الفهرس 
Studies on hemoparasites infection in police dogs
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Abstract
Dogs serve as hosts for great numbers of parasites like Babesia and Hepatozoon.
This study aimed to identify the Babesia vogeli infecting police dogs of different
breeds, ages, and sex in police K9 dogs in Egypt using conventional PCR sequencing
and phylogenetic analysis followed by the assessment of haematologic analysis,
immunological biochemical status of infected dogs. The blood of 242 police dogs was
collected for parasitological and molecular diagnosis in addition to hematological,
biochemical, and oxidative stress analysis of the blood were performed with gene
expression analysis using two genes (gamma interferon [IFN-γ] and tumor necrosis
factor alpha [TNF-α]). The 18S rRNA region of canine Babesia species. was
successfully amplified, and sequencing data were deposited in GenBank (accession
number: MT565474.1), which resembled those of B. vogeli. The results of blood
samples revealed that out of the 242 blood samples, 62(25.62%) were positive for B.
vogeli infection. Also the study aimed to morpho-molecular identification using 18S
rRNA gene (H. canis) and evaluated the blood parameters, biochemical assays of police
dogs infested with ticks from k-9 kennels in police academy of Egypt .Ticks and blood
samples were collected from police dogs were infested with ticks for detection of H.
canis and morphological identification of ticks. H. canis was detected in neutrophils and
monocytes in Giemsa stained blood smears using light microscopy. Serum biochemical
assays were done by spectrophotometric methods (urea, creatinine, ALT, AST and
serum protein) and blood parameters were examined in the infected blood samples.
Polymerase chain reaction (PCR) amplified a " ~ "700 bp fragment of the 18S rRNA gene
of Hepatozoon spp. and the DNA products were sequenced. Resulting sequences were
compared to each other and with other available representative mammalian carnivore
Hepatozoon sequences from GenBank. The present accession number in this study were
registered in Egypt for the first time in Genebank as (MW362244.1_ MW362245.1).
Sequences of the 18S rRNA gene of H. canis were 99.98% –100.0 % identical to those
registers in GenBank. Histopathology form infected freshly died police dogs revealed
the appraisal tissues lesions and were described.
Prevalence of Hepatozoon canis was detected in (5%) of dogs by direct
micrscopy while it was (10%) by PCR and sequencing. Histopathology revealed
that 5 dogs out of 25 died dogs infested with ticks were infected by Hepatoozoon
canis, Tick species were identified morphologically as (R. sanguineus).