الفهرس 
Metabolic rolesOnly 14 pages are availabe for public view
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Abstract
The endogenous sporulation rhythm in the fungus Leptosphaeria michotii is controlled at metabolic level by a non-circadian-oscillation, which is non-temperatufe-compensated and non-nutrient-compensated, and can be manipulated by metabolites and antimetabolites, in a specific manner, contrary to circadian systems.The asparagine-aspartate-oxalacetate -pyruvate pathway,whose enzymes expressed the same periodicity, plays an essential part in this simplified periodic model The asparaginase rhythmic activity is regulated by a reversible phosphorylation process. The second element of this pathway, aspartate aminotransferase, is regulated by a translation process. The third element of this pathway, alanine aminotransferase <GPT> was analyzed, and was found to be one form. It was purified to a state of apparent homogeneity complexed with a specific polypeptide of Mr 23,000; its physico-chemical characteristics, and the effect of diferent factors were determined. A polyclonal specific antibody was obtained from rabbit and used to amalyze the GPT activity rhythm using ELISA test. It was noticed that the cyclic variation of GPT activity rhythm was entirely correlated with the cyclic variation in the amount of GPT enzyme. The obtained data are consistent with the hypothesis that the alanine aminotransferase activity rhythm in L. michotii is caused by some mechanism controlling the efficiency of translation of OPT form. In addition, they corroborate that the synchronous working of the two different oscillating processes can determine the rhythmic pattern of a main metabolic pathway.