الفهرس | Only 14 pages are availabe for public view |
Abstract It was interesting to apply different tissue culture techniques for micropropagation of valuable and threatened medicinal plants used in traditional medicine and belonging to two different families and habitats. The study is divided into two parts: Part (I): Forest plants belonging to family podocarpaceae including [podocarpus macrophyllus (Thunb.), podocarpus gracilior (Pilg.), podocarpus elongatus (Aiton) Lʼ Hér. ex Pers]. Part (II): A wild desert plant belonging to family capparacea (capparis spinosa L.). Botanical study and genetic profile of podocarpus species were conducted. In vitro propagation of podocarpus species through tissue culture experiments was done and includes (sterilization, start, micropropagation, subculture, rooting, acclimatization, callus formation, studying the effect of stress factors (Elicitors) on shootlets growth experiments). Phytochemical screening, investigation of the volatile constituents of the leaves of Podocarpus species and identification of the major constituents by GC/MS were also carried out. Investigation of lipoidal matter (saponifiable and unsaponifiable matter) of podocarpus species was done. Also quantitative determination of phenolic content and flavonoids of methanolic extracts of Podocarpus species was done using both colorimetric assay and HPLC analysis. Fingerprint study of different fractions of both leaves and stems of Podocarpus species by HPTLC was carried out where kampferol, apigenin, luteolin and quercetin were identified in different fractions |