الفهرس 
Diagnosis of Subclinical Mastitis Using Different Methods, with Isolation of Different Causative Organisms from Cow’s Milk
ABSTRACTOnly 14 pages are availabe for public view
 |  | from | 174 |  |  |
| | | from | 174 | | |
Abstract
A total of 214 bulk tank milk (BTM) samples and a total of 832 individual quarter milk samples
(IQMS) of clinically recognized and apparently normal quarters of 208 lactating cows were
collected from different dairy farms in Alexandria, Menofia and El-Dakahlia Governorates, Egypt.
Bulk tank somatic cell count and associated risk factors influencing the hygienic quality and
chemical composition of raw milk were investigated. Different mastitis indicators as screening tests
(California Mastitis Test ”CMT” and Somatic Cell Count ”SCC”); milk constituents; pH value;
enzymes activities (milk lactate dehydrogenase LDH, Acid phosphatase ACP, alkaline phosphatase ALP and glutathione peroxidase GPx) and bacteriological examination were applied on IQMS to
assess the potential relationships between these parameters and their possibility of being markers
for diagnosis of mastitis. In BTM analysis, the mean values of SCC/ml, Aerobic Plate Count APC
(cfu/ml), Coliform count (MPN/ml), S. aureus count and B. cereus count (cfu/ml) were
6.85×105
±0.33×105
, 7.09×106
±2.76×106
, 2.77×104
±0.63×104
, 1.27×103
±0.25×103
and
3.32×103
±0.13×103
, respectively. The results reveal the presence of S. aureus; CNS; St. uberis, E.
coli and B. cereus with different percentages from the examined samples. In the level of IQMS, the
mean SCC of all positive and negative CMT samples was 8.78×105
±3.49x104
and
1.63×105
±6.29x103
cells/ml respectively. The mean values of milk fat, protein, SNF, lactose and
ash content were significantly higher in IQMS from normal quarters than those from subclinical or
clinical ones; while chlorine %, pH values and enzymes activity increased with the progress of
infection. S. aureus, E. coli, coagulase negative staphylococci (CNS), St. uberis and other
streptococci could be isolated at varying percentages from the IQMS. Meanwhile, Trueperella
pyogenes, St. agalactiae and St. dysgalactiae failed to be isolated from any of the examined IQMS.
Consequently, LDH and ACP proved to be better and more reliable biomarkers for early diagnosis
of subclinical mastitis.