الفهرس 
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Abstract
Cynanchum acutum is a wild perennial herb commonly distributed in Egypt around canal bank edges of cultivation areas of the River Nile and Mediterranean regions, it also grows in tropical, subtropical and temperate area of Europe and Asia. It is commonly known as ―Olliq, modeid or libbein‖.
The broad aim of this thesis was to conduct a phytochemical and biological study of the plant Cynanchum acutum, Family: Apocynaceae. The choice of the plant was based on the promising reported biological activities of Cynanchum acutum plant extract as anti-diabetic and anti-lipidemic agent. Additional aims include biological evaluation of the pure compounds isolated from the plant to investigate the potential use of these compounds in the treatment and /or prevention of diseases.
The initial part of the work focused on extraction of the plant with different solvents, followed by purification of the different fractions and isolation of pure compounds. Seven compounds are reported and identified as rutin (1), Quercetin -3-O-neohesperidoside (2), quercetin-3-O-β-galactoside (3), isoquercitrin (4), quercetin (5), kaempferol 3-O-β-glucoside (6), and scopoletin (7). The isolated compounds were characterized by using different spectroscopic methods including EI MS, ESI MS, H1-NMR, C13-NMR and compared with the data reported in literature of the related known compounds.
The second part of the work focused on metabolic profiling of the C.acutum extract which was performed using LC-ESI-HR-MS and revealed the presence of secondary metabolites as flavonoids , flavones , alkaloids and coumarins.
The third part of the work focused on in- vivo study of some of isolated compounds. The first five compounds were selected for in vivo investigation of their anti-inflammatory and antioxidant properties in a rat model of type 2.