الفهرس 
Title : A Study on the Effect of Ultraviolet-B Radiation on Strawberry Plants Molecular Defenses Against Botrytis cinerea Fungus
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Abstract
In this study, it was possible to inhibit B. cinerea fungal growth severity in
harvested rip strawberry fruits through the proactive priming of fruits resistance
by UV-B light (290-315 nm). We irradiated harvested fruits with different UV-B
light amounts (0, 0.33, 0.65, and 1.3 mJoule/cm2
) before artificially infecting them
with B. cinerea. The molecular confirmation using B. cinerea specific Cutinase A
gene estimated a reduction ratio of 50% in fungal DNA with fruits proactively
exposed to UV-B (1.3 mJoule/cm2
) compared to non-UV-B (0 mJoule/cm2
)
treated fruits which were totally rotted with velvety gray mold growth. To reveal
possible molecular mechanism behind UV-B induced resistance against B.
cinerea, the expression profiles of several resistance-relatedgenes were quantified
using real-time PCR assay in the fruits on day 1 and day 2after infection. UV-B
light significantly peaked up the expression of the pathogenesis-related (PR) gene
FaBG2-1, which encodes the fungal cell wall degrading enzyme β-1-3-glucanase.
Furthermore, the jasmonic acid (JA) biosynthesis key gene FaAOS was
dramatically induced after exposure to UV-Bwith infected or non-infected fruits
on day 2 in particular. Interestingly, volatile terpenoids linalool/nerolidol synthase
gene (FaNES1) expression was also elevated in response to UV-B treatment.
ABA signaling gene FaPYR1 was also found to be responsive after 2 days.
Collectively, it is suggested that UV-B proactive treatment could urge strawberry
ripe fruits biological defenses prior anypossible fungal infection. Thus UV-B
irradiation could be considered as a sustainable method for controlling fungal
infection in ripe strawberry fruits postharvest