الفهرس 
Immunohistochemical expression of octamer-binding protein-4, in oral epithelial dysplasia induced in experimental rats :Animal study
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Abstract
OSCC is one of the most frequent cancers in the world, which is thought to arise from progressive dysplastic changes of oral squamous epithelium. This study was performed to assess the immunohistochemical expression of OCT4 in OED in order to explore its role in oral carcinogenesis, and its possible use as a novel marker for detection of early molecular changes prior to malignant transformation. A total of 84 adult male albino rats with an age range of 3 to 4 months and a weight range of 100 to 200 gm were selected. The rats were randomly distributed into two groups: control group (comprised 28 rats which did not receive any induction of carcinogenesis) and experimental group (comprised 56 rats which had their buccal mucosae painted with DMBA and formaldehyde every alternate day). Half the number of rats of both groups was euthanized at 6th and 9th week intervals, respectively. The dissected buccal mucosae of rats were processed routinely to obtain 5o thick sections. These sections were histologically stained by H&E stain and immunohistochemically by anti-OCT4 mouse monoclonal antibody. In the current study, the stained sections were examined by ordinary light microscope to detect the dysplastic changes and identify the low and high risk groups. Moreover, the image analyzer computer system was used for accurate measuring of the area percentage of OCT4 immunoreactivity in the examined sections using the software Leica Qwin 500. The mean values of the obtained data were statistically evaluated using ANOVA test to compare the area percentage of positive OCT4 immunoreactivity among the examined groups (control, low, high risk OED). Then, Tukey{u2019}s post-hoc tests were performed for pair-wise comparisons between each two groups