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Abstract
Consumer awareness against synthetic preservatives as well as the high incidences of bacteria foodborne outbreaks worldwide and development of bacterial resistance to most of available antibiotics has directed the attention of food hygienists to search for new more effective natural antimicrobial substances as plant extracts against a wide range of bacteria e.g. L. monocytogen and salmonella spp. Therefore, the present study was planned to demonstrate the behavior of both L. monocytogenes and S. typhimurium toward effect of oil extract of some commonly used spices in beef burger during frozen storage at - 18{u00BA}C for three months. MIC of oil extract of ginger, clove, black pepper, mace, coriander, cardamom, capsicum, white pepper, and cumin against reference strains of S. typhimurium and L. monocytogenes. Was determined for both pathogens, after that the effect the most powerful spices against each pathogen was evaluated separately in beef burger during storage for three months at - 18{u00BA}C. Results of MIC showed that 4% (v/v) oil extract of ginger, black pepper, capsicum, white pepper, and cumin induced significant reduction in viable cell count of L. monocytogenes