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Abstract
This study was conducted to produce plants from inflorescences of date palm Sewi cv. through tissue culture technique. The present work study the effects of different auxins (2,4-D, NAA, NOA, IAA, IBA and picloram) at (10, 20 and 40mg/l) and types of sugar (sucrose, glucose and fructose) at 0.1 - 0.15 and 0.2M on direct somatic embryos and callus formation after eight months. Also the effect of different cytokinin combination, sugar alcohol, different media formula (NN- MS- B5- WPM) and complex addenda (Malt extract, Yeast extract and Casein hydrolysate) at concentrations 100, 200 and 400 mg/l and type of auxins IAA, IBA and NAA at concentrations 0.0 - 0.5 - 0.1 and 0.2mg/l on growth and development of produced embryos and callus were discussed on the study. The results showed that the addition of 40mg/l picloram induced the highest percentage (39.93%) of direct somatic embryo formation while the addition of 10mg/l picloram induced the highest percentage (59.96%) of callus formation. Sucrose is the preferred carbohydrate for direct and indirect somatic embryo formation percentages. Complex addenda promote callus growth and embryo formation. Either IBA or IAA induced the highest significant number of roots/shoot