الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
” This work study mainly aims to Control of some Multidrug Resistant Bacteria Using Zn-O Nanoparticles Synthesized by Different Methods in addition to their antioxidant, antimicrobial and cytotoxic activities, the following items were included: Obtaining some human pathogenic bacteria from MU hospital. Purification and identification (Morphological, biochemical and molecular) of the obtained pathogenic bacteria,Collection of some medicinal plants (Launaea mucronata, and Iphiona mucronata) grown naturally in Egyptian habitats. Preparation of plant extracts and phytochemical analysis of selected plant species by GC-MS. The Zn-O Nps are synthesized chemically and via green synthesis method by using extracts of Launaea mucronata, and Iphiona mucronata as reducing and stabilizing agents. characterization of Zn-O nanoparticles by TEM , Zeta potential and UV- Visible Spectrophotometer, Studying the antioxidant, antimicrobial and cytotoxic activities of plant and its metal nanoparticles . A total of 120 clinical samples were collected during the 2021 year from Mansoura University Hospital. The clinical samples included blood (40), urine (35), and stool (45) from different patients with different genders and ages ranging from 16 to 55 years. The studied isolates were differences with other in colony morphology. A large variety of both Gram-negative and Gram-positive bacteria are involved. This bacterial species is a member of the ESKAPE group recently described as the main contributor to the health human infection problem. In this study, the obtained isolates were biochemically characterized according to enzymatic activity and function properties. The quantitative analysis of samples were a rich source of phenols, alkaloids, and flavonoids. The antioxidant activity of the aqueous extract of were in vitro evaluated by DPPH assay. Based on the results of IC50, the antioxidant activity of two plant samples follows the sequence I.mucronata ˃ L.mucronat . the antimicrobial potentiality of I. mucronata and L mucronata aqueous extract in addition to the prepared zinc nanoparticles solutions (biological and chemical prepared) were tested against bacterial species. The aqueous extracts of two plants expressed no antimicrobial activity but zinc nanoparticles of the aqueous extract of plants show activity against Gram-negative bacterial strains, the cytotoxic activity were evaluated using an MTT assay. The samples were tested in vitro against three tumor cells, i.e., HepG-2, MCF-7 and PC3 cell lines. Based on the results of IC50, the cytotoxic activity of plant extracts and its nanoparticles follows the sequence Zn-O-NPs > plant extract.