الفهرس 
INTRODUCTION AND AIM OF THE WORKOnly 14 pages are availabe for public view
 |  | from | 126 |  |  |
| | | from | 126 | | |
Abstract
Background/Aim: Kinetin is an antioxidant, exerting a protective role for normal cells against toxic compounds. Cancer therapy can cause oxidative stress, inflammation and DNA damage, which attacks healthy cells and tissues, thereby leading to further damage and side effects.
Cisplatin the drug that is widely used to treat a variety of tumors has been postulated to induce cell toxicity through triggering the apoptosis of the cells. Recently, combination therapies of cisplatin with other natural compounds have been highly considered to reduce its toxicity.
Aim of the work: The aim of this study is to assess the protective role of kinetin in mammalian lymphocytes against Cisplatin toxicity in vivo.
Methods: 54 Wister albino rats weighing 150-185 gm separated randomly into 9 groups; the first group of control were given saline, groups 2 to 4, were given 0.25, 0.5 or 1 mg/kg kinetin for 10 days via intraperitoneal injection (IP) injection; group 5 was given a single IP injection of 7 mg/kg Cisplatin (Cis); and groups 6 to 9, were given, for 10 days, IP injection of 0.25, 0.5 or 1 mg/kg kinetin or 200 mg/kg vitamin C and Cis in the fourth day. During the experiment, samples of blood were taken by the perorbital method, or the orbital venous plexus bleeding, at three time points (day 1, 2, and 10). After blood was collected, lymphocytes were separated using Histopaque 1077.
Results : two issues were assessed by our results, the first is; the intrinsic activity of Kinetin in lymphocytes and the second is the protective properties of kinetin against cisplatin-induces lymphotoxicity. Injected rats by Kinetin alone enhanced level of antioxidants GSH , GPx and MDA and reduced the level of inflammation markers (CRP, IL-6, TNF alpha) in the cells , while rats given
cisplatin injection demonstrated a significant rise in oxidative stress, apoptosis and inflammatory markers in lymphocytes. The concurrent administration of kinetin and cisplatin significantly reduced the toxicity markers of cisplatin.
Conclusion: Kinetin has intrinsic activity and protective effect. Our results reported that, Kinetin protects rat lymphocytes from cisplatin cytotoxicity via reduced levels of inflammation and apoptosis also enhanced scavenging system.