الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 181 |  |  |
| | | from | 181 | | |
Abstract
Breast cancer is the most common type of cancer in Egyptian females.
Polymorphisms in the angiogenesis pathway have been implicated previously in
cancer risk and prognosis. The aim of the current study was to determine whether
certain polymorphisms in the genes of vascular endothelial growth factor A
(VEGFA), vascular endothelial growth factor receptor 2 (VEGFR2), vascular
endothelial growth inhibitor (VEGI), and hypoxia-inducible factor-1α (HIF1A) are
associated with breast cancer development. The study included 154 breast cancer
patients and 132 apparently healthy age-matched females as a control group. VEGFA
rs25648 genotyping was performed using (ARMS) PCR technique; while VEGFR2
rs2071559, VEGI rs6478106, and HIF-1α rs11549465 were genotyped by the PCRRFLP
method. Serum levels of VEGF, VEGFR2, VEGI, and HIF1A proteins in breast
cancer patients and controls were measured by ELISA. There was a significant
association between the VEGFA rs25648 C allele and breast cancer risk (OR=2.5,
95% CI=1.7–3.6, p < 0.001). VEGFA rs25648 C/C genotype was significantly higher
in breast cancer patients vs. control (p < 0.001). Participants with the T/T and T/C
VEGFR2 rs2071559 genotypes had 5.46 and 5 higher odds, respectively, of having
breast cancer than those with the C/C genotype. For the VEGI rs6478106
polymorphism, there was a higher proportion of C allele in breast cancer patients vs.
control (P = 0.003). Moreover, the C/C genotype of VEGI rs6478106 was
significantly higher in breast cancer patients vs. control (P = 0.001). There was no
significant difference in genotypes and allele frequencies of HIF-1A rs11549465
polymorphism between breast cancer cases and control individuals (P >0.05). Serum
levels of VEGFA, VEGI, and HIF1A were considerably greater in women with breast
cancer than in the control (P < 0.001). In conclusion, the genetic variants VEGFA
rs25648, VEGFR2 rs2071559, and VEGI rs6478106 are associated with increased
breast cancer risk in Egyptian patients.
Keywords: VEGFA rs25648, VEGFR2 rs2071559, VEGI rs6478106, HIF1A
rs11549465, Breast cancer.