الفهرس | Only 14 pages are availabe for public view |
Abstract Medicine plants have been an integral part of human society since the early beginning of civilization for the treatment of diseases. In traditional literature such as that of ancient Egyptian and Persian Aloe Vera’s therapeutic advantages and healing properties have been appreciated for generations. The aim of this study was to determine the effect of Aloe Vera leaf extract on the cells of Swiss albino mice. We focused on the mitotic index and chromosomal abnormalities in mouse bone marrow cells. A complete blood count was also performed to detect any additional effects of aloe leaf extracts. In cages made of polypropylene, 32 mice were kept. Throughout the experiment, the animals were given a conventional diet, water at will, and bedding materials made of wood sawdust and shavings. For four weeks, three groups received daily oral treatments using gavage needles containing freeze-dried powdered alcoholic Aloe Vera extract in doses of 40, 80, and 120 mg/kg in distilled water, respectively. Three additional groups received daily oral treatments using the same dose plus 100 mg/kg of ARA-C (Tabine® 1000 mg/10 ml) administered subcutaneously. One group served as a positive control and received daily oral treatments using distilled water, while the other group served as a negative control. The results showed the following: 1. The mitotic index was significantly reduced by Aloe Vera leaf extract to a level lower than the negative control. 2. ARA-C reduced the mitotic index to about a third of the negative control. 3. The combination of ARA-C with different concentrations of Aloe Vera leaf extract decreased the mitotic index at the concentrations of ARA-C + 40 mg/kg and ARA-C + 80 mg/kg Aloe Vera leaf extract. However, the concentration of ARA-C + 120 mg/kg Aloe Vera leaf extract induced a significant increase in the mitotic index. 4. Aloe Vera leaf extract alone reduced chromosomal aberrations, but this reduction is not dependent on the concentration of Aloe Vera leaf extract. 5. ARA-C induced a significant increase in chromosomal aberrations. ARA-C + 80 mg/kg and 40 mg/kg Aloe Vera leaf extract reduced the chromosomal aberrations, and a great reduction was observed with ARA-C + 120 mg/kg Aloe Vera leaf extract. 6. The highest frequency of Micronucleus was observed in mice treated with ARA-C, followed by ARA-C + 120 mg/kg Aloe Vera leaf gel extracts and ARA-C + 40 mg/kg Aloe Vera leaf gel extracts. Which indicates that Aloe Vera leaf gel extracts reduced the micronucleus ratio to 1/10 of the ratio of ARA-C alone. 7. White blood cells were significantly increased by Aloe Vera extract, and it has the potential to counteract the negative effects of ARA-C treatment but does not reach the negative control level. And this effect is not dose-dependent. 8. Measuring Red blood cell count (RBC) shows a significant decrease from the negative control in Aloe Vera 40 mg/kg and Aloe Vera 80 mg/kg. An insignificant decrease in Aloe Vera 120 mg/kg. A significant increase from ARA-C treated mice in ARA-C + Aloe Vera 55 40 mg/kg and ARA-C + Aloe Vera 120 mg/kg treated mice but in ARA-C + Aloe Vera 80 mg/kg showed an insignificant decrease. 9. A significant Hemoglobin level (HGB) increase is shown in ARA-C +Aloe Vera 120 mg/kg over ARA-C, ARA-C + Aloe Vera 40 mg/kg and ARA-C + Aloe Vera 80 mg/kg treatments. And no significant difference between Control, Aloe Vera 40 mg/kg, Aloe Vera 80 mg/kg and Aloe Vera 120 mg/Kg. 10. A significant Lymphocytes count increase in Aloe Vera 80 mg/kg and Aloe Vera 120 mg/Kg than the negative Control. And The ARA-C +Aloe Vera 120 mg/kg treatment shows a significant increase over ARA-C, ARA-C + Aloe Vera 40 mg/kg and ARA-C + Aloe Vera 80 mg/kg. 11. Significant increases in uric acid levels appear in Aloe Vera 80 mg/kg, Aloe Vera 120 mg/kg, ARA-C, ARA-C + Aloe Vera 40 mg/kg, ARA-C + Aloe Vera 80 mg/kg, and ARAC + Aloe Vera 120 mg/kg more than the negative control. And significant decreases appear in ARA-C + Aloe Vera 40 mg/kg, ARA-C + Aloe Vera 80 mg/kg, and ARA-C +Aloe Vera 120 mg/kg more than the ARA-C treatment. The obtained result revealed that more research is required to properly comprehend the active principles and the precise mechanism of action of Aloe Vera leaf extract because the data that have been collected are insufficient to evaluate Aloe Vera components. |