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[9000477.] رقم البحث : 9000477 - |
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Cryosurvivng and Fertilizing Abilities of Rahmany Ram Spermatozoa after Freezing-Thawing Using Untraditional Packaging Methods / |
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Agricultural Research Journal Suez Canal University / / Volume 10, No. 1 - 2010 |
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M.M. Awada |
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E.I Khalifab |
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Ram, spermatozoa, cryopreservation, pellets, straws |
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The most successful packaging techniques for frozen ram spermatozoa are pellets using dry ice and straws but there are economical and commercial pressures to use other methods of packaging techniques in developing countries. Semen was collected of the experiment using an artificial vagina. Ejaculates, from three different rams, containing greater than 70% progressive motility were pooled together and considered to be one sperm sample. Forty eight ewes were used in this study to test the fertilizing ability of ram spermatozoa. The thawed semen was assessed for total motility (TM), and morphology of spermatozoa characteristics (MSC). TM and MSC were assessed after thawing and 0, 2, 4 and 6 h of incubation at 37°C. The morphology of spermatozoa (dead sperm, abnormal tails and damaged head) was assessed by staining frozen-thawed spermatozoa with eosin-nigrosin dye. Fourteen sperm samples were extended, frozen, thawed and analyzed. Semen was diluted with Tris yolk fructose (TYF) diluent at rate of 1:8. Packaging techniques for frozen ram spermatozoa were compared to straws. Four packaging methods were compared to cryopreserve ram spermatozoa: straws, aluminum sacks, cachet strip tablets pellet and paraffin wax pellet. Ram spermatozoa frozen in straws and aluminum sacks exhibited higher post-thaw motility than spermatozoa cryopreserved in pellet form using either cachet strip tablets or paraffin wax. After incubation at 37oC, overall means showed that there was a progressive decline in the percentage motility over time. The post-thawing incubation had a significant (P < 0.05) negative effect on sperm motility as well as the percentages of morphological characteristics. After 6 h of incubation, straws and aluminum sacks methods maintained a better post-thaw motility and morphological characteristics than other methods of cryopreservation. The conception rates obtained were 59.17, 58.34, 50.00 and 42.27 from the four cryopreserving packaging techniques straws, sacks, tablets pellet and paraffin wax pellet respectively. In conclusion, ram spermatozoa survive cryopreservation more effectively in straws, or aluminum sacks. This technique of aluminum sacks packaging is simple, requires little equipment, is less expensive than using straws and may prove useful for cryopreserving ram spermatozoa in developing countries. |
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