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Abstract
This work was carried out in the laboratory and greenhouse of Agric. Microbiology Dept. at the Faculty of Agriculture,
Zagazig University, Egypt, in order to isolate and select efficient
indigenous strains of Rhizobium leguminosarum bv. vicieae,
Azospirillium spp. and Azotobacter spp. which isolated from
different locations in Sharkia governorate to use them as bio-
fertilizers for broad bean (Vicia faba L.) and wheat (Triticum
aestivum L.) plants. Thirty two Rhizobium isolates were selected
as effective strains for nodulation on broad bean, 18 isolates of
Azospirillum, and 14 isolates of Azotobacter were efficient in
nitrogenase activity in liquid cultures. Five isolates showing high
values in nitrogen fixation of R. leguminosarum bv. vicieae and
the highest five effective isolates of each of Azotobacter spp. and
Azospirillum spp. were used for GA3, IAA and IBA production
in liquid cultures. The selected bio-fertilizers were examined on
the effect on growth and productivity of broad bean and wheat
plants which cultivated in both sandy and clay soils under
different levels of inorganic and organic N fertilizers in six
greenhouse experiments.