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Abstract
Potato virus Y (PVY), a member of Potyvirus genus, is one of the most important potato viruses and is the causal agent of several plant diseases in a wide range of host species, causing important economic losses in agriculture. These studies were conducted during the period from 2013 to 2016 at the Department of Plant Biotechnology, National Research Center, Cairo, Egypt, to identify and detect the proteins involved in response to PVY infections in N.tabacum Samsun CV. In the first experiment, Total soluble proteins were extracted from N.tabacum leaf tissue inoculated with PVY, Protein changes in both healthy and inoculated tobacco leaves were revealed using one dimensional gel electrophoresis (1D-SDS). Proteins that were highly and significantly changed are selected for identification by liquid chromatography mass spectrometry (LCMS/ MS) combined with bioinformatics. These data provide a valuable resource for discovering novel proteins involved in the pathogen response. Such proteins could be introduced into agronomical important species to develop a viral resistant crop plants. A total of 470 proteins were confidently identified, with a predominance of proteins associated with photosynthesis, energy and metabolism and response to stimulus. The second experiment was conducted to study the role of PAP in plant response against PVY infection using N.tabacum cv. Samsun. To achieve this goal, pokeweed micropropagation protocol was achieved. After 12 days of inoculation, N. tabacum plants pre-treated with 0.1 mg/mL pokeweed crude leaf extract before inoculation with PVY showed less symptoms compared to that inoculated with PVY only. Furthermore, the leaf total soluble proteins of PAP treated and untreated PVY infected tobacco plants were analyzed by 1DSDS