الفهرس | Only 14 pages are availabe for public view |
Abstract This study was conducted to evaluate the role of biological control against some soil-borne pathogenic fungi, R. solani, F. oxysporum f. sp. cucumerinum caused damping-off disease in cucumber through integrated plant diseases management. In- vitro experiments the antagonistic actions of some antagonistic fungi, T. harzianum, G. virens and G. roseum were examined against the previously isolated soil-borne pathogenic fungi. All antagonistic fungi reduced the radial growth of the tested pathogenic fungi on different types of media. T. harzianum, G. virens gave the best reduction of radial growth of pathogenic fungi. Aantifungal activity of some commercial essential oils (camphor, Thyme and Nigella) at different concentrations was examined on the previously tested soil-borne pathogenic fungi. Uusing commercial essential oils at all concentrations reduced the radial growth of all tested pathogenic fungi. Thyme and Camphor oils gave the best reduction of radial growth of pathogenic fungi. Topsin M-70 was the most effective as standard fungicide in-vitro in decreasing the linear growth of tested soil- borne fungi followed by Vitavax-300and Rhizolex-T. The antifungal activity of compost tea at different concentrations was examined on tested pathogenic fungi. Under green house conditions, antagonistic fungi, antagonistic bacteria (Bacillus subtilis and Pseudomonas flourescens) and plant essential oils individually and in combination with low doses of Topsin M-70 or compost tea as seed soaking reduced the percentage of pre and post-emergence damping-off disease of cucumber and increasing percentage of survived plants compared with control treatment. Two application forms of antagonistic agents, Pesta formulation and soil drenching were used to evaluate the antagonistic activity of bioagents against soil-borne fungi. T. harzianum, B. subtilis and camphor oil gave the best control against soil-borne pathogenic fungi. Using biocontrol agents, T. harzianum, B. sutilis and camphor essential oil as soil drenching individually and in combination with ½ recommended doses of Topsin M-70 as seed soaking was conducted under field conditions. All treatments highly decreased cucumber pre and post-emergence damping-off incidence and increased survived plants percentage. Also Vegetative growth and root growth characteristics of cucumber plants were increased when compared with the untreated control. Combination of bio-chemical treatments gave better disease control than bio control treatments individually. |