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Abstract
A preliminary field-based investigative study was conducted for screening, possible detection and determination of presence of equine herpes virus-1, 4 and their antibody prevalence in blood sera collected from 230 adult and an aborted fetus of Native, Arabian and foreign horses in Qatar and Egypt. Using commercial kits, 99% (120 out of 121) of the screened serum samples belonging to the Qatari horses seroconverted to either EHV-1 or EHV-4 types. Of the total positive, 100% were anti-EHV-4 antibody positive versus 16% anti-EHV-1 antibody positive. All of the anti-EHV-1 antibody positive sera were also anti-EHV-4 antibody positive. Interesting enough, only 0.06% of the antibody positive serum samples were recorded originating from horses of a previous history of vaccination against the two viruses. None of the 49 corresponding peripheral mononuclear cells, randomly selected from 3 ELISA positive groups were real time PCR positive for either specific EHV-1 or 4 specific primer sets