الفهرس | Only 14 pages are availabe for public view |
Abstract Bee pollen extract (BPE) based polymer nanoparticles (BPENP) were created in this study by using bovine serum albumin (BSA) and being targeted with folic acid. Spectroscopic and microscopic methods were used to characterize the resultant nanoparticles. High flavonoid and polyphenolic contents were detected by HPLC. At 336 nm, it was found that BPENP provided the highest absorption by UV. The average particle size ranged between 25 and 40 nm. The inhibitory effects and IC50 values of the acquired BPE, BPENP, Avastin, or their combination at 24 and 48 hr were detected against A549 lung cancer cells and MCF-7 breast cancer. The combination therapy assay of BPENP and Avastin revealed a very significant synergism between the two drugs with a low combination index (CI). Mice groups are: group 1 received saline, whereas groups 2, 3, 4, 5, and 6 received a single dose of urethane, followed by weekly injections of butylated hydroxy-toluene (BHT). After the BHT injection, the mice in groups 3, 4, 5, and 6 received BPE, Avastin, BPENP, and BNENP + Avastin, respectively. The number and size of tumors decreased in group 6 compared to those in the other groups. The ratios of early and late apoptotic cells in Groups 3, 4, 5, and 6 (42.8%, 41.4%, 26.2%, and 45.4%, respectively) were lower than that in the untreated group by flow cytometry analysis of Annexin v. At the S phase of the cell cycle, the treatment groups (3, 4, 5, & 6) were significantly lower than group 2, whereas the combination group 6 were significantly higher than groups (2& 4). Treatment groups (3, 4, 5, & 6) levels in the mitotic phase were significantly lower than group 2 levels, whereas combination group 6 levels were significantly higher than individual groups (4& 5). |