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تصفح المحتوي RDA
التصفح حسب الموضوعات
التصفح حسب اللغة
التصفح حسب الناشر
التصفح حسب تاريخ النشر
التصفح حسب مكان النشر
التصفح حسب المؤلفين
تصفح الهيئات
التصفح المؤتمرات
التصفح حسب نوع المادة
التصفح حسب العلاقة بالعمل
تم العثور علي : 26
 تم العثور علي : 26
  
 
إعادة البحث

Thesis 2022.

Thesis 2021.

Book 2014
ISBN: 9783527332359

Book 2010
ISBN: 9780470229651 ,0470229659

Thesis 2017.
pharmacy - Abstract:
Historically
- plants have been used since ancient times and in folklore for the treatment of many diseases and illnesses including chronic diseases and cancer care. In recent years - plants have been getting more attention as drugs worldwide.
Achillea fragrantissima (Forssk.) Sch. Bip. is a desert plant which belongs to Asteraceae family and is known as Qaysūm in Arabic. We selected it as a member of family Asteraceae to be the plant under investigation. It grows in Sinai
- Egypt and is used in folk medicine by Bedouins for treatment of many diseases. In addition it has been tested for its anticancer activity. The broad aim of this thesis was to conduct a phytochemical investigation and biological confirmation of the plant Achillea fragrantissima (Forssk.) Sch. Bip. and its constituents as an anticancer agents illustrating their mechanism of action.
The initial part of this work focused on large scale extraction of the plant with different solvents
- bioactivity-guided fractionation led to the isolation of seven compounds followed by determination of the chemical nature of them. The isolated compounds are:
The known compounds Eupatilin 7- methyl ether (3)
- chrysosplenol D (4) - Cirsiliol (5) - β-Sitosterol-3-O-β-D-glucoside (6) and Cirsimaritin (7) while Piceol (1) and Veratric acid (2) are reported here for the first time from Achillea fragrantissima (Forssk.) Sch. Bip.
The second part of this work focused on evaluation of the in-vitro cytotoxic activity of the methanolic extract of A. fragrantissima (Forssk.) Sch. Bip
- its fractions and the isolated compounds 1 - 2 - 3 - 4 - 5 and 7 against different cell lines using the Sulpho-Rhodamine-B (SRB) assay. The extract - fractions (4 - 5) and the compounds 3 - 4 - 5 and 7 showed high cytotoxic activities when compared to appropriate reference drug (doxorubicin) and IC50 values (the concentrations of drug required to produce 50% inhibition of cell growth) were calculated for the isolated compounds.
The third part of this work focused on evaluation of the in-vivo antitumor activity of the methanolic extract of A. fragrantissima (Forssk.) Sch. Bip. and its isolated compounds 3
- 4 - 5 and 7 using Ehrlich’s Ascites Carcinoma (EAC) model in mice. A. fragrantissima (Forssk.) Sch. Bip. extract and its isolated compounds reduced the tumor weight compared to EAC-Control group. In addition - the compounds 3 - 7 treatments produced a dose dependent reduction in tumor weight. Determination of serum tumor necrosis factor – alpha (TNF-α) level highlighted that A. fragrantissima (Forssk.) Sch. Bip. extract and its isolated compounds significantly reduced its serum level compared to the EAC-Control group - where 3 - 5 - 7 groups produced a significant reduction in serum level of TNF-α compared to all other groups. Furthermore - A. fragrantissima (Forssk.) Sch. Bip. extract and the isolated compounds significantly increased the total antioxidant capacity (TAC) compared to EAC-Control especially compound 7. The histopathological examination revealed that A. fragrantissima (Forssk.) Sch. Bip. extract and its isolated compounds produced a better differentiation of tumor cells - with reduced nuclear pleomorphism and better formed tubular structures especially with high dose indicating the induction of apoptotic mechanism.
Finally
- an in silico study was performed as a step to elucidate the mechanism of the cytotoxic effect of the compounds isolated from A. fragrantissima (Forssk.) Sch. Bip - where the structure of cirsiliol (5) was docked into the active sites of the crystal structures of PI3K and Akt - two protein kinases that are significantly involved in prostate and breast cancer processes.
Key Words: Achillea fragrantissima (Forssk.) Sch. Bip
- anticancer - Ehrlich’s Ascites Carcinoma - Docking.
Aim of work:
Flora of Egypt especially in Sinai Peninsula is rich and diverse with medicinal and aromatic plants as well as herbs and spices. These plants have been used by local people in folk medicine for curing human and animal illnesses. The plants of genus Achillea as a part of Sinai Peninsula flora were and still a rich source of valuable natural products for treatment of many diseases; therefore
- this study was designed to fulfill:
1. Collection of the plant material from Sinai Peninsula in Egypt.
2. Bioactivity-guided fractionation to isolate active compounds using different chromatographic techniques.
3. Identification and structure elucidation of the isolated compounds
- using 1D NMR - 2D NMR and DEPT measurements.
4. In vitro cytotoxic and in vivo antitumor evaluations of the methanolic extract of Achillea fragrantissima (Forssk.) Sch. Bip. and its isolated compounds.
pharmacognosy

Book 2013. ,©2013
ISBN: 9781119967569 ,9781119967576

Thesis 2017
AD-MSCs: Adipose Derived Mesenchymal Stem Cells
ALCAM: Activated Leukocyte Cell Adhesion Molecule
ASCs: Adult Stem Cells
Bcl-2: B-cell lymphoma 2
bFGF: basic Fibroblast Grwoth Factor
BIR: Baculovirus IAP Repeat
BIRC1/NAIP: Baculoviral IAP Repeat Containing 1/NLR family Apoptosis Inhibitory Protein
BIRC2/cIAP1: Baculoviral IAP Repeat Containing 2/ cellular Inhibitor of Apoptosis Protein 1
BIRC3/cIAP2: Baculoviral IAP Repeat Containing 3/ cellular Inhibitor of Apoptosis Protein 2
BIRC4/XIAP: Baculoviral IAP Repeat Containing 4/X-linked Inhibitor of Apoptosis
BIRC5: Baculovirus IAP RepeatContaining 5
BIRC6:Baculovirus IAP RepeatContaining 6
BIRC7:Baculovirus IAP RepeatContaining 7
BIRC8: Baculovirus IAP RepeatContaining 8
BM: Bone Marrow
BM-MSCs: Bone Marrow Mesenchymal Stem Cells
BMP2: Bone Morphogenic Protein
CAFs: Carcinoma Associated Fibroblasts
CD 95: Cluster of Differentiation 95
CDKs: Cyclin-Dependent Kinases
cDNA: complementary Deoxyribonucleic Acid
CFU-Fs: Colony Forming Unit Fibroblasts
Ct: Cycle threshold
CTL: Cytotoxic T-Lymphocyte
dATP: deoxyadenosine Triphosphate
DCs: Dendritic Cells
dCTP: deoxycytidine Triphosphate
DDR:DNA Damage Response
DEPC: Diethyl pyrocarbonate
dGTP:deoxyguanosine Triphosphate
DMEM: Dulbecco’s Modified Eagle’s Medium
DNA: Deoxyribonucleic Acid
DNAase: Deoxyribonuclase
dNTPs: Deoxynucleotide Triphosphate
DPSCs: Dental Pulp Stem Cells
DR: Death Receptor
dTTP: deoxythymidine Triphosphate
E1: ubiquitin-activating enzyme
E2: ubiquitin-conjugating enzyme or UBC
E3: ubiquitin protein ligase
E4: ubiquitin chain-assembly factor
ECM: Extra-Cellular Matrix
EDTA: Ethylene Diamine Tetra Acetate
ELISA: Enzyme-Linked Immunosorbent Assay
ER: Endoplasmic Reticulum
ESCs: Embryonic Stem Cells
FasL: Fas Ligand
FBS: Fetal Bovine Serum
GAPDH: Glyceraldehyde 3-Phosphate Dehydrogenase
HeLa Cells: Cell line derived from cervical cancer cells taken “Henrietta Lacks”
HEp2: Human Epithelial type 2 cells
HGF: Hepatocyte Growth Factor
HKGs: Housekeeping Genes
HLA: Human Leucocyte Antigen
HLA-DR: Human Leukocyte Antigen - antigen D Related
HNSCC: Head and Neck Squamous Cell Carcinoma
HPRI: Human Placental Ribonuclease Inhibitor
HSCs: Hematopoietic Stem Cells
IAPs: Inhibitor of Apoptosis of Proteins
ICAM-1: Intra-Cellular Adhesion Molecule-1
IFN-γ/IFN-α/IFN-β: Interferon gamm/alpha/beta
IGF-1: Insulin-like Growth Factor-1
IL-6: Interleukin 6
Ink4: INhibitors of CDK4
iPS cells: induced Pluripotential Stem cells
IκB α: Inhibitor of kappa B alpha
JNK: c-Jun N-terminal Kinase
lacZ: bacterial beta-galactosidase
LIF: Leukemia Inhibitory Factor
MAPK: Mitogen Activated Protein Kinase
MCF-7: Michigan Cancer Foundation-7 (breast cancer cell line)
M-CSF: Macrophage Colony-Stimulating Factor
MHC:Major Histocompatibility Complex
ML-IAP: Melanoma Inhibitor of Apoptosis
MMLV: Moloney Murine Leukemia Virus
mRNA: Messenger Ribonucleic Acid
MSCs: Mesenchymal Stem Cells
MTT: Mosmann’s Tetrazole Test/ Methyl Thiazol Tetrazlium
NeuN: neuron specific protein
NF-κB: Nuclear Factor kappa B
NK: Natural Killer
NSE: Neuron Specific Enolase
OSCC: Oral Squamous Cell Carcinoma
PARP: Poly ADP-Ribose
PBS: Phosphate Buffer Saline
PGE2: Prostaglandin E2
PGF: Placental Growth Factor
pH: potential of Hydrogen
PI3K/AKT: Phosphoinositide 3-Kinase pathway/ AK murine Thymoma
PLA: Processed Lipoaspirate
PS: Phosphatidylserine
Rb protein: Retinoblastoma protein
RING: Really Interesting New Gene
Rpm: Rounds per minute
RQ: Relative Quantification
RT-PCR: Reverse Transcriptase Polymerase Chain Reaction
SCC: Squamous cell Carcinoma
SCF: Stem Cell Factor
SDF-1: Stromal Cell Derived Factor-1
SHEDS: Stem Cells from Human Exfoliated Deciduous
siRNA: small interfering Ribonucleic Acid
SMAC/Diablo: Second Mitochondria-Derived Activator of Caspase/Direct IAP binding protein with low pH
SPSS: Statistical Package for the Social Science
SVF: Stromal Vascular Fraction
TAFs: Tumor Associated Fibroblasts
TFG-β: Transforming Growth Factor beta
TNFR1: TNF Receptor type
TNF-α: Tumor Necrosis Factor- alpha
TRAF2: TNF Receptor Associated Factor 2
UBC: Ubiquitin-Conjugating enzyme
VEGF: Vascular Endothelial Growth Factor
VSEL: Very Small Embryonic- Like

Articles
Vol. 30, No. 2 (December 2003) /
   

Book 2013.
ISBN: 9780124071988 ,0124071988

Articles 2012.


من 3
 







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