الفهرس | Only 14 pages are availabe for public view |
Abstract This study was planned to detect the prevalence of Aeromonas hydrophila in some dairy farms where that 22 milk samples of cattle origin out of 380 examined cattle milk samples were positive for A. hydrophila with prevalence of 5.8% (n=22). The bacteriological examination of The environmental samples was carried out for detection of A. hydrophila , 10 milk tank samples, 10 water samples , 150 bedding samples and 10 animal feed samples and the results showed that, the prevalence of A. hydrophila in environment, the positive samples were 2 milk tank samples (20%), 2 water samples (20%), 11 isolates from bedding samples (7.3%) and no isolates from feed samples ( 0%). All isolates were confirmed as A. hydrophila by studying the morphological characters on the specific culture media as well as its characteristic biochemical reactions. The molecular study on the A. hydrophila isolates showed that 22 strains of A.hydrophilia, 14 strains (63.6%) carry no virulence genes and 8 strains (36.4%) carry aerA gene, 5 of them (22.7%) carry hlyA gene also, while 3 strains (13.6%) carry aerA gene only. Antibiotic sensitivity test showed that the results recorded the highest resistance of A. hydrophila to ampicillin and lincomycin (100%) followed by cefotaxime (90.9%). None of the tested 22 isolates was resistant to amikacin. The public health importance was studied especially with the results of the contaminated milk samples and the environmental examined samples. |