الفهرس 
Title : Pharmacological study of possible effects of certain antihypertensive drugs in animals with complicated diabet
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Abstract
In this study, the effects of early renin-angiotensin system blockade using angiotensinconverting enzyme (ACE) inhibitor lisinopril and/or angiotensin receptor blocker valsartan on
renal nephrin and vascular endothelial growth factor (VEGF-A) gene expression were
investigated in diabetic-hypertensive rats. Diabetes and hypertension were induced in adult
Wistar rats using streptozotocin (STZ) (45 mg/kg, i.p.) and Nω-nitro-L-arginine methyl ester
(L-NAME) (60 mg/kg/12 h) for 4 consecutive days. Experimental animals were allocated into
six groups (n=6): normal control, diabetic control, diabetic-hypertensive control, and
(lisinopril-, valsartan- and combination-) treated diabetic-hypertensive groups (5
mg/kg/drug/day, p.o., for 21 days). Blood glucose, blood pressure, body weight, serum
albumin, creatinine, total protein and urea were measured and recorded every week. Nephrin
and VEGF-A gene expression was measured using real-time polymerase chain reaction. Renal
nephrin protein was measured using ELISA as well as nephrin immunostaining.