الفهرس 
HYPERTENSIVE DISORDERS WITH PREGNANCYOnly 14 pages are availabe for public view
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Abstract
Osteoprotegerin (OPG), a key regulatory factor in bone metabolism, was documented also as a proangiogenic factor, which acts an important role in protecting vascular endothelial cells. Since preeclampsia has gradually been employed to be vascular diseases, we speculated that OPG might be associated with preeclampsia. So this study is to investigate the relationship between OPG and the pathogenesis of preeclampsia.
Objective: To correlate expression of osteoprotegerin in placenta and its association with preeclampsia. Also; if osteoprotegerin level in placenta differs with severity whether mild or severe case
Subjects and Methods: Our study is a prospective case-control study which conducted at tertiary care hospital at Ain Shams University hospital. It was performed on total of 90 patients whom 30 patients diagnosed as severe preeclampsia, 30 patients diagnosed as mild preeclampsia and 30 patients as control healthy pregnant to assess the change in osteoprotegerin level in placental tissue in preeclampsia patients by: ELISA: to detect OPG protein level. Real time PCR: to detect OPG mRNA.
Results: In this study, by comparing OPG levels (OPG protein by ELISA and OPG mRNA levels by PCR) expressed in placental tissue between preeclampsia patients and healthy control groups, it was found that there was high significant difference (p ≤ 0.01) between preeclampsia and normal patients. So the OPG levels in placenta can discriminate between preeclampsia and control healthy group. And results showed that changes in each group were very observable, Both OPG protein and mRNA levels were high significantly different in severe cases of preeclampsia than those mild cases.These indicated that the changes of OPG levels were more obvious in severe preeclampsia, suggesting that expression of OPG were consistent with the severity of preeclampsia.
Conclusion: OPG expression in placenta can discriminate between preeclampsia and control healthy group. Also , OPG levels were more obvious in severe preeclampsia, suggesting that expression of OPG were consistent with the severity of preeclampsia but were not correlated or predicting to complication related to preeclampsia or fetal weight.