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[9000701.] رقم البحث : 9000701 - |
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Physiological And Pathological Studies On Macrophomina Phaseolina The Causal Organism Of charcoal Root Rot Disease In Bean / |
| تخصص البحث : General |
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Agricultural Research Journal Suez Canal University / / Volume 2 - 2003 |
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I. N. M. Ali |
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M. I. Ghonim |
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Heba M. Abdel-Nabi |
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M. I. Ahmed |
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beans - phaseolus vulgaris - pectin methyl esterase - PME - polygalacturenase - PG - cellulase |
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Pathogenicity tests proved that, all the tested isolates were pathogenic to Xera bean cultivar. The isolate, which isolated from sweet melon roots, was the most pathogenic one, followed by the isolate obtained from bean roots and the isolate obtained from sesame stem. The temperature requirement studies of the fungus in vitro revealed that the most favorable temperatures for linear growth of the tested isolates were 25C0-35°C0. On the other hand, at 15C0, linear growth was low and differed with the different isolates. Three infested barley seeds /bean seed was enough inoculums to cause disease incidence. Sesame was highly susceptible host followed by Wheat and Maize, which were moderate, however Cantaloupe was less susceptible host. Fungal culture filtrate at different concentrations reduced percentage of seed germination and had harmful effect on rootlet length and rootlet dry weight. The fungus exhibited activity enzyme (pectin methyl esterase, polygalacturonase and cellulase) in culture filtrate and/or extract of infected bean plants. |
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