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العنوان
Histological study of quadriceps femoris muscle before and after mechanical injury in rabbits /
الناشر
Amani Ahmed Awad El-Hawwari,
المؤلف
El-Hawwari, Amani Ahmed Awad.
هيئة الاعداد
باحث / أمانى أحمد عوض الهوارى
مشرف / الحسينى إبراهيـم الدسوقى
مشرف / سـناء أحـمد الشـربينى
مشرف / سلوى على أبو العز جاويش
مشرف / سلوى على أبو العز جاويش
الموضوع
Bone Diseases, Metabolic-- Physiopathology.
تاريخ النشر
2001.
عدد الصفحات
122 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علم الأنسجة
تاريخ الإجازة
1/1/2001
مكان الإجازة
جامعة المنصورة - كلية الطب - قسم الانسجة والخلايا
الفهرس
Only 14 pages are availabe for public view

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from 138

Abstract

This work was carried out to study the effect of mechanical trauma on the histology and histochemistry ofthe quadriceps femoris muscle of the rabbit. This experimental model of skeletal muscle injury enabled us to study the degeneration and necrosis of skeletal muscle fibers in addition to the events of subsequent repair. Thirty male rabbits were used in this study. Their mean weight was 0.75 kgm. Each rabbit was subjected to mechanical injury by introduction of the needle of a syringe into the right quadriceps femoris muscle of the thigh and moving the needle inside the muscle several times. The left muscle was used as a control. The rabbits were sacrificed at one day, three days, one week, two weeks and three weeks after injury. Six animals were sacrificed each . time. Both quadriceps femoris muscles of each rabbit were excised and processed for histological and histochemical examination. For histological examination of the muscles, paraffin sections (longitudinal and cross) were prepared, stained with haematoxylin and eosin, mallory stain and phosphotungstic acid - haematoxylin. For histochemical examination of the muscles, fresh frozen sections were prepared and stained for the demonstration of succinic dehydrogenase enzyme activity. In sections stained for the demonstration of this enzyme, we measured the diameter of the different fiber types of the control muscles one, two and three weeks after injury at a . magnification of Xl 00 using the calibrated micrometer in the eye lens. We also measured the diameter of the regenerating muscle fibers at the previously mentioned durations .. All these data were statistically analyzed.