الفهرس 
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Abstract
Introduction: Complete eradication of microorganisms from the space of root canal is the main goal for the successful root canal treatment and the health of periradicular tissue . Objective: To assess the antibacterial efficacy of sodium hypochlorite (NaOCl) and 980 nm diode laser and PDT with chitosan on infected root canals with Enterococcus ”E” Faecalis biofilms. Material and Methods: One hundred and five upper anterior incisors human teeth with single root and root canal were instrumented by protaper system up to F3. Biofilms of E. faecalis were generated based on a previously established protocol. Five teeth were used to check the biofilm formation, then the remaining teeth were randomly divided into two lines according to the disinfection methods; line A: high power diode laser ,line B: photodynamic therapy. The antibacterial efficacy was evaluated by colony forming units, and relative intensity of live/dead bacteria, using Confocal Laser Scanning Microscopy . Data were analyzed by proper statistical analysis with P= 0.05. Results: Regarding the disinfection method, all groups in two lines had a significantly high percentage of bacteria reduction (P<0.05). However, the highest bacterial reduction was achieved with the combination of 2.5٪ NaOCl and diode Laser followed by the combination of 2.5٪ NaOCL and PDT (P<=0.001). Conclusion: Under the conditions of the present study, results reinforced that 980 nm diode laser irradiation is a useful adjunct in disinfection of infected root canals with E. Faecalis biofilms.