الفهرس 
Introduction
MaterialsOnly 14 pages are availabe for public view
 |  | from | 109 |  |  |
| | | from | 109 | | |
Abstract
In this study adapted isolates of Lactobacillus, as well as, Lactobacillus –Tetragenococcus transconjugants were used in bioconverting lactose whey via lactic acid to overcome the disposal problems of whey resulted from cheese industry and biosafety single cell protein with significant bioconversion efficiency than their parental strains. Conjugation was used in this study as a mechanism of DNA transfer to release a variety of Lactobacillus recombinants. Eight antibiotics were used for genetic marking Lactobacillus strains to be used in mating experiments as a selectable markers for isolating transconjugants. Five Lactobacillus strains were used in this study. Treatment of Tetragenococuus halophilus with different levels of temperature has been shown to result in the elimination of chloramphenicol resistance genes at 35Co, as well as, chloramphenicol, erythromycin, K2 Cr2 O7 and high salt tolerance at 40Co. five Lactobacillus strains in this study were used as recipients and only strain Tetragenococcus which was used as adoner.This study was an environmental friendly via recycling the whey resulted from cheese industry using different recombinants of Lactobacillus strains to reducing environmental pollution .