الفهرس 
Title : Isolation and screening of fungi producing L-asparaginase in Egypt
ABSTRACTOnly 14 pages are availabe for public view
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Abstract
One hundred and forty three isolates related to 16 fungal species were recovered from garden soil and indoor air of the medical chambers of tumor institute, Kasr El Ainy, Egypt. Quantitative L-asparaginase assay for the isolated species was determined by Nesslerization. The fungal isolates exhibited different L-asparaginase activities. Aspergillus sydowii and Fusarium oxysporum showed the highest L-asparaginase activity where the activity reached 3.98 and 3.91 U/mL in the assay mixture after 48h incubation period, respectively. The identification of A. sydowii and F. oxysporum was confirmed molecularly using Internal Transcribed Spacer Regions sequences. The optimization of the cultural conditions for the production of L-asparaginase was performed by using Taguchi design. Eight component factors of a modified medium were evaluated in influencing L-asparaginase yield. The highest L-asparagenolytic activity was achieved in culture media containing 1% L-asparagine, 0.2% glucose, 0.1% KH2 PO4, 0.025% KCl, 0.052% MgSO4. 7H2O, pH 5.0 and incubation at 40 oC in a rotatory incubator (100 rpm) for 5days, where enzyme activities of 146 and 143 U/mL were measured for A. sydowii and F. oxysporum, respectively