الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
The conventional therapy for HNC includes surgical resection of operable tumors, succeeded by chemo/radiotherapy. Unfortunately, this approach has a serious multi-system toxicity profile and organ damage Treatment paradigms of HNC are constantly unfolding to novel modalities that can preclude the toxicities associated with the conventional treatment. One of these looming treatment modalities is by utilizing MSC-derived exosomes.
The aim of our study was to investigate the effect of ADSC-derived exosomes in HNSCC cell line in respect to metastasis and angiogenesis by evaluating the expression of CRKI, VEGF and miR-200 genes.
In the present study, two different concentrations (10 and 20 ng/ml) of exosomes derived from adipose-derived mesenchymal stem cells (ADSCs), after isolation and culturing, were used in treatment of cultured HEp-2 laryngeal cancer cells at 24 and 48 hour durations.
The isolated exosomes were characterized using western blot technique and visualized via transmission electron microscopy (TEM) before internalization into the cultured cancer cells.
Six cell culture subgroups were classified in this study depending on different durations and concentrations.
At both durations (24 and 48 hours), treatment with MSC-derived exosomes significantly decreased the mean viability percentage of HEp-2 cells, indicating the effectiveness of the treatment in inhibition of cancer cells proliferation, although the used concentrations were statistically insignificant to each other.
Regarding the qRT-PCR results, at both durations (24 and 48 hours), treatment with MSC-derived exosomes significantly decreased CRKI gene expression, indicating the effectiveness of the treatment in reducing invasiveness HEp-2 cells, although the used concentrations (10 and 20 ng/ml) were statistically insignificant to each other.
Regarding the qRT-PCR results of VEGF genes expression, at both durations (24 and 48 hours), treatment with MSC-derived exosomes significantly decreased VEGF gene expression, especially the higher concentration in the case of shorter duration, indicating the effectiveness of the treatment in reducing angiogenesis of HEp-2 cells, although the used concentrations (10 and 20 ng/ml) were statistically insignificant to each other.
Regarding the qRT-PCR results of miR-200 genes expression, at both durations (24 and 48 hours), treatment with MSC-derived exosomes significantly increased miR-200 gene expression, indicating the effectiveness of the treatment in reducing invasion and angiogenesis of HEp-2 cells, although the used concentrations (10 and 20 ng/ml) were statistically insignificant to each other.
Based on the results of the present study, it could be concluded that treatment of HNSCC cell line with ADSC-derived exosomes was most efficient at concentration of 20 ng/ml for 48 hours.