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[9000892.] رقم البحث : 9000892 - |
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Genetic Diversity in Wheat Genotypes Using Random Amplified Polymorphic DNA (RAPD) / |
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قطاع الدراسات الزراعية / General |
| تخصص البحث : General |
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Assiut Journal of Agricultural Sciences / |
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يوسف خيرى انور ( 01226313930 ) |
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wheat, RAPD, genetic diversity, PCR, varietal identification |
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Genetic diversity among 75 wheat genotypes (66 hexaploids and 9
tetraploids) was studied using 6 RAPD primers. A total of 76 DNA bands were
generated by the 6 RAPD primers from all genotypes, of which 53 (70.23%)
were polymorphic. The total number of amplification products per primer ranged
from 6 (OPO06) to 16 (UBC03 and UBC09) with an average of 12.67 bands per
primer. The size of PCR products ranged from 1478bp (OPA01) to 91bp
(UBC03). A significant correlation (0.892, p<0.01) was observed between the
total number of bands and the number of polymorphic bands. The six primers
amplified 11 unique bands for specific genotypes that would prove to be highly
useful in genotype identification and designing the future breeding strategy. The
similarity coefficient values ranged from 0.68 to 1.00, indicating moderate to
high genetic similarityin tested loci among the wheat genotypes. The 75 wheat
genotypes were grouped into two major clusters within the dendrogram. Cluster I
had the tetraploid genotypes and the hexaploid genotypes were placed in cluster
II |
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