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العنوان
A study on genetic polymorphisms of some immune genes in goat /
المؤلف
Ali, Osama Abd Ul-Monaim Muhammad Abd Ul-Azez.
هيئة الاعداد
باحث / اسامه عبدالمنعم محمد عبدالعزيز
مشرف / محمد محمد فوده
مشرف / أحمد ابراهيم عطيه
مناقش / بسمه محمد هندام
مناقش / هناء محمد السيد غانم
الموضوع
Gene polymorphism. Baladi goat.
تاريخ النشر
2024.
عدد الصفحات
online resource (86pages) :
اللغة
العربية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
21/7/2024
مكان الإجازة
جامعة المنصورة - كلية الطب البيطرى - قسم تنميه الثروه الحيوانيه
الفهرس
يوجد فقط 14 صفحة متاحة للعرض العام

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المستخلص

Dissertation Abstract Goats are extensively found in tropical places and are mostly raised for their meat, though they are also occasionally raised for their milk, hides, and fiber. Goat milk accounts for about 2% of global milk production, with an annual production of about 18.4 million tons. Goats have been an essential source of food, clothing, agricultural output, and environmental control for humans for many millennia. Small farmers choose them because they can adapt to nearly any climate and produce high-quality meat products from forage. This study was conducted for screening the polymorphisms in caprine LTF gene using PCR-DNA sequencing. And investigating the association between LTF gene polymorphisms and mastitis resistance/susceptibility in Baladi goats. Blood samples were obtained 30 Baladi does (15 healthy and 15 mastitic). DNA was extracted and PCR was conducted using primer for amplification of 430 bp of LTF gene from all females of the study. And all animals of the study produced the 430 bp of LTF gene. Then DNA sequencing was performed for 430 bp of LTF gene of healthy and mastitic does. DNA sequencing of healthy and mastitic does revealed a variation in nucleotide sequence in form of SNP. This identified SNP in the investigated LTF gene for Baladi goat was G299A and this SNP is non-synonymous that replaced cysteine amino acid into tyrosine amino acid. In thirty Baladi goat breeds (15 healthy and 15 mastitis), PCR-DNA sequencing of LTF gene revealed a nucleotide sequence variation in the form of SNPs between investigated healthy and mastitic does. By employing genetic markers along with normal goat selection, a promising opportunity exists to lessen the occurrence of mastitis thanks to these functional variations. The identified SNP may facilitate future methods for treating mastitis in goat.