الفهرس 
Title: Production of ?- galactosidase Enzyme and it’s Industrial Application
AbstractOnly 14 pages are availabe for public view
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Abstract
Twenty strains of lactic acid bacteria (LAB) were isolated from Kareish cheese in Cairo markets. The results indicated that Streptococcus thermophilus and Lactobacillus bulgaricus were the most efficient Ý-gal activity producers with specific activity of 2.50 and 2.09 U/mg protein, respectively. These strains were immobilized by barium alginate 2% entrapped cells. Ý- Galactosidase from These strains was immobilized on barium alginate 2% and activity and stability were evaluated. Lactose free milk produced from Skimmed Milk by immobilized these strains using fed-batch culture. L. bulgaricus 10% showed the most efficiency of 85.26%. Lactose free milk produced by immobilized Ý- Galactosidase using continuous process with four flow rate. The flow rate 0.1ml/min was the most efficient (92.89 %). Saccharification of whey lactose by immobilized Ý-Galactosidase using continuous process with flow rate 0.25 ml/min gave efficiency of (73.05%).in conclusion immobilized bioreactor containing immobilized enzyme is recommended for continuous production of lactose free milk at flow rate 0.1 ml/min