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Abstract The effects of the microbial flora associated with house fly Musca domestica and the effects of the two plant extracts of Limonium pruinosum and Calotropis procera on Musca domestica were studied. The results were summarized as follows. I- Bacteriological study: I.1- Microbial flora associated with the house fly Musca domestica: The microbial examination of the house fly Musca domestica revealed a heavy and very diverse microbial flora. The bacterial isolates from the house fly either externally or internally were found to belong to Escherchia coli, Salmonella sp., Shigella sp., Pseudomonas sp., Klebsiella sp., Proteus sp. and Bacillus sp. On the other hand Heamohilus bacteria were absent either externally or internally from house fly gut. While non-haemolytic staphylococci and streptococci represented 100% in both isolated sites. Also it was noticed that actinomycetes, yeasts and filamentous fungi were isolated only from external surface of the housefly, while they are completely disappeared in the housefly. I.2- Elimination of gut microbial flora of female, Musca domestica : The data showed that the norfloxacine antibiotic was the most effective agent against: Eschericia coli, Salmonella sp., Pseudomonas sp., Staphylococcus sp. and Streptococcus sp. Thus it is used in the present work to eliminate these bacteria al species from the gut of female Musca domestic flies. Other antimicrobial agents tested were less affected than norfloxacine on the previous bacterial species. I.3- Effect of the isolated midgut bacteria on different biological aspects of the housefly Musca domestia. The data showed that the mean number of eggs laid by the sterile females was less than that of the control ones. The absence of bacteria was highly decreased the hatchability percent, it significantly reduced to 59.6% in sterile flies. While it was 96.5% in the control group. The larval mortality was increased to 8.4% in the sterile female while it was 3.5% in the control ones. Also the pupal mortality was increased to 8% in the sterile females, while it was 1.8% in the control ones. |