الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 132 |  |  |
| | | from | 132 | | |
Abstract
In the present study, a total of 120 Egyptian sole (Solea
aegyptiaca) with average body weights (50-70 g) and average
lengths (13-19 cm) were collected randomly and seasonally
from the Mediterranean-sea in Port Said Governorate, Egypt
from September-2019 to August-2020. Fish were found to be
infected with two different types of Gram-negative bacteria
(Vibrio alginolyticus and Pseudomonas stutzeri) with a total
prevalence of 50%. The highest prevalence of infection
recorded was due to V. alginolyticus (29.16%) followed by Ps.
stutzeri (20.84%). The highest total prevalence of bacterial
infection among the naturally infected fish was recorded in
summer season (33.34%), followed by spring (26.76%), then
winter (25%), while the minimal prevalence of infection was
recorded in autumn (15%). Molecular investigation of pure
isolates using specific primers were positive to 16S rRNA genes
of Vibrio and 16S rDNA genes of Pseudomonas spp. and gave
expected amplicons size of 663 bp and 618 bp length,
respectively. The histopathology of naturally infected Solea
aegyptiaca exhibited severe inflammatory reactions and
mononuclear infiltration with activation of melanomacrophage
centers as well as tissue necrosis.