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Abstract
A total of 167 samples were collected from the intestine, liver and fecal samples with count 75, 60 and 32 respectively from apparently healthy and dead rabbits suffered from diarrhea. These rabbits were bacteriologically examined for the presence of clostridium perfringens. The percentage of C. perfringens from diseased rabbits were 58.8%and the rate of isolation of C. perfringens from intestine, liver and fecal samples were 16%, 16.6% and 31.25%, respectively. Typing of toxinogenic isolates of C. perfringens from intestine (77.7 %type A and 33.3% type D), liver (67.5%) and fecal samples (30% type A). 12 C. perfringens type A isolates were tested against antimicrobial agent and were sensitive to doxycycline, tetracycline, amikacin, oxytetracyclin and erythromycine. And were resistance to cloxacilline, streptomycine and kanamycine. While 3 isolates type D were sensitive to pencillin G, cephalothin and amoxycilline and resistance to amikacin, cloxacilline. And kanamycin. 9 isolates of C. perfringens show MDR to 3 groups or more of antibiotics. PCR assay were applied on 15 isolates of C. perfringens on 16sr RNA gene which is species specific gene to clostridium spp. Which located in 722 bp. And all isolates show presence of this gene of search