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العنوان
Antagonistic effect of bacillus subtilis in biocontrol of fusarium wilt in cucumber /
الناشر
Esraa Hafez Saad Hafez ,
المؤلف
Esraa Hafez Saad Hafez
هيئة الاعداد
باحث / Esraa Hafez Saad Hafez
مشرف / Mary Sobhy Khalil
مشرف / Nahed Zakaria Haikal
مشرف / Mary Sobhy Khalil
تاريخ النشر
2018
عدد الصفحات
119 P. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علوم النبات
تاريخ الإجازة
30/9/2018
مكان الإجازة
جامعة القاهرة - كلية العلوم - Botany - Microbiology
الفهرس
Only 14 pages are availabe for public view

from 160

from 160

Abstract

In this study it is attempted to investigate the antagonistic mechanisms of Bacillus subtilis against Fusarium oxysporum, which is the cause of cucumber wilt disease. The fungal organism was isolated from the infected rhizosphere of cultivated area with cucumber plants. Pot experiments were designed, firstly by seeds treatment, spraying and irrigated with crude extract and culture of Bacillus subtilis and biofertilizers. The results revealed that the best treatment was the combination of biofertilizer1 (sting) and the Bacillus subtilis crude extract, which is significantly promoting the growth of cucumber plants and decrease the incidence of disease (83.8%). The antifungal compounds were determined from the crude extract of Bacillus subtilis. The extract was purified by ammonium sulphate and identified using Gas chromatography Mass spectroscopy (G-C Mass). This compoundwas identified as pseudojervine, rescinnamine, O,N-permethylated-N-acetyllisine and scopoletin