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Abstract This experiment was conducted at the experimental nursery of the Ornamental Horticulture Department, Faculty of Agriculture, Cairo University, Giza, during the two successive seasons of 2014/2015 and 2015/2016 seasons. The aim of present research was to investigate the effect of some bio-stimulants and micronutrients mixture and their interactions on vegetative growth, yield, essential oil production and chemical compositions of Majorana hortensis plants. The plants were treated with actosol as bio-stimulant applied monthly as soil drench at the concentrations of 0.25 and 0.50 ml/l, in addition to control and the same plants were foliar sprayed with micronutrients mixture (Fe+ Mn + Zn) applied monthly at the concentrations of 30 and 60 ppm in addition to control that sprayed with tap water. The results showed that; Actosol treatments significantly increased plant height, fresh and dry weights of herb in the first and third cuts, root length, number of roots/plant, oil percentage and oil yield (at the third cut), content of chlorophyll a and carotenoids, total carbohydrates % (at the second season of third cut) as well as Fe, Mn and Zn content in dry herb as compared to the control. N and K% in dry herb were increased significantly with Actosol at high concentration, while P% was increased significantly with Actosol at low concentration as compared to the control. On the other hand, fresh and dry weights of herb in the second cut, fresh and dry weights of roots, oil yield (at the first season of second cut) chlorophyll b content (in first seasons) and total carbohydrates % (at the second cut) were reduced significantly with application of Actosol treatments as compared to the control |