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Abstract A total no of 39 Sea Turtles (30 Red eared turtle and 9 Green Turtle) collected from (Red sea , Pet stores and Giza zoo ). and 45 water samples collected from the same habitat of examined turtle . Isolates were identified as Aeromonas Hydrophila ,Vibrio alginolyticus. The Total prevalence of Aeromonas hydrophila among all examined Sea turtle species were (33.3%) while Total Prevalnce of Vibrio alginolyticus were(15.4%) . Out of 45 Water samples , 14 sample were identified as Aeromonas hydrophila with a percent of (31%) and 4 samples (8.8%) were identified as Vibrio alginolyticus. The highest percent of infection detected in isolates from Red sea. The total Buccal Positive Aeromonas hydrophila isolates from Green Turtle were 4(57%) and Red eared turtle were 10(52.6%) from total positive isolates while Cloacal isolates from Greenturtle were 3(42.9%)and Red eared turtle Cloacal positive isolates were 9 (47.4%). The total Buccal Positive vibrio alginolyticus isolates from Green Turtle were 1(33.3%) and Red eared turtle were 4(44.4%) from total positive isolates while Cloacal isolates from Greenturtle were 2(66.6%)and Red eared turtle Cloacal positive isolates were 5 (55.5%). Antimicrobial sensitivity tests revealed that isolates of Aeromonas hydrophila were highly sensitivity to Norfloxacin (12/14) (85.71%) , Gentamycin 10/14 (71.42%) and Ceftriaxone 9/14 (64.28%), While show highly resistant to Amoxicillin 9/14 (64.28%) , Ampicillin and Rifamycin (8/14) 57.14%. PCR assay of 20 isolates were genetically confirmed, 14 Aeromonas hydrophila isolates were positive to 16SrRNA gene at 625 bp and 5 positive isolates of vibrio alginolyticus genetically confirmed by collagenase gene. . asignificant five virulence gene have been detected in examined Aeromonas hydrophila isolates including, aerolysin was detected in 92.8% (13/14) of isolates with a specific band at 326bp, act ,alt was detected in 85.7%(12/14) with a specific band at 232,442 bp Respectively, lipase was detected in 78.5% (11/14) with a specific band at 382 bp While Ahcytoen was detected in 100% (14/14) with a specific band at 232 bp. This study showed that presence of antibiotic resistance genes in A. hydrophila isolates which encoded as (blaTEM, blaSHV, were detected in 42.86% (6/14) at 516 bp, 28.5% (4/14) at 392 bp., respectively. The sequence results of A. hydrophila isolate showed 99% identity with the reference strains reported on Gene Bank. The obtained nucleotide sequences were submitted in the Gene Bank with accession number (MW255045) and (MW254993) . By measuring the innate immunity in Sea turtles detected that turtle have a good immune system which can protect them from infection. |