الفهرس | Only 14 pages are availabe for public view |
Abstract This work was conducted to identify and characterize RHDV in naturally and experimentally infected rabbits, tracing the histopathological changes in different organs and to evaluate the genetic diversity of RHDV circulate in different Egyptian provinces. Moreover, to determine the genetic relationship among such isolates at the molecular level during the period from January 2019 to May 2022.Ninteen suspected liver samples were collected from outbreaks that occurred in 9 provinces during 2019 and 2022. Ten liver samples out of 19 are positive with slide and plate HA test. HA titer ranged from 5 log 2 to 12 log 2. Positive liver homogenates are confirmed to RHDV with RT-PCR and histopathology. Further characterisation of selected 4 viral strains was performed by nucleotide sequencing of VP60 gene. Based on nucleotide sequence analysis, 3 isolates were identified as RHDV var2 strains, while one isolate was assigned as classical strain. |