الفهرس 
Introductionيوجد فقط 14 صفحة متاحة للعرض العام
 |  | from | 119 |  |  |
| | | from | 119 | | |
المستخلص
1. The success of regenerative root canal treatment depends on the preservation of stem cells from the dental pulp and urged to differentiate the bone, but the materials used to cleanse the canals have a harmful effect on these cells, so the search for alternative natural materials for them can have stimulating cell metabolism Purpose of the research:This study aims to evaluate the effect of rosemary acid on the differentiation of dental pulp stem cells into dental cell-like cells as a potential root canal drug in regenerative root canal treatment. Research Tools & Methods Dental pulp stem cells were isolated and distinguished using flow cytometry. The cells were treated with concentrations (0.25, 0.5, 1, 2.5, 5 mg/ml) of triple antibiotic paste and calcium hydroxide and concentrations (6.25, 12.5, 25, 50, 100 μm) of rosemary acid. Cell viability was assessed after 3 days, with further cell proliferation analysed over 3, 5 and 7 days using MTT testing The optical density was measured at 570 nm, which later made it possible to determine the correction and validity of the cell. The concentration and activity of the alkaline phosphatase enzyme of the cell were also determined. Von Koussa dye and red elizarin assay were used to evaluate late-stage calcification. Followed by a statistical significance test Results:After cell validity testing, 0.25 and 0.5 mg/ml of triple antibiotic paste and calcium hydroxide showed no significant difference for dental pulp stem cells compared to the control group. While doses of 1 mg/ml, 2.5 mg/ml and 5 mg/ml significantly reduced cell viability. However, concentrations of 6.25 μm and 12.5 μm of rosemary acid showed a significant increase in cell susceptibility to cells compared to untreated cells, rosemary acid showed a significant increase in cell susceptibility to growth when used at concentrations of 6.25 and 12.5 μm compared to triple antibiotic paste and calcium .Discussion:Rosemary acid has shown a significant increase in cell viability when used at concentrations of 6.25 and 12.5 μm compared to triple antibiotic paste and calcium hydroxide and significantly stimulates the differentiation of dental pulp stem cells Conclusions and recommendations Rosemary acid needs laboratory and clinical studies before being used as a substitute for the substances currently used in regenerative endodontics. Related topic headings (no more than10):Ca(OH)2,MTT,TAP,Viability,Rosemary,PCR.