levels of certain virulence genes. Patients and methods: Fifty candida isolates were collected from napkin dermatitis and oral candidiasis patients, different Candida species were identified phenotypically. Essential oils for some medicinal plants (basil, clove, garlic, and peppermint) were extracted by hydro-distillation. Their antifungal activity was assessed by minimal inhibitory concentration methods. In addition the effect of EOs on different Candida ultrastructure was detected by scanning electron microscope, the antibiofilm characteristics were examined by microtiter plate method. The ability of tested EOs to interfere with transition of candida from blastopore to hyphae form was evaluated. The effect of basil and clove oils on various virulent genes was determined by analyzing the expression levels of HWP, ALS3, SAP3 genes by RT-PCR. Results: C. albicans was the most prevalent species. For all tested Candida isolates, the EOs displayed a high antifungal activity. The EOs decreased the Candida transition, disrupted the Candida ultrastructure and suppressed their ability of biofilm formation, clove oil exposure significantly downregulated the expression of HWP1, ALS3 and SAP3 genes, in addition basil oil downregulated the expression of ALS3 and SAP3.Conclusion: This study concluded that clove oil is the best essential oil among other EOs that inhibited growth, transition and biofilm formation by lowest MIC and downregulated HWP1, AlS3 and SAP3 genes, which suggest their potential application for its use as antifungal therapy. Keywords: Essential oil; C. albicans; Bio?lm; HWP1; ALS3; SAP3 genes
ANALYSIS OF BACTERIAL COMMUNITY AND ITS BIODEGRADATION ABILITY OF 17-? ESTRADIOL IN WASTEWATER PLANT IN ASSIUT, UPPER EGYPT / تحليل المجتمع البكتيري وقدرته على التحلل الحيوي لمادة 17- بيتا استراديول في محطة مياه الصرف الصحي في أسيوط ، صعيد مصر
قطاع الدراسات الصيدلية / الميكروبيولوجيا والمناعة
تخصص البحث : الميكروبيولوجيا والمناعة
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 16
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ هبة أحمد محمد - مؤلف رئيسي
الميكروبيولوجيا والمناعة
Wastewater treatment is essential for environmental sanitation in urban environments. Nevertheless, chemical, and organic substances, and microorganisms accumulate in wastewater treatment plants (WWTPs), which may be liberated into the environment via their effluents. This study aimed to analyze the bacterial community at the sewage treatment plant entrance in Assiut, Upper Egypt as well as their degradation ability of 17-? estradiol (E2) as an Isolates were identified by conventional methods and, 16S rRNA gene sequencing. E2 degradation by different strains was assessed using HPLC/UV. Conventional methods and 16S rRNA based amplicon sequencing revealed three bacteria including Bordetella sputigena (ASc1), Pigmentiphaga daeguensis (ASc3), and Stenotrophomonas pavanii (ASc4), and when tested for E2 degradation, they degraded 65.5%, 59%, and 66.4% of E2 (50 mg. L-1) respectively. Other bacterial strains were identified using 16S rRNA based amplicon sequencing. Bacteroidetes, Firmicutes and Proteobacteria were the prominent phyla. However, Actinobacteria, Cyanobacteria, Chloroflexi, Caldithrix, and Chlamydiae were detected in lower proportions. Keywords: Bacterial community composition; Wastewater treatment plants (WWTPs); 17-? estradiol (E2); Biodegradation
IDENTICAL ANTIMICROBIAL EFFECT OF CEFTRIAXONE AND CEFOTAXIME AMONG DIFFERENT BACTERIAL ISOLATES / التأثير المتطابق لمضادات الميكروبات للسيفترياكسون والسيفوتاكسيم بين العزلات البكتيرية المختلفة
قطاع الدراسات الصيدلية / الميكروبيولوجيا والمناعة
تخصص البحث : الميكروبيولوجيا والمناعة
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 10
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ خالد أبو العيش - مؤلف رئيسي
الميكروبيولوجيا والمناعة
Background: This study aimed to screen the antimicrobial-resistant profile of these 3rd generation cephalosporins and to identify their similarity and interchangeability. Method: This cross-sectional study, was conducted in all the government hospitals across the Gaza Strip, Palestine. The study started in November 2017 and continued till December 2022. All clinical samples such as wound swabs (pus), urine, sputum, blood, cerebrospinal fluid (CSF), stool, and others in which ceftriaxone and cefotaxime were examined at the same time were collected from hospitalized patients and outpatient clinic attendants. After the identification of the bacterial isolates, a standard disc diffusion technique for drug susceptibility tests was performed. This study was reviewed and approved by The Palestinian Helsinki Committee. Results: In the current study, 24,120 isolates in which ceftriaxone and cefotaxime were examined at the same time were studied. The predominant organisms isolated were, Escherichia coli 9,720 (40.3%), Klebsiella spp. 5,497 (22.8%), Pseudomonas spp. 2,630 (10.9%), and Staphylococcus aureus 1941 (8.0%). Bacterial isolates showed 57.1% and 57.8% resistance against ceftriaxone and cefotaxime, respectively. In this study, 22,404 (92.9%) bacterial isolates were with identical susceptibility test results to ceftriaxone and cefotaxime. The highest match of susceptibility was seen in Acinetobacter spp. 97.3% (770/791), Klebsiella spp. 95.3% (5,241/5,491), and Escherichia coli 94.9% (9,220/9,720). In Staphylococcus aureus it was 90.6% (1,758/1,941). Conclusion: Ceftriaxone and cefotaxime can be interchangeable in most Enterobacteriaceae bacterial species. Ongoing surveillance of different bacterial antimicrobial resistance and multidrug resistance is strongly recommended together with the consideration of implementing antibiotics stewardship programs in all hospitals. Keywords: Bacterial isolates, bacterial resistance, cefotaxime, ceftriaxone, multidrug resistance
POTENTIAL OF ETHANOL EXTRACT OF GUAVA (PSIDIUM GUAJAVA L.) LEAVES AS ADJUVANT-ANTIBIOTIC ON SALMONELLA TYPHI charACTERIZED / امكانية فعالية مستخلص الإيثانول لأوراق الجوافة (بسيديم جوافة ل.) كمضاد حيوي مساعد على مرض السالمونيلا التيفية
قطاع الدراسات الصيدلية / الميكروبيولوجيا والمناعة
تخصص البحث : الميكروبيولوجيا والمناعة
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 10
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ واهيو هندرارتي - مؤلف رئيسي
الميكروبيولوجيا والمناعة
Guava (Psidium guajava L.) contains many chemical compounds, including quercetin, myricetin, epicatechin, and tannins which have been shown to have efficacy as an efflux pump inhibitor (EPI) in several pathogenic bacteria. This study aimed to investigate the synergistic effect of the ethanol extract of guava leaves with five common antibiotics on Salmonella typhi isolates expressing active efflux pumps. Bacterial characterization was carried out on clinical isolates of S. typhi, and molecular resistance mechanisms were determined using qRT-PCR by measuring the expression of the Acriflavine resistance protein B (AcrB) efflux pump. Guava leaves were extracted by maceration using 70% ethanol. Tests were carried out using the diffusion method with tetracycline, ampicillin, trimethoprim-sulfamethoxazole, chloramphenicol, and ciprofloxacin. The extract was made in serial concentrations of 0.01, 0.025, 0.05, 0.075, and 0.1%. The results showed that the tested isolates decreased the expression of AcrB from 11.48167 to 7.38818 ?g/mL after adding CCCP. The synergistic effect occurred at (1) extract concentration of 0.1% against tetracycline 30 ?g, (2) concentration of 0.05% against ampicillin10?g, (3) concentration of 0.075% against trimethoprim-sulfamethoxazole 23.75-1.25 ?g, (4) concentration of 0.05% against chloramphenicol 30 ?g, (5) concentration of 0.025% against ciprofloxacin 5 µg. The ethanol extract of guava leaves (Psidium guajava L.) synergized with the studied antibiotics and displayed efflux pump inhibitory activity. Consequently, it could serve as a natural antibiotic adjuvant candidate. Keywords: Antibiotics, efflux pump inhibitor, guava leaves, Salmonella thypi ST019
OPTIMIZATION OF KOJIC ACID PRODUCTION BY ASPERGILLUS COCULTURE AND IT’S APPLICATION AS ANTI-BROWNING AGENT / تحسين إنتاج حامض الكوجيك المتبلور من فطرة الأسبرجيللس وتطبيقة كعامل مضاد للتلون
قطاع الدراسات الصيدلية / الميكروبيولوجيا والمناعة
تخصص البحث : الميكروبيولوجيا والمناعة
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 15
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ غادة عبدالمنصف محمود - مؤلف رئيسي
الميكروبيولوجيا والمناعة
Kojic acid has applications in many fields so it is important to increase the amount of kojic acid. In this study production of kojic acid increased using coculture of Aspergillus oryzae ASU44 (OL314732) and A. flavus ASU45 (OL314748) than single culture, after optimizing by Box-Behnken statistical design, the production enhanced to 114.28 g/l using Glucose (150, g/l) (A), Yeast extract (5 g/l) (B), KH2PO4 (3 g/l) (C), MgSO4.7H2O (0.5 g/l) (D) and pH (3) (E). The design was effective and applicable with coefficient (R2) 99.1% and adjusted R2 value 98.1%. Ethyl acetate was the best extraction solvent with ratio 1:1 and the crystals of kojic acid appeared in needle shape after evaporation of ethyl acetate using rotatory evaporator. The crystalized kojic acid has the ability to inhibit the fungal pigment of A. niger ASU311. By increasing the KA concentration, the black color decreased, especially at 10 µg/ml comparing with the control samples. On the other hand, crystalized KA preserved the color of apple juice from the conversion to brown color and the activity increased by increasing the concentration until 50 µg/ml for 6 hrs comparing with the control. Application of kojic acid produced naturally using Aspergillus coculture considered safe and environmentally friendly for increasing the freshness of the fruit juice. Keywords: Co-culture. Fungi. Experimental design. Decolorization
THE THERAPEUTIC POTENTIAL OF COLD ATMOSPHERIC PLASMA AGAINST PATHOGENIC BACTERIA INHABITING DIABETIC WOUNDS / الفعالية العلاجية لبلازما الهواء الجوي غير الحرارية ضد البكتيريا الممرضة التي تستعمر جروح السكري
قطاع الدراسات الصيدلية / الميكروبيولوجيا والمناعة
تخصص البحث : الميكروبيولوجيا والمناعة
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 16
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ جمال بدر - مؤلف رئيسي
الميكروبيولوجيا والمناعة
Impaired healing process of diabetic wounds is a common problem among diabetic patients. These diabetic wounds are slow to heal and can lead to serious complications if left untreated. One of the reasons diabetic wounds are slow to heal is that pathogenic bacteria often colonize them. Cold atmospheric plasma (CAP) is a promising new technology that has been shown to effectively kill and inhibit pathogenic bacteria in diabetic wounds. In the current study, we examined by what means CAP works and its potential impact as a diabetic wound treatment. Fifty diabetic patients aged from 20 to 60 years old with diabetic wounds were included in our study using the swab method. We used general and specific culture media to isolate the pathogenic bacteria from diabetic wounds and identified the bacteria via different techniques using a light microscope (oil lens) and VITEK. Moreover, we confirmed the identification of isolated bacteria by 16S rRNA. Our results demonstrated the direct anti-bacterial effect of CAP against the isolated bacteria. The most prominent bacterial type in diabetic wounds was Staphylococcus sp. CAP demonstrated the highest effect against Bacillus sp. and Paenibacillus sp., which were responsible for relevant changes in the skin and were associated with the skin immune response and microbiota. Our results revealed the therapeutic use of CAP as a powerful strategy to overcome the invading pathogenic bacteria that colonize diabetic wounds and hence improve the healing process of diabetic wounds. Keywords: Bacterial colonization; cold atmospheric plasma; diabetic wounds; Paenibacillus species, pathogenic bacteria
SPIRONOLACTONE ALTERS THE LEVELS OF NEURONAL FUNCTION MARKERS IN AUTISTIC RATS / عقار سبيرونولاكتون يغير مستويات علامات الوظيفة العصبية في الجرذان المصابة بالتوحد
قطاع الدراسات الصيدلية / علم الأدوية والسموم
تخصص البحث : علم الأدوية والسموم
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 12
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ بوبش شارما - مؤلف رئيسي
علم الأدوية والسموم
Autism spectrum disorder (ASD) is a neurodevelopmental disorder that is characterized by repetitive behavior and impairment in social behavior. ASD is a complex disorder with complex etiology that involves multiple genes, environmental factors, and epigenetic mechanism. Many clinical and pre-clinical study have demonstrated the association of propionic acid (PPA) with ASD. This study evaluates the potential effect of spironolactone in PPA induced ASD phenotype. PPA (250 mg/kg, po) was administered to Albino Wistar rats from post-natal day (PND) 21st to 23rd to induce ASD like neurobehavioral and neurobiochemical alterations. Animals were experimented for behavioral (elevated plus maze, three chambered social interaction apparatus, and Y-maze apparatus), biochemical parameters (BDNF, synapsin II), and blood-brain barrier impairment. Post-natal PPA exposure resulted impairment in social behavior, anxiety, and repetitive behavior in animals. Furthermore, PPA exposure caused reduction in the levels of BDNF and synapsin II in rats’ brain. Spironolactone (25 mg/kg and 50 mg/kg) administration was observed to ameliorate post-natal PPA exposed behavioral and biochemical impairments in animals. Keywords: Propionic acid, autism, spironolactone, social behavior, repetitive behavior, BDNF, Synapsin
EFFECT OF COLOCASIA ESCULENTA (L.) SCHOTT (ARACEAE) ETHANOL LEAF EXTRACT ON SPERM CONCENTRATION IN MALE WISTAR RATS / تأثير مستخلص أوراق الإيثانول لنبات كولوكاسيا اسكيولنتا (ال.) شوت (العائلة النخلية) على تركيز الحيوانات المنوية في ذكور فئران ويستار
قطاع الدراسات الصيدلية / علم الأدوية والسموم
تخصص البحث : علم الأدوية والسموم
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 12
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ تيميتايو أو أجايي - مؤلف رئيسي
علم الأدوية والسموم
Colocasia esculenta (L.) Schott (Araceae) is a staple food which supply needed nutrients in Africa and is therefore, employed in ethnomedicine. This study aimed to evaluate the effect of the leaf ethanol extract as well as its partitioned fractions (ethyl acetate, butanol and aqueous) on some male reproductive parameters such as sperm count, motility and morphology as well as the histology of testes in male Wistar rats after 15 days of administration of the extracts/fractions. The ethanol extract showed significant increases of 66 and 213% in sperm concentration at 500 and 1000 mg/kg respectively. This activity was largely retained in the ethylacetate with 200, 213 and 300% in sperm concentration at 50, 100 and 200 mg/kg, respectively and aqueous fractions with 274 and 657% increase at 50 and 100 mg/kg, respectively. Motility and morphology were not significantly altered. The histology of the tests revealed that spermatozoa cells were the positively affected cells. Keywords: Histology, motility, morphology, fractions
AMELIORATED EFFECT OF COMBINED TREATMENT OF BLUEBERRY FRUIT EXTRACT AND VOGLIBOSE ON STREPTOZOTOCIN-INDUCED TYPE ? DIABETES MELLITUS IN RATS / التأثير المحسن للعلاج المتزامن لمستخلص فاكهة التوت والفوجليبوز في الجرذان المستحدث فيها داء السكري بالستربتوزوتوسين
قطاع الدراسات الصيدلية / علم الأدوية والسموم
تخصص البحث : علم الأدوية والسموم
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 15
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ أمنية بهيج - مؤلف رئيسي
علم الأدوية والسموم
Blueberries are rich in anthocyanins, which have been studied for many years. Interest in these compounds has grown attributing to their possible therapeutic and beneficial effects. Therefore, this study aimed to investigate the potential antioxidant, anti-inflammatory, hepatoprotective, anti-diabetic, and anti-inflammatory effects of blueberry fruit extract supplement (25% anthocyanin) in combination with aloglibtin and voglibose in streptozotocin (STZ)-induced diabetic rats. Diabetes mellitus was induced in six groups with a single 45 mg/kg STZ injection. However, 5 of these groups were treated with alogliptin (Alog), voglibose (Vog), blueberry fruit extract (BB-fruit extract), combined treatment of (Alog + BB-fruit extract), and (Vog + BB-fruit extract), respectively, for three weeks. Serum glucose, C-peptide, lipid profile, liver enzymes, kidney enzymes, and TNF-? levels were estimated. Meanwhile, alpha-glucosidase, catalase, and superoxide dismutase (SOD) activities and malondialdehyde (MDA) levels were determined in rats’ liver tissues. All diabetic groups unveiled a significant decrease in serum glucose and C-peptide level after administering BB-fruit extract. Combination of BB-fruit extract and vog is effective in achieving sufficient glycemic control by significantly reducing inflammation, oxidative stress, hyperlipidemia, and hyperglycemia. These findings imply that this combination is an effective strategy for controlling diabetes. Keywords: Type ? diabetes; Vaccinium uliginosum L.; Berries; Liver; Antioxidant
THE POTENTIAL PROTECTIVE EFFECT OF STEVIA REBAUDIANA ON DEXAMETHASONE-INDUCED NONALCOHOLIC FATTY LIVER DISEASE IN RATS: ROLE OF PPAR-? / التأثير الوقائي المحتمل لمستخلص نبات ستيفيا على مرض الكبد الدهني غير الكحولي الناجم عن استخدام عقارالديكساميثازون في الجرذان: دور PPAR-?
قطاع الدراسات الصيدلية / علم الأدوية والسموم
تخصص البحث : علم الأدوية والسموم
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 19
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ إيمان شحاتة - مؤلف رئيسي
علم الأدوية والسموم
Background: Currently, using medicinal herbs has a great interest in the treatment of nonalcoholic fatty liver disease (NAFLD). This study evaluated the impact of stevia on the hallmark signs of dexamethasone-induced NAFLD in rats. Methods: group I: Negative control rats received 2 ml of vehicle carboxy methyl cellulose (CMC). group II: Rats received dexamethasone 8 mg/kg/day intraperitoneal for 6 days. group III: Rats received oral stevia extract 400 mg/kg/day for 12 days. group IV: Rats received dexamethasone + stevia extract. Serum ALT, AST, TC, TG and adiponectin were measured. MDA, GPx and TNF-? were estimated in liver homogenate. Histopathological changes and PPAR-? expressions in the liver were evaluated. Results: Dexamethasone caused a reduction in GPx and ADP levels with an elevation in ALT, AST, TC, TG, MDA and TNF-?. There was a decrease in immunostaining expression of liver PPAR-?. Stevia caused an improvement in liver enzymes, lipid profile, oxidative stress, TNF-? and adiponectin levels. Conclusion: Stevia provided some protection against dexamethasone-induced NAFLD. Keywords: Dexamethasone, NAFLD, PPAR-? and Stevia
COMPARATIVE ASSESSMENT OF THE PROTECTIVE EFFECT OF N-ACETYL CYSTEINE AND CAPTOPRIL AGAINST CYCLOPHOSPHAMIDE-INDUCED NEPHROTOXICITY IN WISTAR RATS / تقييم مقارن للتأثير الوقائي لـ ان اسيتايل سيستايين و كابتوبريل ضد السمية الكلوية التي يسببها سيكلوفوسفاميد في جرذان ويستار
قطاع الدراسات الصيدلية / علم الأدوية والسموم
تخصص البحث : علم الأدوية والسموم
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 15
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ ولاء إبراهيم محمد - مؤلف رئيسي
علم الأدوية والسموم
This work was executed to assess and compare the protective effect of n-acetyl cysteine (NAC) and captopril (CPL) in the nephrotoxicity induced by cyclophosphamide (CYP) in rats. Thirty-six adult male Wistar rats were separated into six groups (6 rats each). The control group received normal saline. NAC and CPL treated groups received NAC (200 mg/kg) and CPL (60 mg/kg) respectively for six consecutive days. Whereas CYP treated group received CYP (150 mg/kg) on the sixth day of the experiment. NAC+CYP and CPL+CYP treated groups received NAC and CPL respectively for 6 days then administered a single dose of CYP on the sixth day of the experiment. The intraperitoneal route is the method of administration of all drugs. CYP treated group showed a significant rise in levels of serum urea, creatinine, uric acid, and cystatin C, as also kidney MDA and IL-6 levels. Furthermore, it showed a significant decrease in kidney SOD, GSH, and IL-10 levels. Furthermore, the level of the Bcl2 gene was downregulated. Histopathological changes in the kidney exhibited marked tubular degenerative changes. Additionally, there was a significant increase in caspase-3 within the epithelium of the renal tubules. On the other hand, NAC+CYP and CPL+CYP treated groups protected against abnormal biochemical and histological changes and restored the Bcl2 gene. These data suggested that the administration of NAC and CPL could protect against CYP- induced nephrotoxic effect through their antioxidant, anti-inflammatory and anti-apoptotic effects. Keywords: Cyclophosphamide, N-acetyl cysteine, Captopril, Oxidative stress, Gene expression, Anti-inflammatory, Nephrotoxicity
METFORMIN AND CARVEDILOL AMELIORATE ISOPRENALINE-INDUCED MYOCARDIAL INFARCTION IN NON-DIABETIC RATS THROUGH AMPK ACTIVATION AND SUPPRESSION OF APOPTOSIS / الميتفورمين و الكارفيديلول يحسنان احتشاء عضلة القلب الناجم عن الأيزوبرينالين في الجرذان غير المصابة بالسكري من خلال تنشيط AMPK وقمع موت الخلايا المبرمج
قطاع الدراسات الصيدلية / علم الأدوية والسموم
تخصص البحث : علم الأدوية والسموم
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 19
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ إيمان محمد علي - مؤلف رئيسي
علم الأدوية والسموم
The objective of this study was to clarify the ameliorative effect of metformin (MET), carvedilol (CAR), and their combination and the underlying mechanisms in a non-diabetic rat model of isoprenaline (ISO)-induced MI. The adult male Wistar rats were allocated into six groups (n=8). Control group A received saline while control group B received DMSO 0.5% (i.p) for 10 days. ISO-treated group: received ISO (85 mg/kg.i.p.) on the first and second day of the experiment with the injection of normal saline for 10 days from the first day. ISO+MET and ISO+CAR-treated groups: received ISO as previously described and MET (200 mg/kg.i.p.) and CAR (10 mg/kg.i.p.) respectively for 10 days from the first day of the experiment. ISO+MET+CAR-treated group: received ISO, MET, and CAR as previously described. In the ISO group, the rise in serum cardiac biomarkers cTn-I and LDH provided evidence of MI. In addition, cardiac MDA, IL6, caspase-3, and Bax gene levels were significantly elevated, while cardiac SOD, GSH, pAMPK, eNOS, Bcl-2 gene, and Bcl-2/Bax ratio levels were significantly reduced with histopathological changes in cardiac tissue. Whereas posttreatment with MET, CAR, and their combination significantly reversed these overwhelming ISO-induced damaging effects on the heart. In conclusion, MET, CAR, and their combination could improve myocardial injury in ISO-induced MI through AMPK signaling pathway activation, and anti-apoptotic, anti-inflammatory, and antioxidant mechanisms. Subsequently, promote the recovery of cardiomyocyte function. Keywords: Metformin, Carvedilol, Myocardial infarction, AMPK, Apoptosis, Gene expression
COMPARISON EFFECT OF DIFFERENT COMBINATIONS OF METFORMIN, ATORVASTATIN, CAPTOPRIL AND ASPIRIN ON OXIDATIVE STRESS MARKERS OF GASTRIC AND LIVER TISSUES OF DIABETIC RATS / مقارنة تأثير تركيبات مختلفة من الميتفورمين والأتورفاستاتين والكابتوبريل والأسبرين على علامات الإجهاد التأكسدي لأنسجة المعدة والكبد لدى الجرذان المصابة بالسكري
قطاع الدراسات الصيدلية / علم الأدوية والسموم
تخصص البحث : علم الأدوية والسموم
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 10
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ مريم باسيبان - مؤلف رئيسي
علم الأدوية والسموم
Some recent studies have been suggested high - dose aspirin consumption in diabetic patient because of its modifying effect on metabolism of glucose and lipid. In other side many researches showed aspirin induced gastrointestinal damage. Investigation of the effect of high-dose aspirin consumption in combinations with metformin, captopril and atorvastatin on oxidative stress markers in the stomach and liver tissues of diabetic rats. Rats divided into eleven groups: control (Cont), diabetic (D), and 9 treated groups with various combination of metformin (M), atorvastatin (AT), captopril (C), and aspirin (ASA). Animals were treated orally by M, C, AT and ASA daily for 6 weeks. Finally, oxidative markers were evaluated in stomach and liver tissues. The Malondialdehyde (MDA) level significantly boosted while total thiol content remarkably decreased in the diabetic group than the control. Administration of the different combinations of C, M, AT and ASA could significantly attenuate above parameters. Combination of the metformin, aspirin, atorvastatin and captopril has more marked effects on oxidative stress reduction in the stomach and liver tissues of diabetic rats and could ameliorated probably oxidative stress induced by aspirin. Keywords: Diabetes, Stomach, Liver, Aspirin, Drugs combination
HYPERHOMOCYSTEINEMIA: ITS IMPACT ON CARDIOVASCULAR DISEASE AND ATHEROSCLEROSIS / فرط الهوموسستئين في الدم: تأثيره على أمراض القلب والأوعية الدموية وتصلب الشرايين
قطاع الدراسات الصيدلية / علم الأدوية والسموم
تخصص البحث : علم الأدوية والسموم
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 21
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ إسراء جلال - مؤلف رئيسي
علم الأدوية والسموم
Hyperhomocysteinemia (HHcy) was found to be a risk factor for cardiovascular disorders and many other diseases. HHcy occurs when homocysteine (Hcy) exceeds 15 ?mol/L. The prevalence of HHcy ranges from 5 – 10% in the general adult population. HHcy can cause atherosclerosis and heart dysfunction through oxidative stress, inflammation, endothelial interaction with leukocytes, coagulation, endothelial fibrosis, SMCs proliferation, apoptosis, lipids dysregulation, epigenetic modifications and modifications of electrical conduction in the heart. Some of the drugs that have been studied have been shown to be effective in reducing the level of HHcy. Conclusion: High Hcy level can be a strong cause in several disorders including cardiovascular diseases. So, serious effort should be made to avoid and treat HHcy. All evidence suggests that Hcy is causally connected to atherosclerosis by many mechanisms. But future studies should concentrate on the molecular pathways of HHcy in cardiac dysfunction, and the effects of Hcy-lowering medications on cardiac dysfunction. Keywords: Homocysteine, hyperhomocysteinemia, cardiac dysfunction, atherosclerosis, ER stress and oxidative stress
LUNG HISTO-PATHOLOGICAL CHANGES AND SODS GENE EXPRESSION IN WISTER RATS EXPOSED TO CONVENTIONAL AND ELECTRONIC CIGARETTES / التغيرات في الأنسجة الرئوية والتعبير الجيني SOD في فئران الوستر المعرضة للسجائر العادية والإلكترونية
قطاع الدراسات الصيدلية / علم الأدوية والسموم
تخصص البحث : علم الأدوية والسموم
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 12
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ حاكم بحلوك جبر - مؤلف رئيسي
علم الأدوية والسموم
Background: With the rapid increase in the use of electronic cigarettes as a prevalent form of nicotine, numerous studies have reported that their use has potentially negative health effects. Objectives: Evaluate and compare the effects of electronic cigarettes and traditional cigarettes on lung tissue. Study Design and Methodology: Twenty-five male Wistar rats were split into three groups: the EC group was treated to e-cigarette liquid vapor, the CC group to conventional smoke, and the control group without nicotine. Result: Significant alterations were observed in both experimental groups., including an increase in thickness of the alveolar wall, infiltration of inflammatory cells, collapsed vacuole in the wall of alveoli, and focal infiltration of lymphocytes in the wall of the bronchiole. Additionally, there was up-regulation of SODs gene expression in both groups. Conclusion: Histo-pathological analysis suggests that smoking and vaping are equally harmful to lung tissue. Real-time PCR analysis revealed significant up-regulation of SODs gene expression in conventional cigarettes and electronic cigarettes compared to the control group Keywords: E-cigarette, C-cigarette, lung, Histopathology, SODs genes
LAVENDER ESSENTIAL OIL IMPROVED DEPRESSIVE BEHAVIOR IN MICE AFTER INTERFERON-? ADMINISTRATION BUT NOT AFTER CYCLOSPORINE A ADMINISTRATION / زيت اللافندر العطرى يحسن السلوك الاكتئابي لدى الفئران بعد تناول الإنترفيرون ألفا ولكن ليس بعد تناول السيكلوسبورين أ
قطاع الدراسات الصيدلية / علم الأدوية والسموم
تخصص البحث : علم الأدوية والسموم
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 12
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ أزاده مسريبور - مؤلف رئيسي
علم الأدوية والسموم
The purpose of this study was to assess whether Lavender essential oil (Lav) could alleviate depressive symptoms induced by Interferon-? (IFN-?) therapy, which reduces tryptophan levels, or Cyclosporine A (Cyc) treatment that leads to psychological issues by inhibiting the mammalian target of rapamycin (mTOR) receptor. The study focused on mice as the subjects for evaluation. Lav 50,100,200 mcL/kg, and Cyc 40 mg/kg (all IP) and IFN-? 1600000 IU/kg SC were administered. The tests were performed after 6 days of consecutive injections on day 7. First total activity was evaluated by the locomotor test, following the splash test and then immobility time during the forced swimming test (FST) was measured. Sucrose preference was measured in order to test anhedonia. The selected treatments did not cause noticeable changes on the locomotor activity. IFN-? increased the immobility time during FST (178.6±10.4 s vs. control 125.8±17.7 s; P < 0.05) indicating depressive-like effect, and IFN-?-lav200 decreased it to (83.3±22.2 s, vs. IFN-?; P<0.01). The grooming time during splash test increased from 36.8±6.1s in IFN-? group to 106.3±9.6 in IFN-?-lav200 group (P<0.05). Sucrose preference increased to 82% following IFN-?-Lav200 administration. However, Lav did not cause a significant change on Cyc-induced depression. Conclusion: While Lav demonstrated a beneficial effect in mitigating IFN-?-induced depression in mice, it was not able to fully reverse Cyc-induced depression. This suggests that Lav exerts its antidepressant effects through distinct mechanisms that may not involve modifications to the mTOR pathways. Keywords: Lavender oil, Interferon-alpha, cyclosporine A, depression, animal experiment
POTENTIAL PROTECTIVE EFFECTS OF MESNA AND VITAMIN D SEPARATELY OR CONCOMITANTLY ON CYCLOPHOSPHAMIDE INDUCED HEMORRHAGIC CYSTITIS IN RATS / التأثير الواقي المحتمل لمادة الميسنا وفيتامين د كلا على حدة أو معا على التهاب المثانة النزفي المستحث بالسيكلوفوسفاميد في الجرذان البيضاء
قطاع الدراسات الصيدلية / علم الأدوية والسموم
تخصص البحث : علم الأدوية والسموم
Bulletin of Pharmaceutical sciences / / vol.46 الجزء الثانى لعام 2023 - 30/12/2023
تاريخ تقديم البحث 08/02/2024
تاريخ قبول البحث 27/02/2024
عدد صفحات البحث 22
سارة يوسف محمد( Sarahyousef30@yahoo.com - )
د/ منةالله عبدالله القاضي - مؤلف رئيسي
علم الأدوية والسموم
Background: Hemorrhagic cystitis (HC) is the most common adverse effect of cyclophosphamide (CP). We assessed the protective role of Mesna and vitamin D against CP induced HC. Methods: This experiment was performed on 64 rats; CON group (control), CP group (sham cyclophosphamide), M group (Mesna), CL group (low dose calcitriol), CH group (high dose calcitriol), VD group (cholecalciferol), CM group (calcitriol & Mesna), and VDM group (cholecalciferol & Mesna). Results: Mesna and vitamin D revealed a significant decrease in visceral pain score, bladder index, malondialdehyde (MDA) and tumor necrosis factor alpha (TNF-?) levels, caspase expression, and a significant increase in total antioxidant capacity (TAC) versus the CP group. Also, the VD, CH, CM & VDM groups revealed a significant decrease in TNF-? level and caspase, microtubule-associated protein light chain 3-II (LC3-II), and sequestosome-1 (SQSTM1/p62) expression versus the M group. The CH group showed a significant increase in serum calcium versus other groups. Conclusion: Vitamin D increased the uroprotective effect of Mesna, which is attributed to its ability to restore autophagy flux. Keywords: Hemorrhagic cystitis, cyclophosphamide, Mesna, calcitriol, and cholecalciferol
جميع البيانات التي يتم تسجيلها على النظام هي ملك للجهة صاحبة هذه البيانات
لا يتم الاطلاع على هذه البيانات من قبل مهندسي المركز إلا للضرورة القصوى ولغرض الدعم الفني وبالاتفاق مع الجهة صاحبة البيانات شفوياً أو كتابياً
لا يتم إعطاء أي بيان من بيانات النظام لأي فرد او جهة فرعية تنتمي للجهة صاحبة البيانات او لأي جهة أخرى إلا بموافقة كتابية من الجهة صاحبة البيانات
يمكن استخدام البيانات المسجلة على النظام بهدف عمل إحصائيات يستفاد منها فى قياس اداء النظام وكفاءة استخدامه من قبل مستخدميه
يتم تأمين جميع البيانات الخاصة بالنظام من خلال تأمين الحاسب الخادم الذي يستضيف البيانات ومن خلال تأمين انتقال البيانات بين جهاز المستخدم والحاسب الخادم وكذلك تأمين واجهة النظام التي يستخدمها المستخدم
كل مستخدم للنظام مسئول مسئولية كاملة عن كلمة المرور الخاصة به وعليه اتخاذ كافة الاحتياطات الممكنة حتى لا يستطيع أى شخص آخر الحصول عليها
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