الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
 |  | from | 45 |  |  |
| | | from | 45 | | |
Abstract
Objective:
To study the effect of cryopreservation process on the DNA fragmentation index of sperms in patient with oligoathenoteratozoopermia.
Methods:
We prospectively assess the DNA fragmentation index of 30 patients with OATS in fresh semen samples, and we do cryopreservation on part of this samples. after that we reassess the DNA fragmentation index on the cryopreserved samples to compare the DNA fragmentation index difference between fresh and cryopreserved samples.
Results:
Mean age was 37.23and Mean BMI was 27.70. Average sperm count was 3.34 million per ejaculate . Period of cryopreservation and abstince was 5 days and 3 days. About two third of patients were smokers. Around one forth (23.3%) of patients having 1% of normal sperm motility, half of patients having 2% and the last one forth of patients (23.3%) having 3% of normal sperm motility.
Mean percentage of DNA fragmentation index in fresh samples was 22.83 (SD 8.40). Post- cryopreservation, mean percentage increased to 39.64 (SD 19.37).
Mean difference was 16.81(SD 16.59). This corresponds to a statistically significant percentage increase of about 17%.
Conclusion:
In oligoasthenoteratozoospermic patients and cancer patients enduring gonad toxic radiation and chemotherapy drugs, cryopreservation of semen is recommended for fertility preservation. Cryopreservation of sperm samples is sometimes done as a backup for the sample’s intended use on the day of IVF or ICSI procedures. Despite the fact that cryopreservation greatly increases the DNA fragmentation level of washed sperm, we continue to use the cryopreservation facility since the advantages much exceed the risks.
CONCLUSION
Cryopreservation of semen is indicated for fertility preservation in oligoasthenoteratozoospermic patient and in young cancer patients undergoing gonad toxic radiotherapy and chemotherapeutic agents. It is also beneficial prior to performing a vasectomy and in certain medical conditions which cause loss of testicular function. In some situations, cryopreservation of semen samples is done as a backup for the intended use of the sample on the day of the IVF or the ICSI procedures. Despite the fact that cryopreservation greatly increases the DNA fragmentation level of washed sperm, we continue to use the cryopreservation facility since the advantages exceed the risks.