THE POTENTIAL EFFICACY OF ALOE VERA GEL AND YUCCA SCHIDIGERA EXTRACT ON GROWTH PERFORMANCE, INTESTINAL LESIONS AND INFLAMMATORY RESPONSE IN BROILER CHICKENS CHALLENGED WITH COCCIDIA / الفعاليه المحتمله لجل الصبار ومستخلص اليوكا على مقاييس النمو والافات المعويه والاستجابه الالتهابيه فى دجاج التسمين المعدى بالكوكسيديا
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal
تاريخ تقديم البحث 24/06/2021
تاريخ قبول البحث 06/07/2021
عدد صفحات البحث 16
اسماء رفعت احمد ابراهيم
غاده علام عبدالدايم( anasesra@gmail.com - ) - مؤلف رئيسي
This study was conducted to investigate the effect of Aloe vera gel and Yucca schidigera extract supplementation on growth performance, oocyst shedding, intestinal lesions and inflammatory response in broilers challenged with coccidia. Two hundred and ten, one-day old Cobb chicks were randomly allocated to seven equal groups (30 birds/group). (G1): non-infected non-treated group, (G2): infected non-treated group, (G3)&(G4): infected and treated with Aloe vera gel 5gm/L and Yucca schidigera extract 200 mg/ L respectively from 7th day of age till the end of the experiment, (G5)&(G6): infected and treated with Aloe vera gel 5gm/L and Yucca schidigera extract 200 mg/L respectively from 15th day of age till the end of the experiment and (G7): infected and treated with Amprolium 1g/ 2 L from 15th day for five successive days. Chickens in infected groups were challenged with 50,000 sporulated oocysts of field strain of Eimeria spp. on the 14th days of age. The average body weight gains, feed intake and feed conversion ratio were recorded all over the experimental period. The anticoccidial evaluation post infection depended on severity of bloody diarrhea, lesion scores as well as the oocyst shedding. In addition, histopathological changes in intestine and serum level of inflammatory cytokines were evaluated. Aloe vera gel and Yucca schidigera extract supplementation were able to mitigate the devastating effects of coccidia challenge in broilers. Growth performances represented by bodyweight gain, and feed conversion ratio were significantly improved. The Lesion score and oocysts shedding were significantly reduced as well as the serum level of pro-inflammatory (IL-6 and IL-1?) were significantly down regulated in challenged treated groups. The histopathological changes of intestine were ameliorated in treated supplemented groups. In conclusion, Aloe vera gel and Yucca schidigera extract can be a promising candidate to be used as a natural, low cost alternative to control coccidiosis in chickens.
EFFECT OF MIXTURE OF SODIUM CHLORIDE AND POTASSIUM LACTATE ON THE VIABILITY OF TOXOPLASMA GONDII IN MEAT / تأثير خليط من كلوريد الصوديم ولاكتات البوتاسيوم على حيويه المقوسة القندية فى اللحوم
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal
تاريخ تقديم البحث 24/06/2021
تاريخ قبول البحث 06/07/2021
عدد صفحات البحث 17
اسماء رفعت احمد ابراهيم
باسم رفعت نجيب( basemnageib@gmail.com - ) - مؤلف رئيسي
The present study is a trial to implement an accurate and easily detectable test applied on meat juice to detect T. gondii. Besides realization the ability of table salt mixture to deactivate T. gondii. In the present study seroprevalence of T. gondii in sheep and cattle meat juice by LAT was 21.43% and emphasis by PCR was 17.14% which indicate very strong agreement between LAT and PCR as a reference test (Kappa = 0.862) with high specificity (94.83%) of meat juice LAT in comparison with PCR and strong relationship between these two tests (Correlation coefficient r = 0.871, p<0.0001). Sheep meat showed a high prevalence of T. gondii by both LAT and PCR tests (42.86%, 34.29%), respectively. No infection was recorded among the examined cattle meat. There was a very high statistical significance between the prevalence of infection among sheep and cattle by both tests. Both ewes and rams showed the same prevalence of T. gondii 42.9% without showing statistical significance according to the sex of the meat animal. By mice bioassay for salt mixture on the viability of T. gondii, a mixture of (2% sodium chloride and 1.4% potassium lactate) was able to deactivate of T. gondii cyst in sheep meat after 8 hours from exposure, while low concentration (1% of both mixture components) has no effect.
HISTOPATHOLOGICAL AND BIOCHEMICAL CHANGES OF ACUTE KETOPROFEN INDUCED NEPHROPATHIC LESIONS IN RATS / التغيرات النسيجية المرضية والبيوكيميائية للآفات الكلوية الحادة الناجمة عن الكيتوبروفين في الجرذان
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal
تاريخ تقديم البحث 24/06/2021
تاريخ قبول البحث 07/07/2021
عدد صفحات البحث 20
اسماء رفعت احمد ابراهيم
اميره سيد صادق( amira.sayed4494@gmail.com - ) - مؤلف رئيسي
The current work was undertaken to evaluate the nephrotoxic effect of Ketoprofen on adult male rats. Eighteen rats were divided into two groups. Ketoprofen- received group (I) included 10 rats were administered Ketoprofen at a therapeutic dose of 13.5 mg/kg by I/M injection daily for 4 successive weeks. Five rats were randomly selected from group I and sacrificed at 2 and 4 weeks of the experiment. The control group (II) that received olive oil included 8 rats, where 4 rats were sacrificed after 2 weeks and the rest of rats were sacrificed after 4 weeks. Tissue specimens from kidneys of all groups were collected for histopathological examination as well as the serum was obtained for the determination of biochemical parameters. The histopathological examination of group I showed glomerular changes such as expanding of glomerular matrix, glomerular sclerosis and congestion of glomerular capillary in the cortex. Renal tubular degeneration and necrosis accompanied with infiltration of inflammatory cells in interstitial tissue in both cortex and medulla were also observed. The biochemical results revealed that animals in group I showed a significant increase in malondialdehyde, creatinine, and urea compared to the control group, while total antioxidant capacity was numerically decreased. In conclusion, the therapeutic dose of Ketoprofen caused damage in kidney tissue even if was taken for a short period as well as altered biochemical parameters.
VIRULENCE OF ISOLATED PSEUDOMONAS AERUGINOSA INFECTING DUCKLING AND ANTIBIOTIC RESISTANCE WITH AN EXPERIMENTAL TREATMENT TRIAL / الضراوة لبكتريا السودوموناس ايرجينوزا المعزولة من البط الصغير ومقاومته للمضادات الحيوية مع محاوله تجريبيه للعلاج
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal
تاريخ تقديم البحث 24/06/2021
تاريخ قبول البحث 06/07/2021
عدد صفحات البحث 17
اسماء رفعت احمد ابراهيم
مصطفى صفوت عبده( dr.mostafaabdou@yahoo.com - ) - مؤلف رئيسي
The present study was conducted in Kafrelsheikh governorate to isolate P.aeruginosa from 120 ducks, sampling from cloacal swab, internal organs and tracheal swab of freshly dead, healthy and diseased ducklings at different ages which showed depression, emaciation, ocular and or nasal discharges, diarrhea and sometimes enlargement of hock joint and had lesions of airsacculitis, congested liver, catarrhal enteritis and sometimes presence of gelatinous material in the hock joints with rate of isolation of 16.66% (20 samples were positive). Serological identification of suspected isolates of P. aeruginosa showed that 16 isolates of polyvalent I group I and 4 isolates of polyvalent II group J. Susceptibility of the isolated P. aeruginosa for 11 antibiotics demonstrated that the highest resistance was noted against Erythromycin, Oxytetracycline, Ampicillin and Amoxicillin (95%). The highest sensitivity of P. aeruginosa was observed against Florphenicol (80%) and it used for treatment in experimental design and give good results. Multiplex PCR used to identify virulence represented by toxA gene, lasI gene and oprL gene of P. aeruginosa show that all isolates where positive for the 3 examined virulence genes and these indicate that all isolates were highly virulent strains and antibiotic resistance genes represented by qnrS gene, blaCTX gene and mexR gene of P. aeruginosa showed that all isolates where positive for the 3 examined AB resistance exept isolate No 4 were negative for blaCTX and these indicate that all isolates were highly AB resistance exept isolate No 4 was not have blaCTX resistance gene. Experimental infection was done to study the Pathogenicity of P. aeruginosa which isolated from diseased ducklings on newly hatched ducklings with dose of infection of 0.2ml of 1x107 cfu/ml s/c and use the potent antibiotic florphenicol for treatment of experimentally infected ducks. Our study proved that virulence genes owned by the P. aeruginosa confirming its pathogenicity for ducks, especially in the presence of oprL gene which plays a great role in antimicrobial resistance, so biosafety was recommended for hatcheries and farms, the hygiene, cleaning and disinfection will reduce P. aeruginosa spreading in the farms.
EFFICIENCY OF FOUR DISINFECTANTS AGAINST EIMERIA TENELLA ISOLATES from EGYPTIAN CHICKENS (IN VITRO ASSESSMENT) / كفاءة أربع مطهرات ضد معزولات الايميريا تينيلا من الدجاج المصري (تقييم معملي)
Background: Control of coccidiosis depends, in addition to the commercial preparation of herbal extracts, on good sanitation and litter management, along with the use of medication or vaccination programmes. In addition to the chemotherapeutic treatment of coccidiosis early disinfection of the poultry houses should be done using various disinfectants for controlling presence of oocysts. The objective of this research was to evaluate in vitro the action of four different coccidicidal disinfectant including (Quaternary ammonium compounds QACs, chlorocresol, glutaraldehyde and kilcox) on oocysts of Eimeria tenella. Methods: In this study the action of four coccidicidal disinfectant including (Quaternary ammonium compounds QACs, chlorocresol, glutaraldehyde and kilcox) on both sporulated and unsporulated oocysts of Eimeria tenella was evaluated in vitro. E. tenella Oocysts were obtained from naturally infected Egyptian native breed chickens. The oocysts were exposed to the disinfectants at different concentrations and different contact times. The efficacy of the disinfection was assessed by either destruction of sporulated oocyst or inhibition of sporulation. Results: It was observed that the most efficacious disinfectants against unsporulated and sporulated E. tenella oocysts was kilcox followed by chlorocresol, while QACs and, glutaraldehyde were less effective. kilcox sporulation inhibition reached 100 % on unsporulated E. tenella oocysts,and their destructive effect reached 99% on sporulated oocysts. furthermore, the results showed that both inhibitory and destructive activity of the tested disinfectants was significantly increased by increasing their concentrations and/or the contact time.
EFFECT OF PROPOLIS ON THE IMMUNE RESPONSE AND MEAT QUALITY IN EXPERIMENTALLY ESCHERICHIA COLI INFECTED BROILERS / تأثير البروبوليس على الاستجابة المناعية و جودة اللحم فى بدارى التسمين المعداه اصطناعيا بالميكروب القولونى
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal
تاريخ تقديم البحث 24/06/2021
تاريخ قبول البحث 06/07/2021
عدد صفحات البحث 35
اسماء رفعت احمد ابراهيم
منى سعيد ابراهيم سليم( dr_mona.saidibrahim@yahoo.com - ) - مؤلف رئيسي
نجلاء عباس الطيب عبد الحميد
هالة محمد اسماعيل حبيب
Propolis – E. coli - immune response - meat quality
The present study was conducted to evaluate the effects of propolis on performance, immune response and meat quality of broiler chickens infected with E. coli. A total of 120 day old chicks were divided randomly into 4 equal experimental groups. The 1st group (G1) was fed a basal diet and infected with E. coli (1x108 CFU) at 5 days of age. The 2nd group (G2) was fed a basal diet supplemented with propolis (400mg/kg diet) and infected with E. coli (1x108 CFU) at 5 days of age. The 3rd group (G3) was fed a basal diet supplemented with propolis (400mg/kg diet). The 4th group (G4) was fed a basal diet and served as a control. Propolis supplemented groups showed a significantly increased body weight, decreased mortality, decreased reisolation frequency of E. coli from internal organs and early recovery of infection. Propolis improved significantly the phagocytic activity in both supplemented groups. Total leukocytic count was significantly increased in propolis supplemented group with significant increase in lymphocytes and concurrent decrease in heterophils. Propolis improved the Newcastle disease vaccine antibody production in both supplemented groups. Propolis treated groups have significantly higher breast muscles protein concentration and lower fat content. Also, it showed a significant reduction on the bacterial load in the examined samples in comparison to the samples of the non supplemented groups.
ISOLATION AND IDENTIFICATION OF ORNITHOBACTERIOSIS from BROILER CHICKENS IN NEW VALLEY AND ASSIUT GOVERNORATES /
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal
تاريخ تقديم البحث 24/06/2021
تاريخ قبول البحث 06/07/2021
عدد صفحات البحث 16
اسماء رفعت احمد ابراهيم
محمد شاكر عبدالحافظ( drmohammed.abdelhafez@gmail.com - ) - مؤلف رئيسي
محمد خميس محمد
سطوحى احمد سطوحى
مصطفى عبدالمطلب شحاتة
محمد عادل سليمان
ORT, broilers, PCR, challenge
Respiratory infections are the most dangerous diseases facing poultry industry. Ornithobacteriosis is a respiratory disease in chicken broiler farms, it causes severe economic losses (high condemnation rate at slaughter, high treatment costs). Scientific research papers should be focused on the causative agent in order to make a plan for the ideal steps for its isolation and identification, in addition to prevention and control of this infection. The present work was conducted to isolate and identify Ornithobacterium rhinotracheale (ORT) in chicken broilers. A total of 195 samples (trachea, lungs and air sacs) were collected from dead and sacrificed broiler chickens aged (20 - 42 days old) from different broiler chickens farms in Assiut and New Valley Governorates for possible recovery of ORT. Samples were cultured on blood agar plates under 7.5 – 10 % CO2 tension at 37?C for 48 hours. Bacteriological examination used for isolation and identification of the ORT bacteria (cellular and colonial morphology, biochemical reactions, API 20 NE and growth on MacConkey’s agar) showed 32 suspected isolates at percentage of 16.4%. Results of isolation by conventional method were confirmed by molecular identification using Polymerase Chain reaction technique (PCR); which revealed the positive detection of only 5 strains of ORT and produced the predicated 625 bp amplification product. Serotyping of the isolates by using agar gel precipitation test proved that all isolates belonged to serotype A. Challenge of broilers aged 14 days old with ORT strain alone or combined with live ND vaccine by spraying method caused mild respiratory manifestations with tracheitis, pneumonia and mild airsaculitis. While Infection of chicks with ORT via spray combined with prior administration of ND vaccine (stress factor) caused a moderate respiratory signs and more severe lesions. from the results obtained in this work, we can conclude that: The prevalence of ORT infection in broiler chickens in Assiut and New Valley governorates was 2.56% from living and scarified birds. The current investigation revealed that the isolates of ORT bacteria belonged to serotype A. Post mortem lesions especially yogurt like exudate in the abdominal air sac considered very pathognomonic in the tentative diagnosis of ORT infection. While PCR technique was proved to be more sensitive and specific for detection of ORT pathogen.
EFFECT OF VITAMIN A AND D3 SUPPLEMENTAION ON THE GROWTH PERFORMANCE OF NILE TILAPIA (OREOCHROMIS NILOTICUS) / تأثير فيتا مين أ ، د3 على الأداء الانتاجى للبلطى النيلى ( ايروكروموس نيلوتيكس)
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal
تاريخ تقديم البحث 24/06/2021
تاريخ قبول البحث 07/07/2021
عدد صفحات البحث 13
اسماء رفعت احمد ابراهيم
سمير يوسف حسين - مؤلف رئيسي
محمد متولى أحمد
أسماء أحمد محمود( asmaa_bakr57@yahoo.com - )
Growth, Nile tilapia, Vitamin A, Vitamin D3, feed supplements
This study was conducted to evaluate the effect of supplemental levels of vitamin A (0; 20,000 and 40,000 IU/kg/diet) and 10 mg vitamin D3/kg diet on the growth performance of Nile tilapia, (Oreochromis niloticus). The180 fish were randomly divided into four treated groups each of 45 fish and each group was divided into 3 subgroups (15 fish / unit). The fish groups fed twice daily at 3% of body weight for 10 weeks. The body weight and total length were recorded biweekly all over the experimental period. At the end of experiment ten fish were scarified to determine hepatosmatic index (HSI), spleen somatic index (SSI), and gonadosomatic index (GSI). The results at the end of the experiment indicated that there was insignificant (P> 0.05) increase in body weight, body weight gain and total length. The specific growth rate (SGR %) increased insignificantly (P> 0.05) in fish group fed 10 mg vitamin D3/kg as compared to the control ones. While, the condition factor (K-value) decrease insignificantly (P> 0.05) in the fish group fed 20,000 IU vitamin A/kg than the control ones. Feed conversion ratio was insignificant (P > 0.05) decrease in treated fish with 10 mg vitamin D3 /kg as compared with other fish groups and control ones. Contrary, the hepatosomatic index (HSI) increase insignificantly (P > 0.05) as compared to the other fish groups and control. Moreover, all the doses of vitamin A and vitamin D3 had no effect on spleensomatic index (SSI) and gonadosomatic index (GSI). Results of the current study concluded that the studied levels of vitamin A and D3 had insignificant (P>0.05) effect on growth parameters of Nile tilapia, Oreochromis niloticus.
DIARRHEIC SYNDROME IN BROILER AND SOME WILD BIRDS CAUSED BY ESCHERICHIA COLI / متلازمة الإسهال في دجاج التسمين وبعض الطيور البرية التي يسببها الميكروب القولوني
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal
تاريخ تقديم البحث 06/07/2021
تاريخ قبول البحث 06/07/2021
عدد صفحات البحث 14
اسماء رفعت احمد ابراهيم
غادة عمر الدمرداش - مؤلف رئيسي
فاطمة عامر
هبة رشدي( roshdy2019@yahoo.com - )
broilers chicken, E. coli, resistant genes, and antimicrobial resistance, Wild birds.
E. coli is a Gram negative bacterium, although it is normal intestinal inhabitant but some strains due to their virulence genes play a major role in causing diarrhea in birds. In the present study, a total of 150 fecal swabs from (crows, egret wild birds and broiler chicken) collected from Giza, Fayoum city governorates, (50 each). The result showed, that isolation rate of E.coli was reported to be isolated in higher incidence in Fayoum governorate from crows, 48%, broiler chicken, 40% and egret wild birds 28% while Giza Governorate in where E.coli was isolated from crows, egret wild birds and broiler chicken ,an incidence of 20%, 12% and 20% respectively. The serogroups of E. coli strains that obtained by serological identification were from crows (O78, O91, O145, O127, O158, O119, O125 and O55), egret bird (O78, O158, O125, O119, O91 and O44) and chicken broiler (O78, O125 and O158). The results of sensitivity test for some E. coli isolates showed that they were highly resistant for to streptomycin (83.4%66.4%and 42.8%) in (crows, egret birds and broiler chicken) respectively. The results of multiplex PCR showed that phoA, virulence gene was detected in all E.coli serogroups while, Stx2, gene was detected in serogroups O78, O91 and O125 in crows only. (hly, eaeA and Stx1) virulence genes were not detected in all tested E. coli sergroups. On the other hand aadA1 gene was detected by some E. coli strains (7from crows and 2 from egret).
MITIGATION OF WELDING FUME WITH VITAMIN C / MITIGATION OF WELDING FUME WITH VITAMIN C
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal
تاريخ تقديم البحث 06/07/2021
تاريخ قبول البحث 06/07/2021
عدد صفحات البحث 6
اسماء رفعت احمد ابراهيم
B.S. OKEDIRAN - مؤلف رئيسي
S.A. AMID
K.Y. SULEIMAN
O.A. MAKINDE
B.A. ALABA
E. Olajide
fume, haematology, ascorbic acid,
Welding fumes are composed of fine and ultrafine particles, which when it gained access to the body system either via inhalation or as food or water contaminants can elicit various haematological and biochemical changes. In order to determine the haematological changes elicited by welding fumes and it’s mitigation by ascorbic acid, a total of fifteen male Wistar rats were randomly divided into three groups (A, B and C) of five animals per group. group A served as the control while group B were kept in enclosed welding environment for adequate inhalation of the welding fume. group C were equally kept in the enclosed welding environment but were daily orally administered 100mg of ascorbic acid for two weeks. At the end of two weeks of exposure, blood samples were obtained for haematological analyses. It was observed that exposure to the welding fumes predisposes the rats to macrocytic hypochromic anaemia and stress with evidence of neutrophilia and lymphopenia. These observed haematological perturbations were reversed by ascorbic acid back similar to that of the control group. It can be concluded that haematological perturbation induced by welding fume can be mitigated effectively by ascorbic acid without any deleterious effects.
Campylobacter jejuni (C.jejuni) is the leading bacterial cause of food born diarrheal illness and enterocolitis worldwide in human and young animals. A total of 140 samples (100 fecal samples of calves and 40 raw milk samples of cow) were collected from Dakahlia governorate dairy farms for isolation and identification of C.jejuni by using cultural, biochemical, molecular methods and detection of the virulent cadF gene using PCR. Moreover, the isolated C.jejuni subjected for antimicrobial susceptibility test. Then, we develop an experimental infection of rabbits by inoculation with 1 × 109 colony-forming units (cfu) of completely identified C.jejuni. Rabbits divided into 3 groups: infected untreated (G1), infected treated with enrofloxacine (G2), control (G3) to study pathogenesis, fecal inflammatory response, histopthology, immunohistochemistry and ultrastructural changes post inoculation in the three groups. Results revealed that, from 140 fecal and raw milk samples only 41 isolates were bacteriologically and biochemically identified as Campylobacter spp. Seven isolated strains were positively amplified for mapA gene specific to C.jejuni and carried the cadF virulence gene. C.jejuni isolates were resistant to amoxicillin and ampicillin and highly susceptible to norfloxacine and erythromycin. After oral infection with C. jejuni 90% of rabbits developed diarrhea with highly fecal inflammatory responses in G1, but mild in G2 (treated) and negative in G3 (control). Pronounced histopathologic changes were investigated in G1 during the acute phase (days 1 to 3) restricted on distal small intestine and colon including massive destruction of villi and loss of intestinal glands. The submucosa and muscularis mucosa showed the presence of edema with congested blood vessels, while hemorrhage was seen in the muscularis propria layer. The changes were mild and involved only the villi in treated group (G2), while abscent in control (G3). These results were confirmed by immunostaining, suggesting that C.jejuni is capable of invading deep intestinal tissues down to the submucosal layer in G1 while in G2 infection, the reaction was confined mainly to the villi, and was greatly reduced in the submucosa. Electron microscope showed all stages of invasion and associating damages from postinfection, colonizationa and villus damage. Thereby, the implementation of hyagenic practices during milking and proper handling of milk during calves feeding with regular monitoring of antibiogram profile are very crucial in preventing C.jejuni infection, colonization and intestinal damage and subsequently economic loss in dairy farm.
DIFFERENCES BETWEEN PHENOTYPIC AND GENOTYPE charACTERIZATION OF S. AUREUS ISOLATED from BOVINE MASTITIS IN EGYPT / الاختلاف بين التوصيف الظاهرى والجينى للمكور العنقودى الذهبى المعزول من الابقار المصابة بالتهاب الضرع فى مصر
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal
تاريخ تقديم البحث 09/08/2021
تاريخ قبول البحث 09/08/2021
عدد صفحات البحث 20
اسماء رفعت احمد ابراهيم
علاء الدين حسين مصطفى - مؤلف رئيسي
أحمد مصطفى حماد
مى عبد الله محمد عبد الله داود( mai_dawoud30@yahoo.com - )
bovine mastitis, coagulase positive staphylococcus (CoPS), coa gene, ica gene (biofilm formation gene), nuc gene, S. aureus
S. aureus is one of the most important causes of nosocomial infections, main contagious pathogens that can play a vital and important role in bovine mastitis in veterinary medicine causing high worldwide economic losses, where the primary reservoir harboring the pathogens is cow, and recently recorded as zoonotic microbe which able to transmitted from human to animal and vice versa, identification of staphylococcus species mainly S. aureus become more quickly and accurate with molecular technique as phenotypic technique due to multidrug resistance developed some mutation and production of biofilm which interference phenotypic identification, as nuc gene, and coa gene become the golden standard technique for identification S. aureus and the study concerned the differentiation between phenotypic and genotypic characterization of isolates collected from milk samples of bovine mastitis, as directed 157 phenotypic staphylococcus isolates on MSA media to biochemical phenotypic tests as 81/157 (51.6%) isolates of them were phenotypic S. aureus, and by molecular technique, 16S universal primer detected 141/157 (89.8%) were confirmed staphylococcus about 130/141(92.2%) were CoPS by coa gene detection, and 111/141 (78.7%) were typical S. aureus detected by nuc gene, as most of them produced biofilm that detected by ica gene in 81/141(57.4%) was the main cause of interrupting the phenotypic characterization by biochemical tests.
ELECTROLYTES CHANGES IN MALE RATS DEPRIVED OF FEED AND WATER /
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal
تاريخ تقديم البحث 09/08/2021
تاريخ قبول البحث 09/08/2021
عدد صفحات البحث 7
اسماء رفعت احمد ابراهيم
B.S. OKEDIRAN( okediranbabatunde@gmail.com - ) - مؤلف رئيسي
S.A. AMID
K.Y. SULEIMAN
A.S. ADAH
F. SANUSI
F.H. OLAIFA
Electrolytes, erythrocytes, plasma, rats
Electrolytes are of great importance in cell metabolism because they are important cofactors or coenzymes needed for normal functioning of cells. The disturbances of these electrolytes result in changes of cell pH and acid base balance. In order to determine the electrolytes changes following feed and water deprivation in male rats, a total of ten (n=10) male albino rats weighing (152 ± 3.50)g housed in metallic cage were used for this study. The rats were deprived of feed and water consecutively for six days. On the third and sixth days blood samples were obtained for electrolytes studies in the plasma and in the erythrocytes. There were significant increases (P<0.05) in plasma sodium, potassium, calcium, magnesium, chloride and bicarbonate concentrations in comparison to the basal parameters while significant decreases (P<0.05) in erythrocytes sodium, potassium, calcium, magnesium, chloride and bicarbonate concentrations in comparison to the basal parameters were observed. It can be concluded that deprivation of male albino rats of food and water for six consecutive days resulted in perturbation, stress, dehydration and redistribution of electrolytes in various compartments of the cell.
جميع البيانات التي يتم تسجيلها على النظام هي ملك للجهة صاحبة هذه البيانات
لا يتم الاطلاع على هذه البيانات من قبل مهندسي المركز إلا للضرورة القصوى ولغرض الدعم الفني وبالاتفاق مع الجهة صاحبة البيانات شفوياً أو كتابياً
لا يتم إعطاء أي بيان من بيانات النظام لأي فرد او جهة فرعية تنتمي للجهة صاحبة البيانات او لأي جهة أخرى إلا بموافقة كتابية من الجهة صاحبة البيانات
يمكن استخدام البيانات المسجلة على النظام بهدف عمل إحصائيات يستفاد منها فى قياس اداء النظام وكفاءة استخدامه من قبل مستخدميه
يتم تأمين جميع البيانات الخاصة بالنظام من خلال تأمين الحاسب الخادم الذي يستضيف البيانات ومن خلال تأمين انتقال البيانات بين جهاز المستخدم والحاسب الخادم وكذلك تأمين واجهة النظام التي يستخدمها المستخدم
كل مستخدم للنظام مسئول مسئولية كاملة عن كلمة المرور الخاصة به وعليه اتخاذ كافة الاحتياطات الممكنة حتى لا يستطيع أى شخص آخر الحصول عليها
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