الفهرس 
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Abstract
SUMMARY
The present study was conducted in the tissue culture
laboratory (Star moon company, Giza government) for Agriculture
during the period from 2013 to 2015.
The aim of this study was to evaluate the efficiency of different
soilless culture systems (Aeroponic, Deep water and Control) for prebasic
seed potato production, taking into account the origin of
propagative material seed (mini tubers and plants from in vitro
culture). This work was divided into two experiments.
1- Micropropagation of potato virus-free plantlet :
Potato virus-free plantlet (meristem – derived) 6-9 cm having
1-15 nodels grown in vitro (cvs, Spunta, Diamant and Hermes)
A- Rapid micropropagation of plantlets in vitro plantlets were
subcultural into shoots on inducing medium containing MS
salts supplemented with 3% sucrose, 1 mg/L IAA and 0.04
mg/L Kin solidified with 0.7% agar.
B- High quality planting material and rapid production of
many uniform plants have been obtained and the 0.3 mg/L
of both BA and Kin is the best concentration in the medium
since it gave the highest height, of plantlet, longest root,
high no. of branches or nodes and more weight of plantlet
than there concentration No. clear difference between the
cultivars was found (Spunta, Diamant and Hermes)
C- The influence of various sucrose concentrations (0, 30, 60
and 90 gm/L) on plantlet height., root length, number of
branches, number of nodes and plant weight, using three
cultivars Spunta, Diamant and Hermes) were studied the
data indicate that all growth plantlet height., root length,
19
SUMMARY
Mohamed M. Maamoun, (2017), Ph.D., Fac. Agric., Ain Shams Univ.
number of branches, number of nodes and plant weight)
increasing of sucrose concentrations over all cultivars used.
2. Soilless culture system
All shoots of the three potato cultivars which obtained from
the first experiment were transferred to the rooting medium
containing IBA at 1.0 mg/L. After two weeks the complete plantlets
produced were washed several times and transferred from the culture
tubes into plastic pots filled with peat-moss and perlite at ratio of 1:1
(v:v) and immediately transferred to green house under mist. The
plantlets were fertilized with El-Behairy, 1994 solution once a week
for a period of 40 days. After that the plants were cultured into
soilless culture using two systems i.e., aeroponic and deep water
system beside the control (sandy soil) Planting dates were 15th and
20th November in the first and second seasons respectively.
A – Plant growth
Data showed cleared that plant growth parameters were differed
among cultivars. Cv. Herms was significant superior cultivar for
obtaining the highest plant growth expressed as plant height, root
length, number of branches, leaf area, average fresh weight and total
chlorophyll in leaves compared with other cultivars i.e Spunta or
Diamant.
On the other side aeroponic system significantly is considered
more superior technique than deep water for producing the highest
results of the previous parameters.
B - Yield and its components
The mean tuber yields under soilless culture was higher when
the same material is left to produce tuber under conventional method
(soil culture).
19
SUMMARY
Mohamed M. Maamoun, (2017), Ph.D., Fac. Agric., Ain Shams Univ.
The aeroponic system was sufficient to encouraged potato
plants to give the maximum number and heaviest weight of mini
tuber than deep water system.
Herms cultivar of potato significantly gave the highest results
of number of tuber, at the same time gave the best tuber diameter and
weight as compare to Diamant and Spunta cultivars.
from all previous results we can conclude that tissue culture
is the best technique for producing plantlet free virus diseases. Also
we can conclude that Aeroponic system is considered the best system
for growing the potato plants which enhanced the seedlings to
produce the vigorous vegetative growth and at the same time were
sufficient to increase the quantity and quality of tubers