الفهرس 
Introduction
The liver cellsOnly 14 pages are availabe for public view
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Abstract
Hepatitis C virus (HCV) is considered to be the leading cause of liver disease in humans. p53 gene is a tumor suppressor gene that is located on the short arm of chromosome 17. p53 are involved in control of cell cycle, apoptosis and maintenance of genomic stability. The aim of the present study was to detect a tumor suppressor gene product in patients with viral hepatitis using immunochemical techniques. Liver tissues and serum samples were obtained from 116 HCV infected patients with different pathological classification of liver disorders. According to H&E stained liver tissues and pathological examination, patients were classified into 3 groups: Noncirrhotic group (50 patients ), Cirrhotic group (22 patients), Hepatocellular carcinoma group (44 patients). In addition, sera were collected from 20 healthy volunteers as a negative control group. Selected sera were resolved in SDSPAGE and the resolved polypeptides were stained with Coomassie blue. Western blot analysis of resolved sera revealed that the specific rabbit antip53 antibody identified a sharp band in patients sera at 53 kDa molecular weight. The serum p53 antigen was purified using gel electroelution technique an applied in ELISA as immunochemical probe. ELISA detected human p53 antibodies in 57 % of 116 of HCV patients; 54 % of noncirrhotic; 73 % of cirrhotic and in 52 % of HCC patients. The current study concluded that the antip53 antibodies can be detected early in HCVrelated different liver diseases. So, it can be used as a marker in order to assess the clinical implications and prognoses of HCVinfected patients related different liver diseases. The ELISA for antip53 antibodies is a convenient and specific test for the detection of humoral response to alterations in p53 gene expression.