الفهرس 
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Abstract
This study has focused on investigating a consortium of actinomycetes for biotreatment of lipid-rich wastewater. It has also, involved investing the reuse of the treated effluent in irrigation for studying the genotoxic effects on these crops. Olive mill wastewater, which was collected from the outlets of El-Fayrouze olive mill North Sinai Al-Arish was used as lipid-rich wastewater at the present study. The characterization of wastewater included physical, chemical, and microbiological analyses. The OMW was characterized by an intensive brown color to reddish-brown, which becomes darker during storage and strong specific olive oil smell, acidic and they showed a high rate of turbidity. Its phenolic content, organic content, and oil has exceeded the acceptable limits for discharging into our surfaces. A selection of five actinomycete strains (S10, S11, S35, S43 and O1) has illustrated different hydrolytic enzyme activities and physiological characteristics, were selected for further investigation in the present study. The phylogenetic analysis of the strains showed a similarity percentage (98%-100%) with their closest matches in the database. Two Streptomyces and one Kitasatospora strain were 100% similar to their closest match in the NCBI database. Strain S10 was 100% similar to strain Streptomyces carpaticus. The strain S11 showed 100% similarity to Streptomyces hydrogenans, while, the strains O1 displayed a
Summary
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100% similarity to Kitasatospora albolonga. Also, a remarkable high similarity percentage (>99.5%) was observed on S35 to Streptomyces hydrogenans. Strain S43 showed 98% similarity to the strain Streptomyces anulatus. The partial 16S rRNA gene sequence of the five strains were submitted in the GeneBank. The consortium, growing in olive oil mill wastewater in the batch experiment, showed 53 % oil reduction after 5 days; while individual strains showed fewer removal abilities. Efficient oil removal has been achieved by the attached actinomycete consortium growth in the microcosm (57% removal) after 10 days. This study demonstrated that actinomycetes can be used in the biotreatment of oil / lipid-rich wastewater to enhance treatment. Wastewater often contains genotoxic substances that can resist different stages of the treatment process. In the present study, randomly amplified polymorphic DNA technology was applied to evaluate the genotoxic effects of wastewater (treated and raw) irrigation Jatropha curcas. RAPD profiles obtained showed that both treated and raw wastewater were having genotoxic effects on Jatropha curcas. This was apparent by the appearance/disappearance of bands in the treatments compared with the control plants. from the 5 primers used, 123 bands were obtained. Irrigating plants with raw wastewater caused 15 new bands to appear and 18 to disappear. Treated wastewater caused only 22 new bands and the loss of 43 bands. This makes TWW less
Summary
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