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[9000690.] رقم البحث : 9000690 - |
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Response Of Chitinase And Phenoloxidase To Insect Growth Regulators And / |
| تخصص البحث : General |
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Agricultural Research Journal Suez Canal University / |
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M.M. Abdel Hafez |
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A.M. Aboul-Enein |
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M.A., Afifi |
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M.H. Al-Elimi |
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A.M. Farag |
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Chitinase, phenoloxidase, IGRs, biopesticides, Spodoptera littoralis |
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The present study was carried to evaluate the effects of the lethal dose (LC50) of abamectin and two
IGRs on cotton leafworm Spodoptera littoralis (Boisd.) from the point of biochemical aspects. Also, is
essential to know more about the mode of action of abamectin and to which related to, the chitin synthesis inhibitors or to the juvenoid compounds. The relative susceptibility of two strains (laboratory and
field strains) of the strains to three insecticides: diflubenzuron, pyriproxyfen and abamectin, was tested
using dipping technique. Values of the LC50 were calculated for each compound. The tolerance ratio was
determined on basis of the LC50 values of the two tested strains. Obtained results revealed that the field
strain exhibited different degrees of tolerance towards the three tested insecticides which can be arranged as follows: pyriproxyfen (10.5 folds), diflubenzuron (5.5 folds) and abamectin (5.3 folds) as compared to the laboratory strain. Chitinase activity decreased markedly after treating the insects with the
LC50 of the three tested insecticides when compared to untreated insect. The reduction in the activity
continued in case of diflubenzuron and abamectin. At the end of experiment the three tested compounds
caused great elevations in chitinase activity. The three tested compounds gave the same trend of reducing phenoloxidase activity during the tested time intervals when compared to control |
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