الفهرس 
Title : Studies on Micrografting of Grape By Using Tissue
AbstractOnly 14 pages are availabe for public view
 |  | from | 176 |  |  |
| | | from | 176 | | |
Abstract
The success of varions in vitro micrografting methods of grape (Vitis vinifera L.) have been examined. The adult micronods of freedom were disinfeding and micropropagated to use as rootstock source. Also, carrent year micronods from mature trees of each variety sperior and flame were used as scions by using micronodes from Juveule branches. For shootlets proliferation MS medium supplemented with BAP (6-benzyl amino purine) at 0.2, 0.4, 0.6, and 0.8 ) mg\L and kin at (0.2, 0.4, 0.6, 0.8) mg\L.
In vitro callus production were induction and production by using various parts of explants and Ms Medium supplemeted with 2, 4 D at (2 mg/L. 2,4-D + 2 mg/L. NAA, MS with 2 mg/L. 2,4-D + 4 mg/L. NAA, MS with 4 mg/L. 2,4-D + 2mg/NAA and MS with 4 mg/L. 2,4-D + 4 mg/L. NAA ) to use callus in micrografting process.
The micrografting process by using freedom as rootstock and flame, superior as microscion was used six methods by using (sterile wire tie, sterile metal needle, solid sterile wax, aluminum foil, gel matrix and callogensis). The variables tested include grafting methods morphological characterization chemical profiles. The results indicate that the most successful media for shootlet proliferation was MS+0.6 BAP that gave 95% suruival for freedom, 0.4 PAP scored 92% suruival for flame and 90% for superior. Meanwhile, shoot No. 4.21, 5.21, 4.91 for the three explants cultured on leaves number 5.08, 4.67 and 4.54 leaves /shootlet. On the other hand kintien has positive effect on shootlet elongation at 0.8 mg /l, which scored 2, 2.02 and 1.99 cm for the three explants, respectively