الفهرس 
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Abstract
Platelet-Rich Plasma (PRP) is an autologous product isolated from whole blood by using gradient density centrifugation to concentrate a high concentration of platelets in a low concentration of plasma.
There is much evidence linking prp to successful postoperative wound healing, thus we attempt to use it to improve episiotomy wound healing.
An surgical treatment is a surgical incision made in the perineum to facilitate the delivery of a baby during a particularly challenging vaginal birth.
The use of platelet-rich plasma (PRP) aids in the prevention of episiotomy wound problems and accelerates the healing process.
Both hemostasis and wound healing play crucial roles in each surgical incision. Immediately after an injury, platelets clump together to create a plug that stops the bleeding. Platelet granules, which contain growth factors including PDGF, PGR, FGF, and interleukins, are released when the platelet membrane is depolarized by thrombin. These growth factors promote wound healing by promoting collagen synthesis, fibroblast proliferation, and early collagen maturation.
To this end, between May and December of 2021, the gynaecology and obstetrics departments at Minia university hospital and El.fashn general hospital prospectively studied 200 instances of spontaneous vaginal births requiring episiotomy. Following discussing the assessment process for the function of platelets rich serum in tissue regeneration in patients after episiotomy, all patients provided written informed permission.
Patients’ own blood was used to make autologous PRP in this research (autologous PRP). Ten millilitres (ml) of sterile venous After obtaining a blood sample from the antecubital vein, the blood was then placed in sterile tubes containing 3.2percentage - point sodium citrate as an anticoagulant.
The red blood cells (RBCs) and the white blood cells (WBCs) were separated by centrifuging the blood at 300xg for 5 minutes at 18°C (middle layer),
Without damaging the buffy coat, the top fraction (plasma & platelets) was transferred to a new sterile tube and centrifuged at 700xg for 17 minutes at 18°C to separate them. When plasma was centrifuged, the bottom layer, or platelet pellet, was around 3-4ml. Immediately after collecting the PRP, platelet activation was accomplished by administering 0.3 ml of 10% calcium chloride per 1 ml of PRP.
For the sake of sterility, all of the surgeries were carried out in a dedicated operating room.
The PRP was injected into the subcutaneous fat of the incision site using a cleaned syringe just before the skin was closed. Patients in group B (the control group) will have normal saline used to clean the subcutaneous tissue before skin closure.
Normal medical practise dictated that no drains be used, and all participants’ skin was closed with vicryl-1. Patients were checked on the first, seventh, twenty-first, and fourth days after surgery. To measure how well the wounds were healing, we used the Vancouver scar level (VSS), the edoema ecchymosed discharge approximation (REEDA) scale, and a visual analogue scale (VAS)
It is clear from this research that the PRP group had much faster wound healing than the control group.
Additionally, after day one postoperatively, the scar heals more quickly and has a more aesthetically pleasing look, and patients may tolerate more pain with less discomfort. When comparing the overtime scores of the two groups, the PRP group performed worse across the board.