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Abstract
A total of 88 Pasteurella multocida isolates were isolated from 256 samples (34.4%). Dead calves showed the highest percentage of P. multocida isolation (80%), followed by the emergency slaughtered calves, diseased calves then apparently healthy ones (42.9%, 32.7% and 20%, respectively). These isolates were confirmed as Pasteurella multocida microscopically by staining with Gram’s and Leishman’s stains, biochemically by traditional tests and by API 20E commercial kit then by PCR. A total of 221 serum samples were collected: 50 samples from apparently healthy animals, 156 samples from diseased animals and 15 samples from emergency slaughtered animals